Comparison of germination indices, morpho-physiological and biochemical traits of seeds and seedlings of guar and chickpea under the application of different concentrations of zinc and iron nanoparticles

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Agronomy and Plant Breeding, Agricultural Faculty of Sarayan, University of Birjand, Birjand, Iran

2 Master of Agriculture and Plant Breeding, Agricultural Faculty of University of Birjand, Birjand, Iran

Abstract

In order to investigate the effects of nanoparticles and their concentrations on the stages of germination, growth and development of crop plants, experiments were performed using a factorial experiment based on the completely randomized design on guar and chickpea seeds as the Petri dish and pot. In the present study, the effects of different concentrations of zinc and iron nanoparticles (control, 100, 200, and 300 mg.l-1) were examined on seed germination indices in petri dish cultivation and growth, physiological and biochemical traits of the mentioned plants in pot cultivation. The results of the Petri dish experiment showed that the highest percentage of germination, root resistance, and the lowest T50 were obtained under the interaction of chickpeas, iron nanoparticles, 300 mg.l-1. Also, the highest germination rate was obtained for guar and chickpea seeds at a concentration of 300 mg.l-1 of the iron nanoparticle. The results of the pot experiment also showed that the maximum shoot length, dry weight of aerial part and root were achieved for the plant exposed to a concentration of 300 mg.l-1 of zinc nanoparticle. Also, the content of chlorophyll a, total chlorophyll and leaf protein increased by 300 mg.l-1 of zinc nanoparticle. On the other hand, the amount of chlorophyll b and carotenoids increased under a concentration of 300 mg.l-1 of the iron nanoparticle. In general, it is concluded that zinc and iron nanoparticles in the range of concentrations of 100-300 mg.l-1 had positive effects on germination, growth, physiological and biochemical indices of seeds and seedlings of guar and chickpea in these conditions.

Keywords


Amiri, S.R., Parsa, M., Bannayan Aval, M., Nassiri Mahallati, M. and Deihimfard, R. 2015. Effect of irrigation and nitrogen fertilizer levels on yield and yield components of chickpea (Cicer arietinum L.) under Mashhad climatic conditions. Iranian Journal of Pulses Research, 6(1): 66-77. (In Persian)(Journal)
Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Polyphenol-oxidase in Beta vulgaris. Plant Physiology, 24: 1-15. (Journal)
Aslani, F., Bagheri, S., Julkapli, N.M., Juraimi, A.S., Hashemi, F.S. and Baghdadi, A. 2014. Effects of engineered nanomaterials on plants growth. The Scientific World Journal, 10: 1-29. (Journal)
Barrena, R., Casals, E., Colon, J., Font, X., Sanchez, A. and Puntes, V. 2009. Evaluation of the ecotoxicity of model nanoparticles. Chemosphere, 75: 850-857. (Journal)
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Plant Physiology, 72: 248-254. (Journal)
Burman, U., Saini, M. and Kumar, P. 2013. Effect of zinc oxide nanoparticles on growth and antioxidant system of chickpea seedlings. Toxicological and Environmental Chemistry, 95(4): 605-612. (Journal)
Canas, J.E., Long, M.Q., Nations, S., Vadan, R., Dai, L. and Luo, M.X. 2008. Effects of functionalized and nonfunctionalized singlewalled carbon nanotubes on root elongation of select crop species. Environmental Toxicology and Chemistry, 27: 1922-1931. (Journal)
Dastborhan, S., Ghassemi-Golezani, K. and Yeganehpoor, F. 2019. Changes in germination and growth indices of borage (Borago officinalis L.) in response to seed priming and different irrigation intervals. Iranian Journal of Seed Science and Research, 6(1): 1-18. (In Persian)(Journal)
Daughton, C.G. and Ternes, T.A. 1999. Pharmaceuticals and personal care products in the environment: agents of subtle change.  Environmental Health Perspectives, 107: 907–938. (Journal)
Davis, J.G., Hossner, L.R. and Persaud, N. 1993. Elemental toxicity effects on the germination and growth of pearl millet seedlings. Journal of Plant Nutrition, 16: 1957-1968. (Journal)
El-Temsah, Y.S. and Joner, E.J. 2012. Impact of Fe and Ag nanoparticles on seed germination and differences in bioavailability during exposure in aqueous suspension and soil. Environmental Toxicology, 27: 42-49. (Journal)
Eskandarinasab, M., Rafieiolhossaini, M., Roshandel, P. and Tadayon, M.R. 2019. Investigation of Seed Germination Indices and Anthocyanin Content of Niger (Guizotia abyssinica) Seedling under the Effect of Three Nanoparticles. Iranian Journal of Seed Research, 5(2): 73-89. (In Persian)(Journal)
Esparham, E., Saeidisar, S., Mahmoodzadeh, H. and Hadi, M.R. 2017. The Effects of Zinc Oxide (ZnO) Nanoparticles on the Germination, Biochemical and Ultrastructural Cell Characteristics of Ricinus communis. Journal of Cell and Tissue, 8(2): 151-165. (In Persian)(Journal)
Fediuc, E. and Laszlo Erdei, A. 2002. Physiological and biochemical aspects of cadmium toxicity and protective mechanisms induced in phragmites Australia and Typha latifolia. Plant Physiology, 5: 129-132. (Journal)
Ganjeali, A., Joveynipour, S., Porsa, H. and Bagheri, A. 2011. Selection for drought tolerance in Kabuli chickpea genotypes in Nishabur region. Iranian Journal of Pulses Research, 2: 27-38. (In Persian)(Journal)
Gao, F.Q., Hong, F.S., Liu, C., Zheng, L., Su, M.Y., Wu, X., Yang, F., Wu, C. and Yang, P. 2006. Mechanism of nanoanatase TiO2 on promoting photosynthetic carbon reaction of spinach: inducing complex of Rubisco–Rubisco activase. Biological Trace Element Research, 11: 239-254. (Journal)
Hediat, M. and Salama, H. 2012. Effect of cilver nanoparticles in some crop plants, common bean (Phaseolus vulgaris L.) and corn (Zea mays L.). Research Journal of Biotechnology, 3(10):190-197. (Journal)
Hirt, H., Casari, G. and Barta, A. 1989. Cadmium echanced gene expression in suspension culture cells of tobacco. Planta, 179: 414-426. (Journal)
 
Hong, F., Zhou, J., Liu, C., Yang, F., Wu, C., Zheng, L. and Yang, P. 2005. Effect of nano-TiO2 on photochemical reaction of chloroplasts of spinach. Biological Trace Element Research, 105: 269-279. (Journal)
Kafi, M., Zand, A., Kamkar, B., Abbasi, F., Mahdavi Damghani, M. and Sharifi, H.R. 2008. Plant physiology (4th Ed.). Jahad Daneshgahi Mashhad Press. (In Persian)(Book)
Kapustka, L.A. and Reporter, M. 1993. Terrestrial Primary Producers. In: Ecotoxicology. Blackwell Scientific Publications (Ed.p.Calow), London. (Book)
Khodakovskaya, M., Dervishi, E., Mahmood, M., Xu, Y., Li, Z., Watanabe, F. and Biris, A.S. 2009. Carbon nanotubes are able to penetrate plant seed coat and dramtically affect seed germination and plant growth. American Chemical Society, 3(10): 3221-3227. (Journal)
Lichtenthaler, H. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods of Enzymology, 148: 350-382. (Journal)
Lin, D. and Xing, B. 2007. Phytotoxicity of nanoparticles: Inhibition of seed germination and root growth. Environmental Pollution, 20: 1-8. (Journal)
Loggale, L.B. 2018. Responses of guar to supplemental irrigation in heavy clay soils of Abu Naama. IOSR Journal of Agriculture and Veterinary Science; 11(9): 12-16. (Journal)
Ma, X., Geiser-Lee, J., Deng, Y. and Kolmakov, A. 2010. Interactions between engineered nanoparticles (ENPs) and plants: phytotoxicity, uptake and accumulation. Science of the Total Environment, 408(16): 3053-3061. (Journal)
Mahajan, P., Dhoke, S.K. and Khanna, A.S. 2011. Effect of Nano-ZnO particle suspension on growth of mung (Vigna radiate) and gram (Cicer arietinum) seedlings using plant agar method. Journal of Nanotechnology, 2011: 1-7. (Journal)
Mahdi Nezhad, N., Mousavi, H., Fakheri, B. and Heidari, F. 2019. The assesment of the effects of the nanoparticles on some physiological traits changes, photosynthetic pigments and the prthenolide of chamomile plant (Tanacetum parthenium) under Water dificit stress. Journal of Plant Process and Function, 8 (29): 219-227. (Journal)
Mahmoodzadeh, H. and Aghili, R. 2014. Effect on germination and early growth characteristics in wheat plants (Triticum aestivum L.) seeds exposed to TiO2 nanoparticles. Journal of Chemical Health Risks, 4(1): 29-36. (Journal)
Mansouri Gandomani, V., Omidi, H. and Bostani, A.A. 2019. Study on effects of pretreatment nanoparticle silicon dioxide (SiO2) on seed germination and biochemical indicate of soybean (Glycine max L.) cultivars Williams under salinity. Iranian Journal of Seed Science and Research, 6(3): 299-315. (In Persian)(Journal)
Mazaheri Tirani, M., Madadkar Haghjou, M. and Ismaili, A. 2019. Effect of bulk and nano zinc oxide on seed germination and growth indices in tobacco (Nicotiana tabacum L.) seedlings. Iranian Journal of Seed Science and Research, 6(3): 369-380. (In Persian)(Journal)
Mohamadipoor, R., Sedaghathoor, Sh. and Mahboub-Khomami, A. 2013. Effect of application of iron fertilizers in two methods 'foliar and soil application' on growth characteristics of Spathyphyllum illusion. European Journal of Experimental Biology, 3: 232-240. (Journal)
Monica, C.R. and Cremonini, R. 2009. Nanoparticles and higher plants. Cariologia, 62: 161-165. (Journal)
Racuciu, M. and Creanga, D. 2007. TMA-OH coated magnetic nanoparticles internalized in vegetal tissue. Romanian Journal of Physics, 52: 395-402. (Journal)
Rout, G.R. and Das, P. 2002. Rapid hydroponic screening for molybdenum tolerance in rice through morphological and biochemical analysis. Rostlinna Vyroba, 48: 505–512. (Journal)
Sayedena, S.V., Pilehvar, B., Abrari-vajari, K., Zarafshar, M. and Eisvand, H.R. 2019. Effects of TiO2 Nanoparticles on Germination and Primary Growth of Mountain Ash (Sorbus luristanica). Iranian Journal of Seed Research, 6(1): 173-184. (In Persian)(Journal)
Tadayon, M.R. and Norouzi, S. 2015. Effect of nano titanium oxide, nano zinc and multiwall carbon nano tube on yield and yield components of green gram (vigna radiate L.). Journal of Crop Improvement, 17(1): 169-182. (In Persian)(Journal)
Tadayon, M.R., Falah, S., Fadaei Tehrani, A.A and Norouzi, S. 2013. Effects of multi wall carbon nanotube and nanosilver on some ‎physiological and morphological traits of faba bean (Vicia faba L.)‎. Journal of Plant Process and Function, 2(5): 61-72. (In Persian)(Journal)
 
Van Dongen, J.T., Ammerlaan, A.M.H., Wouterlood, M., Van Aelst, A.C.V. and Borstlap, A.C. 2003. Structure of the developing pea seed coat and the post-phloem transport pathway of nutrients. Annals of Botany, 91: 729-737. (Journal)
Xiong, Z.T. 1998. Lead uptake and effects on seed germination land plant growth in a Pb hyperaccumulator Brassica pekinensis Rupr. Bulletin Environmental Contamination and Toxicology, 60: 285-291. (Journal)
Yang, F., Hong, F., You, W., Liu, C., Gao, F., Wu, C. and Yang, P. 2006. Influence of nano-anatase TiO2 on the nitrogen metabolism of growing spinach. Biological Trace Element Research, 110: 179-190. (Journal)
Yazdani Biuki, R.R., Rezvani Moghaddam, P., Khazaie, H.R., Ghorbani, R. and Astaraei, A.R. 2011. Effects of salinity and drought stresses on germination characteristics of milk thistle (Silybum marianum). Iranian Journal of Field Crops Research, 8(1): 12-19. (In Persian)(Journal)
Zheng, L. Hong, F., Lu, S. and Liu, C. 2005. Effect of nano-TiO2 on strength of naturally aged seeds and growth of spinach. Biological Trace Element Research, 104: 83-91. (Journal)