Evaluation of non-enzymatic antioxidant system, quantity of protein and oil of two canola cultivars (Brassica napus L.) seeds during accelerated aging conditions

Document Type : Research Paper


1 Master in Seed Science and Technology, Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 Professor, Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

3 Assistant Professor of Crop Physiology, Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

4 Assistant Professor, Department of Crop Biotechnology and Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran



In order to investigate the effect of accelerated ageing (AA) on physiological and biochemical traits of cultivar canola seeds, this study was laid out as a factorial experiments based on a completely randomized design with two factors cultivars (Modena and Okapi), aging level (0, 24 and 72 hours at temperature of 40˚c and relative humidity of saturated) and four replications in the Ferdowsi University of Mashhad at 2019-20. The results showed that the aged seeds of Okapi and Modena cultivars significant decline in germination characteristics. According to the biochemical results, in the treatments of 72 hours of AA compared to the control, oil, total protein and scavenging free radicals of canola seeds, has decreased by 9.89, 4.14 and 28.61 percent, respectively. Likewise, electrical conductivity increased by 84.64 percent. Interestingly, aging affected the content of non-enzymatic antioxidants. Here for example, it reduced flavonoids by 73.79%, proline by 47.18%, and phenol by 17.13% compared to non-aged seeds in Okapi cultivar. On the one hand, Okapi cultivar had great quality characteristics such as oil percentage, activity of antioxidant systems, but on the other hand, it was very sensitive to storage, because, aging caused extremely decline in Okapi cultivar seed quality. So, it is obvious that conditions of seed storage of this cultivar require more attention. In general, controlling storage conditions to minimize canola seed aging and deterioration is critical.


Abe, N., Murata, T. and Hirota, A. 1998. Novel 1,1- diphenyl-2- picrylhydrazyl- radical scavengers, bisorbicillin and demethyltrichodimerol, from a fungus. Bioscience, Biotechnology and Biochemistry, 62: 61-662. (Journal)
Abreu, L.A.S., Carvalho, M.L.M., Pinto, C.A.G., Kataoka, V.Y. and Silva, T.T.D.A. 2013. Deterioration of sunflower seeds during storage. Journal of Seed Science, 35(2): 240-247. (Journal)
Agati, G., Azzarello, E., Pollastri, S. and Tattini, M. 2012. Flavonoids as antioxidants in plants: Location and functional significance. Plant Science, 196: 67–76. (Journal)
Ahmadi, K., Ebadzadeh, H.R., Hatami, F., Abdeshah, H. and Kazemian, A. 2021. Agricultural statistics of the crop year 2019 Iran. Volume One: Crops. Ministry of Agriculture, Deputy of Planning and Economy, Information and Communication Technology Center. (In Persian) (Book)
Akhavan Hezaveh, T., Pourakbar, L., Rahmani, F. and Alipour, H. 2020. Effects of ZnO NPs on phenolic compounds of rapeseed seeds under salinity stress. Journal of Plant Process and Function, 8(34): 10-18. (Journal)
Alizadeh Yeloojeh, K., Saeidi, G. and Sabzalian, M.R. 2020. Drought stress improves the composition of secondary metabolites in safflower flower at the expense of reduction in seed yield and oil content. Industrial Crops and Products, 154, 112496. (Journal)
Ávila, M.R., Souza, C.G.M., Mandarino, J.M.G., Bazo, G.L. and Cabral, Y.C.F. 2012. Physiological quality, content and activity of antioxidants in soybean seeds artificially aged. Revista Brasileira de Sementes, 34(3): 397-407. (Journal)
Baek, J.S. and Chung, N.J. 2012. Seed wintering and deterioration characteristics between weedy and cultivated rice. Rice, 5(1): 21-32. (Journal)
Balešević-Tubić, S., Malenèiæ, D., Tatiæ, M. and Miladinovic, J. 2005. Influence of aging process on biochemical changes in sunflower seed. HELIA, 28(42): 107-114. (Journal)
Balešević-Tubić, S., Tatić, M., Miladinović, J. and Pucarević, M. 2007. Changes of fatty acids content and vigor of sunflower seed during natural aging. Helia, 30(47): 61-67. (Journal)
Balouchi, H. and Ostadian Bidgoly, R. 2017. Effect of Seed Deterioration on Physiological and Biochemical Traits of Oil Flax (Linum usitatissimum L. Norman var.) Seed. Iranian Journal of Plant Production, 40(2): 37-53. (In Persian with English Abstract) (Journal)
Balouchi, H., Bagheri, F., kayed-nezami, R., Movahedi-Dehnavi, M. and Yadavi, A. 2014. Effect of seed aging on germination and seedling growth indices in three cultivars of Brassica napus L. Iranian Journal of Plant Researches, 26: 397-411. (In Persian) (Journal)
Barros, M., Fleuri, L.F. and Macedo, G.A. 2010. Seed lipases: sources, applications and properties - a review. Brazilian Journal of Chemical Engineering, 27(1): 15–29. (Journal)
Bates, L.S., Waldran, R.P. and Teare, I.D. 1973. Rapid determination of free proline for water studies. Plant Soil, 39: 205–208. (Journal)
Bhuiyan, M.A.R., Hoque, M.Z. and Hossain, S.J. 2009. Free radical scavenging activities of Zizyphus mauritiana. Electrical Journal of Environmental, Agriculture and Food Chemistry, 5(3): 318-322. (Journal)
Buege, J.A. and Aust, S.D. 1978. Microsomal lipid peroxidation. Methods in Enzymology, 52(C): 302-310. (Journal)
Chang, C.C., Yang, M.H., Wen, H.M. and Chern, J.C. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3): 178-182. (Journal)
Copeland, L.O. and McDonald, M.B. 2001. Seed Storage and Deterioration. Principles of Seed Science and Technology, 192–230. (Journal)
De Pinto, M.C. and De Gara, L. 2005. Changes in the ascorbate metabolism of apoplastic and symplastic spaces are associated with cell differentiation. Journal of Experimental Botany, 55(408): 2559-2569. (Journal)
Fessel, S.A., Vieira, R.D., Cruz, M.C.P., Paula, R.C. and Panobianco, M. 2006. Electrical conductivity testing of corn seeds as influenced by temperature and period of storage. Pesquisa Agropecuária Brasileira, 41(10): 1551-1559. (Journal)
Food and Agriculture Organization. 2019. Statistics: FAOSTAT agriculture. Retrieved January 10, 2021. from http://www.fao.org/faostat/en/#data/QC/visualize
Fotouo, M.H., Vorster, J., Du Toit, E.S. and Robbertse, P.J. 2020. The effect of natural long-term packaging methods on antioxidant components and malondialdehyde content and seed viability Moringa oleifera oil seed. South African Journal of Botany, 129: 17-24. (Journal)
Ghotbzadeh Kermani, S., Saeidi, G. and Sabzalian, M. 2019. Drought stress influenced sesamin and sesamolin content and polyphenolic components in sesame (Sesamum indicum L.) populations with contrasting seed coat colors. Food Chemistry, 289: 360-368. (Journal)
Gusta, L.V., Johnson, E.N., Nesbitt, N.T. and Kirkland, K.J. 2014. Effect of seeding date on canola seed quality and seed vigour. Canadian Journal of Plant Science, 84(2): 463-471. (Journal)
Hampton, J.G. and Tekrony., D.M. 1995. Handbook of vigour test methods: ISTA. 1179. (Book)
Hayat, S., Hayat, Q., Alyemeni, M.N., Wani, A.S., Pichtel, J. and Ahmad, A. 2012. Role of proline under changing environments. Plant Signaling and Behavior, 7(11): 1456–1466. (Journal)
He, X.T., Mulvaney, R.L. and Banwart, W.L. 1990. A Rapid Method for Total Nitrogen Analysis Using Microwave Digestion. Soil Science Society of America Journal, 54(6): 1625-1629. (Journal)
Heath, R.L. and Packer, L., 1986. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 189–198. (Journal)
Hoed, V.V. 2010. Phenolic compounds in seed oils. Lipid Technology, 22(11): 247-249. (Journal)
Kapoor, N., Arya, A., Siddiqui, M.A., Amir, A. and Kumar, H. 2010. Seed Deterioration in Chickpea (Cicer arietinum L.) under Accelerated Ageing. Asian Journal of Plant Sciences, 9(3): 158-162. (Journal)
Kapoor, N., Arya, A., Siddiqui, M.A., Kumar, H. and Amir, A. 2011. Physiological and biochemical change during seed deterioration of aged seeds of rice. American Journal of Plant Physiology, 6(1): 35-28. (Journal)
Khan, A., Ali, M., Siddiqui, S.U., Jatoi, S.A., Jan, S.A., Khan. N. and Ghafoor, A. 2017. Effect of Various Temperatures and Duration on Deterioration of Rice Seeds. Science Technology and Development, 36(2): 79-83. (Journal)
Kubala, S., Wojtyla, Ł., Quinet, M., Lechowska, K., Lutts, S. and Garnczarska, M. 2015. Enhanced expression of the proline synthesis gene P5CSA in relation to seed osmopriming improvement of Brassica napus germination under salinity stress. Journal of Plant Physiology, 183: 1–12. (Journal)
Lee, J., Welti, R., Roth, M., Schapaugh, W.T., Li, J. and Trick, H.N. 2011. Enhanced seed viability and lipid compositional changes during natural ageing by suppressing phospholipase Dα in soybean seed. Plant Biotechnology Journal, 10(2): 164–173. (Journal)
Maguire, J.D. 1962. Speed of germination – Aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2: 176-177. (Journal)
Mariotti, F., Tomé, D. and Mirand, P.P. 2008. Converting Nitrogen into Protein—Beyond 6.25 and Jones’ Factors. Critical Reviews in Food Science and Nutrition, 48(2): 177–184. (Journal)
Mira, S., Estrelles, E., González-Benito, M.E. and Corbineau, F. 2011. Biochemical changes induced in seeds of Brassicaceae wild species during ageing. Acta Physiologiae Plantarum, 33(5): 1803–1809. (Journal)
Mohamed, A.I.M., Abdel-Tawab, Y.M. and El-Dessouky, S.E.I. 2010. Evaluation of sesame, peanut and canola seed quality using accelerated ageing. Seed science and Boitecnology, 4(1): 52-58. (Journal)
Mohammadzadeh, J. 2015. Investigation of the effect of enzymatic and solvent extraction methods on the quality characteristics of oil and protein of canola. Iranian of Journal Oilseed Plants, 4(1): 24-32. (In Persian) (Journal)
Naikoo, M.I., Dar, M.I., Raghib, F., Jaleel, H., Ahmad, B., Raina, A. and Naushin, F. 2019. Role and Regulation of Plants Phenolics in Abiotic Stress Tolerance. Plant Signaling Molecules, 157–168. (Book)
Nelson, K.A., and Labuza, T.P. 1992. Relationship Between Water and Lipid Oxidation Rates. Lipid Oxidation in Food, 93–103. (Book)
Önder, S., Tonguç, M., Guvercin, D. and Karakurt, Y. 2020. Biochemical changes stimulated by accelerated aging in safflower seeds (Carthamus tinctorius L.). Journal of Seed Science, 42: e202042015. (Journal)
Oskouei, B. and Sheidaei, S. 2017. Seed deterioration. Iranian Journal of Seed Science and Research, 4(3): 125-143. (In Persian with English Abstract) (Journal)
Oskouei, B., Divsalar, M., Yari, L. and zareyan, A. 2012. Effect of packaging type on seed vigor of three varieties of canola stored in Qom. Iranian Journal of Seed Science Technolgy. 2(1): 13-24. (In Persian) (Journal)
Paravar, A., Maleki Farahani, S. and Gholami, S. 2019. Effect of seed deterioration on germination, biochemical and enzymatic activity of buckwheat. Iraninan Journal of Agroecology, 15(2): 49 – 57. (In Persian) (Journal)
Rajjou, L., Lovigny, Y., Groot, S.P.C., Belghazi, M., Job, C. and Job, D. 2008. Proteome-Wide Characterization of Seed Aging in Arabidopsis: A Comparison between Artificial and Natural Aging Protocols. Plant Physiology, 148(1): 620–641. (Journal)
Rezayian, M., Niknam, V. and Ebrahimzadeh, H. 2018. Penconazole and calcium improves drought stress tolerance and oil quality in canola. Soil Science and Plant Nutrition, 64(5): 1-10. (Journal)
Santos Sánchez, N.F., Salas-Coronado, R., Villanueva-Cañongo. C. and Hernandez-Carlos, B. 2019. Antioxidant Compounds and Their Antioxidant Mechanism, Antioxidants. (Book)
Shaaban, M. 2016. Effect of aging on enzymatic and non-enzymatic antioxidant changesand biochemical characteristicsin barley (Hordeum vulgare L.) seeds cv. Valfajr. Iranian Journal of Seed Science and Research, 3(3): 89-103. (In Persian) (Journal)
Shaban, M. 2013. Review on physiological aspects of seed deterioration. International Journal of Agriculture and Crop Sciences, 6: 627-631. (Journal)
Shaban, M., Ghaderifard, F., Sadeghipour, H.R. and Yamch, A. 2018. .Evaluation of germination and key enzymatic and non-enzymatic antioxidants involved in chickpea seed ageing during natural storage and accelerate ageing. Iranian Journal of crop production, 11(1): 51-71. (In Persian) (Journal)
Simić, A., Sredojević, S., Todorović, M., Đukanović, L. and Radenović, C. 2004. Studies on the relationship between the content of total phenolics in exudates and germination ability of maize seed during accelerated aging. Seed Science and Technology, 32(1): 213-218. (Journal)
Singleton, V.L., Orthofer, R. and Lamuela-Raventós, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 152–178. (Journal)
Soltani, M., Moradi, A., Tavakol Afshari, R. and Balouchi, H. 2016. Effect of different storage conditions on germination and some biochemical characteristics of castor bean (Ricinus communis) seeds. Iranian Journal of Field Crop Science, 48(1): 91-105. (In Persian with English Abstract) (Journal)
Tabatabaei, S.A. 2013. Changes in proline, protein, catalase and germination characteristics of barley seeds under salinity stress. International Research Journal of Applied and Basic Sciences, 5(10): 1266-1271. (Journal)
Tahmasbi, B., Ghaderi-Far, F., Sadeghipour, H.R. and Galeshi, S. 2015. The effect of accelerated deterioration on germination parameters, fatty acids and lipid hydroperoxides of sunflower seeds (Helianthus annuus L.). Iranian Journal of Plant Process and Function, 4(12): 73-84. (In Persian) (Journal)
Tian, P.P., Lv, Y.Y., Yuan, W.J., Zhang, S.B. and Hu, Y.S. 2019. Effect of artificial aging on wheat quality deterioration during storage. Journal of Stored Products Research, 80: 50–56. (Journal)
Verbruggen, N. and Hermans, C. 2008. Proline accumulation in plants: a review. Amino Acids, 359(4): 753–759. (Journal)
Voelker, T. and Kinney, A.J. 2001. Varitions in the biosynthesis of seed storage lipids. Annual Review of Plant Physiology and Plant Molecular Biology, 52(1): 335–361. (Journal)
Walters, C. 1998. Understanding the mechanisms and kinetics of seed ageing. Seed Science Research, 8: 223-244. (Journal)
Walters, C., Ballesteros, D., Veronica, A. and Vertucci, V.A. 2010. Structural mechanics of seed deterioration: Standing the test of time. Plant Science, 179: 565–573. (Journal)
Wu, X., Ning, F., Hu, X. and Wang, W. 2017. Genetic Modification for Improving Seed Vigor Is Transitioning from Model Plants to Crop Plants. Frontiers in Plant Science, 8(8): 1-7. (Journal)