Proteome analysis of wheat seeds under severe drought stress and recovery

Document Type : Research Paper


1 Research Assistant Prof. Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran

2 Researcher, Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran


Seedling wheat spirit are tolerant to strict dehydration up to the 4th day following imbibitions. The aim of present study is investigation of responsive proteins to drought stress and recovery in seeds attached to four day seedlings. The test was done by drought stress using interruption of irrigation in two time points (10 and 20 days) and recovery for 3 and 7 days in a growth chamber. All the experiments were conducted based on Completely Randomized Design (CRD) with three replications.Seedlings attached to seedlings were examined for physiological and protein assays. Physiological traits showed strict drought stress level on seed and recovery could return seedlings to normal condition. 2DE done on seed of all treatments and replicates and 657 protein spots were reproducibly detected by Ag Nostaining from seeds. Finally, after significance test, 130 spots were detected by searching protein data banks. Drought stress has increased the expression of genes involved in the production of defense proteins (proline increase), the breakdown ofseed storage compounds, and the production of proteins that are effective in structural changes in the cell wall. On the other hand, reduced expression of proteins effective in respiration and protein breakdown has reduced energy consumption in cells under stress. Under re-irrigation conditions, increased expression of proteins effective in carbohydrate metabolism has led to the consumption of nutrients in seeds (reduction of seed weight) and the production of new structural proteins (presence of new leaves and roots).


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