Effect of seed priming hormone on germination characteristics and seedling growth of Zea mays L.

Document Type : Research Paper

Authors

1 M.Sc student In science and seed technology, Ashtian Branch, Islamic Azad University

2 Associate Professor, Department of Agronomy and Plant Breeding, Islamic Azad University, Shoushtar branch, Shoushtar, Iran

10.22124/jms.2022.6144

Abstract

The aim of this experiment was to determine the effect of different concentrations of gibberellin and auxin hormones on germination and germination characteristics of maize in laboratory and greenhouse conditions in the Faculty of Agriculture, Shoushtar Branch of Azad University in the 2018-2019. Both environments were a completely randomized design with four replications. Experimental treatments included seven treatments, different concentrations (20, 40 and 60 ppm) of gibberellin and auxin hormones and distilled water as a control and soaking time was 24 hours.In the laboratory section, a significant difference was observed among the treatments in relation to the measured traits. The highest and lowest germination indices with 39.98 and 19.22, respectively, were observed in gibberellic acid and auxin 20 and 60 ppm hormone treatments. The highest final germination percentage was recorded at 20 ppm gibberellin with a 24% improvement over the control.The highest radicle and plumule lengths belonged to 40 ppm gibberellin with 92.73 and 13.97 mm, respectively. In greenhouse section, the effect of hormonal treatments on all studied traits was significant. The highest wet and dry weight with 38 and 18% increase compared to the control was observed in 20 ppm gibberellin treatment. The highest amounts of soluble and reducing sugars were obtained with 90 and 54 mg.g-1 seeds in the hormonal treatment of 20 ppm gibberellin, respectively, and the lowest values ​​were obtained with 33 and 22.4 mg.g-1 seeds in the control treatment. The highest α-amylase activity was recorded at 30.1 nmol per seed in 20 ppm gibberellin treatment. This study showed that hormone 20 ppm gibberellin were effective and efficient treatment in increasing the vigor of Fajr hybrid corn seeds.

Keywords

References

Afzal, I., Basra, S.M.A., Ahmad, N., Cheema, M.A., Warriach, E.A. and Khaliq, A. 2002. Effect of priming and growth regulator treatment on emergence. International Journal of Agriculture and Biology, 4: 306–306. (Journal)
Afzal, I., Basra, S.M.A., Shahid, M. and Saleem, M. 2008. Physiological enhancements of spring maize (Zea mays L.) under cool conditions. Seed Science and Technology, 36: 497-503. (Journal)
Agrawal, R.L. 2004. Seed technology. Oxford and IBH Publishing Co. LTD. New Delhi. (Book)
Akbari, G., Sanavy, S.A. and Yousefzadeh, S. 2007. Effect of Auxin and Salt stress (NaCl) on Seed Germination of Wheat Cultivars (Triticum aestivum L.). Pakistan Journal of Biology Science, 10: 2557-2561. (Journal)
Alonso-Ramírez, A., Rodríguez, D., Reyes, D., Jiménez, J.A., Nicolás, G., López-Climent, M., Gómez-Cadenas, A. and Nicolás, C. 2009. Evidence for a role of gibberellins in salicylic acid-modulated early plant responses to abiotic stress in Arabidopsis seeds, Plant Physiology, 150(3): 1335-1344. (Journal)
Ansari, O., Azadi, M.S., Sharif-Zadeh, F. and Younesi, E. 2013. Effect of hormone priming on germination characteristics and enzyme activity of mountain rye (Secale montanum L.) seeds under drought stress conditions. Journal of Stress Physiology and Biochemistry, 9(3): 61-71. (Journal)
Bakht, J., Shafi, M., Jamal, Y. and Sher, H. 2011. Response of maize (Zea mays L.) to seed priming with NaCl and salinity stress. Spanish Journal of Agricultural Research, 9: 252- 261. (Journal)
Basra, S.M.A., Farooq, M. and Tabassum, R. 2005. Physiological and biochemical aspects of seed vigor enhancement treatments in fine rice (Oryza sativa L.). Seed Science and Technology, 33: 623-628. (Journal)
Basra, S.M.A., Farooq, M., Wahid, A. and Khan, M.B. 2006. Rice Seed Invigoration by Hormonal and Vitamin Priming. Seed Science and Technology, 34: 775-780. (Journal)
Blackshaw, R.E. 1991. Soil temperature and moisture effects on downy brome vs. winter canola, wheat and rye emergence. Crop Science, 31: 1034-1040. (Journal)
Bradford, K.J. 1986. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Horticultural Science, 21: 1105-1112. (Journal)
Bray, C.M., Davison, P.A., Ashraf, M. and Taylor, R.M. 1989. Biochemical changes during osmopriming of leek seeds. Annals of Botany, 36: 185-193. (Journal)
Cai, T., Meng, X., Liu, X., Liu, T., Wang, H., Jia, Z., Yang, D. and Ren, X. 2018. Exogenous hormonal application regulates the occurrence of wheat tillers by changing endogenous hormones. Frontiers in Plant Science, 9: Article number 1886. (Journal)
Chakrabarti, N. and Mukherji, S. 2003. Effect of Phytohormone pretreatment on nitrogen metabolism in Vigna radiate under salt stress. Plant Biology, 46: 63- 66. (Journal)
Cohn, M.A. and Obendorf, R.L. 1978. Occurrence of a stelar lesion during imbibitional chilling of Zea mays L. American Journal of Botany, 65: 50-56. (Journal)
Coolbear, P., Grierson, D. and Heydecker, W. 1980. Osmotic pre-sowing treatments and nucleic acid accumulation in tomato seeds (Lycopersicon lycoperciscum L.). Seed Science and Technology, 8: 289-303. (Journal)
Dell’ Aquila, A. and Spada, P. 1992. Regulation of protein synthesis in germinating wheat embryos under polyethylene glycol and salt stress. Seed Science and Research, 2: 75-80. (Journal)
Ellis, R.A. and Roberts, E.H. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9: 373-409. (Journal)
 
Emem, O., Akeem, A.A., Fawibe, O., Tolulope, A., Oluwasey, A. and David, A. 2017. Effects of phytohormone on seed germination, seedling vigour and the phytochemical contents of three cucurbits. Asian Journal of Crop Science, 9:63–70. (Journal)
Farooq, M., Aziz, T., Basra, S.M.A., Cheema, M.A. and Rehman, H. 2008. Chilling Tolerance in Hybrid Maize Induced by Seed Priming with Salicylic Acid. Journal Agronomy of Crop Science, 194: 161-168. (Journal)
Farooq, M.S., Basra, M.A., Rehman, H., Ahmad, N. and Saleem, B.A. 2007. Osmo priming with salicyclic acid improves the germination and early seedling growth of melons (Cucumis melo L.). Pakistan Journal of Agricultural Science, 44: 529–533. (Journal)
Ghobadi, M., Abnavi, M.S., Honarmand, S.J., Ghobadi, M.E. and Mohammadi, G.R. 2012. Effect of hormonal priming (GA3) and osmopriming on behavior of seed germination in wheat (Triticum aestivum L.). Journal of Agricultural Science, 4(9): 244-250. (Journal)
Haroun, S.A., Badawy, A.H. and Shukry, W.M. 1991. Auxin induced modification of Zea mays and Lupinus termis seedlings exposed to water stress imposed by polyethylene glycol (PEG 6000). Science Journal, 18: 335-403. (Journal)
Hedge, J.E. and Hofreiter, B.T. 1962. Carbohydrates Chemistry. Ed. 17. Academic Press, New York. (Book)
Hoque, M. and Haque, S. 2002. Effects of GA3 and its mode of application on morphology and yield parameters of mung bean (Vigna radiate L.). Pakistan Journal of Biology Science, 5: 281-283. (Journal)
Kaur, S., Gupta, A.K. and Kaur, N. 2005. Seed priming increases crop yield possibly by modulating enzymes of sucrose metabolism in chickpea. Journal of Agronomy and Crop Science, 91: 81-87. (Journal)
Korasick, D.A., Enders, T.A. and Strader, L.C. 2013. Auxin biosynthesis and storage form. Journal of Experimental Botany, 64: 2541–2555. (Journal)
Lee, S.S. and Kim, J.H. 2000. Total sugars, α-amylase activity, and germination after priming of normal and aged rice seeds. Korean Journal of Crop Science, 45: 108-111. (Journal)
Livingston, N.J. and De Jong, E. 1990. Matric and osmotic potential effects on seedling emergence at different temperatures. Agronomy Journal, 82: 995-998. (Journal)
Marcos-Filho, J. 2015. Fisiologia de sementes de plantas cultivadas. Piracicaba: FEALQ. (Book)
Miller, G.I. 1959. Use of dinitrosalicylic acid reagent for the determination of reducing sugars. Analytical Chemistry, 31: 426-428. (Journal)
Murray, G.A. 1990. Priming sweet corn seed to improve emergence under cool conditions. Horticulture Science, 25: 231-239. (Journal)
Rivera, A.A.C., Pinho, R.G.V., Guimaraes, R.M., Veiga, A.D., Pereira, G.L. and Pinho, I.V. 2011. Efeito do ácido giberélico na qualidade fisiológica de sementes redondas de milho doce, sob diferentes condições de armazenamento. Revista Brasileira de Milho e Sorgo, 10(3): 247-256. (Journal)
Rood, S.B., Buzzell, R.I., Major, D.J. and Pharis, R.P. 1990. Gibberellins and heterosis in maize: quantitative relationship. Crop Science, 30: 281–286. (Journal)
Scalon, S.P.Q., Mussury, R.M., Scalon Filho, H., Francelino, C.S.F. and Florencio, D.K.A. 2006. Armazenamento e tratamentos prégerminativos em sementes de jacarandá (Jacaranda cuspidifolia Mart.). Revista Árvore, 30(2): 179-185. (Journal)
Shuai, H., Meng, Y., Luo, X., Chen, F., Zhou, W., Dai, Y., Qi, Y., Du, J., Yang, H., Liu, J., Yang, W. and Shu, K. 2017. Exogenous auxin represses soybean seed germination through decreasing the gibberellin/abscisic acid (GA/ABA) ratio. Scientific Reports 7: Article number 12620. (Journal)
Sung, F.J. and Chang, Y.H. 1993. Biochemical activities associated with priming of sweet corn seeds to improve vigor. Seed Science and Technology, 21: 97-105. (Journal)
Szalai, G., Horgosi, S., Soos, V., Majlath, I., Balazs, E. and Janda, T. 2011. Salicylic acid treatment of pea seeds induces its de novo synthesis. Journal of Plant Physiology, 168: 213–219.
Taiz, L. and Zeiger, E. 2013. Fisiologia vegetal. 5 ed. Porto Alegre: Artmed. (Book)
Varavinit, S., Chaokasem, N. and Shobsngob, S. 2002. Immobilization of a thermostable alpha-amylase. Science Asia, 28: 247-251. (Journal)
 
Zheng, G.H., Wilen, R.W., Slinkard, A.E. and Gusta, L.V. 1994. The enhancement of canola seed germination and seedling emergence at low temperature by priming. Crop Science, 34: 1589-1593. (Journal)
Zhou, Y., Tong, Y., Jiao, Z., Yi-tao, N.W., Qi, W., Fei, S., Wen-Juan, X.U. and Rui-dong, H. 2017. Effects of exogenous IAA application on endogenous hormone contents and tillering in sorghum. Chinese Journal of Ecology, 36:2191–2197. (Journal)
Iranian Journal of Seed Sciences and Research
Volume 9, Issue 1
April 2022
Pages 39-49

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