Evaluation of germination, morphphysiological and antioxidant enzymes of barley cultivars in tolerance to natural salinity

Document Type : Research Paper


1 PhD student in Plant Physiology, Faculty of Agricultural Sciences, Shahed University, Tehran, Iran

2 Associate Professor and member of the faculty, Department of Agriculture and Plant Breeding, Faculty of Agricultural Sciences, Shahed University, Tehran Iran

3 Master of Agroacology, Faculty of Agricultural Sciences, Shahed University, Tehran, Iran

4 Department of Plant Production Engineering, Faculty of Agriculture, Technical and Vocational University of Khorasan Razavi Province, Khorasan, Iran


Identification and development of salinity cultivation cultivars, one of the ways to improve performance. The development of salt-tolerant cultivars is a potentially effective approach for minimizing yield losses.In order to investigate the effects of salinity on the qualitative and quantitative traits of rainfed and irrigated barley cultivars, this experiment was conducted in Crop Physiology Laboratory of Shahed University in 2019. Experimental factors included barley cultivars (Reyhan, Jolgeh, Khorram and Izeh) in response to four salinity levels (no salinity as control and salinity of 5, 10, 15 and 20 ds/m with Qom lake salt).The experiment was conducted as a factorial experiment in a completely randomized design with three replications. The measured traits included germination indices, photosynthetic pigments, soluble compounds and two antioxidant enzymes. Results showed that the highest germination components such as germination percentage (79.71%), germination rate (5.18 seeds per day), root length (6.55 cm), stem length (8.46 cm), longitudinal index (70.1) and the weight of the seedlings (165.17) in the absence of salinity stress.. With increasing salinity levels, RWC, membrane stability index and protein content of cultivars decreased and the content of carotenoid, proline, MDA and antioxidant enzymes superoxide dismutase and ascorbate peroxidase were highest in salinity. Among the studied barley cultivars, Khorram had the highest percentage and rate of germination as well as soluble compounds at the highest salinity level. This cultivar appears to have significant germination and growth under salinity stress conditions. In the later stages it is possible to recommend flat and Izeh cultivars.


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