The effect of priming with Ellagic acid on the seed reserves mobilization and the growth of soybean seedlings under accelerated aging

Document Type : Research Paper

Authors

1 Ph D. student of Agronomy, Shahrood University of Technology, Shahrood, Iran

2 Department of Agronomy, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran

10.22124/jms.2022.6162

Abstract

In order to investigate the effect of ellagic acid on the growth of seedling and the qualities of soybean in accelerated aging conditions, a pilot was designed in 2019 in the research laboratory of the Shahrood University of Technology. The treatments included aging seed in two levels (unaged seeds and aged seeds) and priming at seven levels (control, distilled water, 25, 75, 50, 100, 125 mg/L with ellagic acid). A pilot study as a factorial experiment with a completely random-base design (CRD) in three replications was carried out under a germinator environment in accordance with standards. To prepare aged seeds in the laboratory, the seeds were incubated at 41°C and 95% relative humidity for 72 hours. Seed priming was soaking with ellagic acid for 6 hours in accordance with the principles of seed aeration. The aging reduced Vigor Index, Allometric Growth Ratio and Seed Reserves Use Rate. The increasing of the malondialdehyde had a negative effect on germination percentage through the influence of Vigor Index and α-amylase activity enzyme. The application of ellagic acid increased the germination percentage in unaged seeds and aged seeds through increasing the activity of α-amylase activity enzyme. The priming of seeds with ellagic acid reduced the malondialdehyde and electrical conductivity. Based on the results of research, the application of 50 mg/L of ellagic acid can be introduced as the best concentration of this material. It can also be suggested that the use of ellagic acid as a seed priming improves the effects of seed aging on soybeans.

Keywords

References

Abdul Baki, A. and Anderson, J.D. 1973. Vigour determination in soybean by multiple criteria. Crop Science, 13: 630- 633. (Journal)
Abu El Soud, W., Hegab, M.M., Abdelgavad, H., Zinta, G. and Asard, H. 2013. Ability of ellagic acid to alleviate osmotic stress on chickpea seedlings. Journal of Plant physiology and biochemistry, 71(1): 173-183. (Journal)
Adetunji, A.E., Adetonji, G.L., Varghese, B. and Pammenter, N. 2021. Oxidative Stress, Ageing and Methods of Seed Invigoration: An Overview and Perspectives. Agronomy, 11 (2369): 1-27. (Journal)
Ahmadloo, F., Tabari, M. and Behtari, B. 2012. Effect of water stress and accelerated ageing on some physiological characteristics of Pinus brutia Ten. Seeds. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 19 (2): 345-358. (In Persian) (Journal)
Akbari, M., Baradaran firouzabadi, M., Amerian. M.R. and Farrokhi, N. 2020. Seed Pretreatment with Cinnamic Acid Positively Affects Germination, Metabolite Leakage, Malondialdehyde Content and Heterotrophic Growth of Aging Cowpea (Vigna unguiculata) Seeds. Iranian Journal of Seed Research, 6(2): 163-176.
Ascacio-Valdes, J.A., Aguilera-Carbu, A., Martinez-Hernandez, J.J., Rodriguez-Herrera, R. and Aguilar, C.N. 2010. Euphorbia antisyphilitica residues as a new source of ellagic acid. Chemical paper, 64: 528-32. (Journal)
Balouchi, H.R., Bagheri, F., Kayednezami, R., Movahedi, D.M. and Yadavi, A.R. 2014. Effect of seed aging on germination and seedling growth indices in three cultivars of Brassica napus L. Journal of plant research, 26(4): 397-411. (Journal)
Cai, J.B., Xu, D., Liu, Y. and Zhao, N.N. 2008. Sensitivity analysis on water deficit indicator of winter wheat based on path analysis theory. Journal of Hydraulic Engineering, 39(1): 83-90. (Journal)
Damaris, R.N., Lin, Z., Yang, P. and He, D. 2019. The rice alpha-amylase, conserved regulator of seed maturation and germination. International Journal of Molecular Sciences, 20(2): 450-459. (Journal)
Daneshvar Rad, R., Sadrghipoor, O. and Pazeki, A. 2015. ffect of soybean seed hydropriming on germination percentage and average in vitro and yield and yield components in field under drought stress, Iranian Seed Science and Technology, 3(1): 53-66. (In Persian) (Journal)
Debnath, B., Singh, W.S., Das, M., Goswami, S. and Manna, K. 2021. Biodynamic activities of ellagic acid: A Dietary Polyphenol. Journal of Nature and Science of Medicine, 3(20): 83-90. (Journal)
Du, Z. and BramLey, W.J. 1992. Modified thiobarbituric acid assay for measuring lipid oxidation in sugar-rich plant tissue extracts. Journal of Agricultural and Food Chemistry, 40(9): 1566- 1570. (Journal)
Ebone, L.A., Caverzan, A., Tagliari, A., Chiomento, J., Silveira, D. and Chavarria, G. 2020. Soybean seed vigor: uniformity and growth as key factors to improve yield. Agronomy, 10(545): 1-15. (Journal).
Farhadi, N., Estaji, A. and Alizadeh-salteh, S. 2016. The Effect of Pretreatment of Salicylic Acid on Seed Germination of Milk thistle (Silybum marianum cv. Budakalaszi) Under Salinity and Drought Stress. Iranian Journal of Seed Research, 3(1): 75-83. (Journal)
Ghaderi-Far, F., Soltani, A. and Sadeghipour, H.R. 2014. Biochemical changes during ageing in medicinal pumpkin: lipid peroxidation and membrane damage. Iranian Journal of Plant Biology. 20(6): 96-112. )In Persian( (Journal)
Ghanbari, M., MakhtassiBidgoli, D.,TalebiSiahsaran, P. and Pirani, H. 2019. Effect of deterioration on germination and enzymes activity in dry bean (Phaseolus vulgaris L. cv. Sadri) under salinity stress condition. Environmental Stresses in Crop Sciences, 12(2): 585-594. (In Persian) (Journal)
Gholami, T.B.H., Salehi, B.M. and Farhadi, R. 2012. Priming effect on germination and seedling growth of aged grain of rice (Oryza sativa L.). Seed Research (Journal of Seed Science and Technology), 2(1): 1-13. (In Persian) (Journal)
Hampton, J.G. and TeKrony, D.M. 1995. Handbook of vigor test methods. The International Seed Testing Associastion, Zurich. (Handbook)
 
ISTA (International Seed Testing Association). 2009. International rules for seed testing. Seed Science and Technology, 49: 86-41. (Journal)
 
 
Kapilan, R. 2015. Accelerated aging declines the germination characteristics of the maize seeds. Journal of Biosciences, 3(8): 708-711. (Journal)
Kavandi, A., Jafari, A.A. and Jafarzadeh, M. 2018. Effects of osmopriming on the enhancement of seed germination and seedling growth of deteriorate seeds of sainfoin (Onobrichis viciifolia) in basic and active collections of gene bank, Iranian Journal of Range and Desert Research, 25(3): 671-685. (In Persian) (Journal)
Khan, A., Nazar, S., Lang, I., Nawaz, H. and Hussain, M.A. 2017. Effect of ellagic acid on growth and physiology of canola (Brassica napus L.) under saline conditions. Journal plant interaction, 12(1): 520-525. (Journal)
Liu, Y., Liu, Y., Liu, H. and Shang, Y. 2018. Evaluating effects of ellagic acid on the quality of kumquat fruits during storage. Scientia Horticulturae, 227: 244–254. (Journal)
Lopez, S.G. and Luderer, U. 2004. Effects of cyclophosphamide and buthionine sulfoximine on ovarian glutathione and apoptosis. Free Radical Biology and Medicine. 36 (11): 1366-1377. (Journal)
Maesaroh, S., Wahyu, Y. and Widajati, E. 2021. Seed Storability and Genetic Parameters Estimation on Accelerated Aging Seed of Argomulyo Soybean (Glycine max (L.) Merr.) Mutant Lines. Journal of Agricultural Sciences, 31(3): 763- 775. (Journal)
Mcdonald, M.B. 2000. SEED PRIMING (eds. M. Black and J. D. Bewley). Sheffield Academic Press. PP.287- 325. (Book)
Moori, S. and Eisvand, H.R. 2019. The effect of priming with salicylic acid and ascorbic acid on germination indices and biochemical traits in wheat seed deterioration. Iranian Journal of Seed Science and Research. 6(3): 381-398. (In Persian) (Journal)
Morais, G. and Takaki, M. 1998. Determination of amylase activity in cotyledons of Faseolusvulgaros L. cv carioca. Brazilian Archives of Biology and Technology, 41(1): 17-25. (Journal)
Nahofte Esterabad, A., RahemiKarizaki, A. and NakhzariMoghadam, A. 2016. Effect of seed deterioration on germination parameters and growth seedling of two maize varieties. Iranian Journal of Seed Science and Research, 3(2): 1-11. (In Persian) (Journal)
Nazari, R., Parsa, S., TavakolAfshari, R. and Mahmoodi, S. 2020. Salicylic acid priming before and after accelerated aging process increases seedling vigor in aged soybean seed. Journal of Crop Improvement, 34(2):1-20. (In Persian) (Journal)
Pari, L. and Sivasankari, R. 2008. Effect of ellagic acid on cyclosporine A-induced oxidative damage in the liver of rats. Fundamental and Clinical Pharmacology, 22: 395–401. (Journal
Pasandideh, H., SeyedSharifi, R., Hamidi, A., Mobasser, S. and Sedghi, M. 2014. Relationship of seed germination and vigour indices of commercial soybean cultivars with seedling emergence in field. Iranian Journal of Seed and Research, 1(1): 29-50. (In Persian) (Journal)
Perry, D.A. 1991. METHODOLOGY AND APPLICATION OF VIGOUR TESTS INTERNATIONAL SEED TESTING ASSOCIATION, ZURICH, SWITZERLAND. PP. 275. (Book)
Rahemi-Karizaki, A., Nakhzari-Moghaddam, A. and Pourabdullah, M. 2012. The effect of seed vigor on germination and heterotrophic seedling growth response of wheat to salinity. Journal of Seed Science and Technology, 2(2): 60-67. (In Persian) (Journal)
Rasheed, R., Ashraf, M.A., Hussain, I., Haider, M.Z., Kanwal, U. and Iqbal, M. 2014. Exogenous proline and glycine betaine mitigate cadmium stress in two genetically different spring wheat (Triticum aestivum L.) cultivars. Brazilian Journal of Botany, 37: 399-406. (Journal)
Saadat, T., Sedghi, M., Gholipouri, A., SeyedSharifi, R. and Sheykhbaglou, R. 2020. The effect of priming deterioration on the activity of antioxidant enzymes and the mobility of seed reserves in French bean (Phaseolus vulgaris L.) cv. Sadri. Iranian Journal of Seed Science and Technology, 8(2): 19-32. (In Persian) (Journal)
Santos, R.F., Placido, H.F., Bosche, L.L., Neto, H.Z., Ferando, H. and Alessandro, B. 2021. Accelerated aging methodologies for evaluating physiological potential of treated soybean seeds. Journal of seed science, 43(4): 1-10. (Journal)
 
 
Shaaban, M. 2016. Effect of aging on enzymatic and non-enzymatic antioxidant changes and biochemical characteristics in barley (Hordeum vulgare L.) seeds cv. Valfajr. Iranian Journal of Seed Science and Research, 3(3): 89-103. (In Persian) (Journal)
Singh, B. and Chaturvedi, V.K. 2014. Impact of cinnamic acid on physiological and anatomical changes in maize plants (Zea mays L.) grown under salinity stress. Journal of Stress Physiologhy and Biochemistry, 10(2): 122-134. (Journal)
Soltani, M., Moradi, A., Tavakol Afshari, R. and Balochi, H.R. 2017. The effect of different storage conditions on germination and biochemical variability of Ricinus communis L. Iranian Journal of Field Crop Science, 48(1): 91-105. (In Persian) (Journal)
Thakur, K. and Pitre, K.S. 2008. Polarographic (DCP and DPP) determination of ellagic acid in strawberries and pharmaceutical formulations. Journal of the Chinese Chemical Society, 55: 143-46. (Journal)
Thongsri, K., Teingtham., K., Duangpatra, J. and Romkaew, J. 2021. Effects of brassinosteroids and gibberellin on water uptake and performance of soya bean seeds under different temperatures. Seed Science and Technology, 49(2): 141-157. (Journal)
Wang, R., Wu, F., Xie, X. and Yang, C. 2021. Quantitative trait locus mapping of seed vigor in soybean under −20 ◦C storage and accelerated aging conditions via RAD sequencing. Molecular Biology, 43: 1977-1996. (Journal)
Weerasekara, I., Sinniah, U.R., Namasivayam, P., Nazli, M.H., Abdurahman, S.A. and Ghazali, M.N. 2021. Priming with humic acid to reverse ageing damage in soybean (Glycine max (L.) Merrill.] seeds. Agriculture, 11(966): 1-18. (Journal)
Yeganeh, M.R., Jafari, A.A. and Sani, B. 2019. The effects of priming on seed vigority and seedling growth of deteriorated seeds in three Astragalus species, Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 27(1): 59-70. (In Persian) (Journal)
Zhang, X.S., Yi-lan, Y.A.N. and Zheng-hua, H.U. 2017. Using path analysis to identify impacting factors of evapotranspiration at different time scales in farmland, Chinese Journal of Agrometeorology, 38(4): 201-210. (Journal)
.
Iranian Journal of Seed Sciences and Research
Volume 9, Issue 3
December 2022
Pages 41-65

Files

Share

How to cite

Statistics