Comparison of germination indices, morpho-physiological and biochemical traits of seeds and seedlings of guar and chickpea under the application of different concentrations of zinc and iron nanoparticles

Document Type : Research Paper


1 Assistant Professor, Department of Agronomy and Plant Breeding, Agricultural Faculty of Sarayan, University of Birjand, Birjand, Iran

2 Master of Agriculture and Plant Breeding, Agricultural Faculty of University of Birjand, Birjand, Iran


In order to investigate the effects of nanoparticles and their concentrations on the stages of germination, growth and development of crop plants, experiments were performed using a factorial experiment based on the completely randomized design on guar and chickpea seeds as the Petri dish and pot. In the present study, the effects of different concentrations of zinc and iron nanoparticles (control, 100, 200, and 300 mg.l-1) were examined on seed germination indices in petri dish cultivation and growth, physiological and biochemical traits of the mentioned plants in pot cultivation. The results of the Petri dish experiment showed that the highest percentage of germination, root resistance, and the lowest T50 were obtained under the interaction of chickpeas, iron nanoparticles, 300 mg.l-1. Also, the highest germination rate was obtained for guar and chickpea seeds at a concentration of 300 mg.l-1 of the iron nanoparticle. The results of the pot experiment also showed that the maximum shoot length, dry weight of aerial part and root were achieved for the plant exposed to a concentration of 300 mg.l-1 of zinc nanoparticle. Also, the content of chlorophyll a, total chlorophyll and leaf protein increased by 300 mg.l-1 of zinc nanoparticle. On the other hand, the amount of chlorophyll b and carotenoids increased under a concentration of 300 mg.l-1 of the iron nanoparticle. In general, it is concluded that zinc and iron nanoparticles in the range of concentrations of 100-300 mg.l-1 had positive effects on germination, growth, physiological and biochemical indices of seeds and seedlings of guar and chickpea in these conditions.


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