Promotion of seed germination traits and seedling growth of pot marigold (Calendula officinalis L.) by Pseudomonas spp.

Document Type : Research Paper

Authors

1 Bsc student, Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

3 Associate professor, Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

10.22124/jms.2023.7672

Abstract

Plant growth promoting rhizobacteria are a group of bacteria that can actively colonize plant roots and can stimulate plant growth. This study was executed to evaluate of four strains of Pseudomonas fluorescens Migula (PF1-4) and six strains of Pseudomonas putida (Trevisan) Migula (PP 1-6) on seed germination characteristics including germination percentage, germination rate, root and shoot length and dry weight, seed and seedling vigor index and α-amylase activity of pot marigold (Calendula officinalis L.). In vitro production of siderophore, hydrogen cyanide, indole-acetic acid (IAA) and volatile and non-volatile metabolites of bacteria were also determined. The bacterial suspension (109 CFU/ml) was used to inoculate the seeds under aseptic conditions. Results revealed that the metabolites concentrations were significantly different between bacterial strains. Different strains of rhizobacteria had also variable effects on germination parameters and the effect of plant-rhizobacteria interactions on germination characteristics could be positive or neutral. Maximum effects of bacterial strains were recorded for PP-1, PP-2, PP-5, PF-1 and PF-2 strains. The effects of bacterial strains on studied characteristics were significantly correlated with IAA production by bacteria. As a conclusion, the role of biopriming with growth promoting rhizobacteria on germination characteristics and seedling growth were dependent on bacterial species and strains. These results also suggest that the amont of bacterial IAA produced plays a major role in seed germination and may stimulate germination features.

Keywords

References

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Iranian Journal of Seed Sciences and Research
Volume 10, Issue 3
October 2023
Pages 21-32

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