Changes in spring wheat defense system using zinc and biofortified seeds with this elementunder drought stress

Document Type : Research Paper

Authors

1 Assistant Professor of Plant Production and Genetics Department, Faculty of Agriculture, Maragheh University, Maragheh, Iran

2 M.Sc. Graduate, Plant Production and Genetics Department, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

3 Professor of Plant Production and Genetics Department, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

Drought stress and zinc deficiency have always been considered as problems affecting the yield of cereals, especially wheat. In order to influence of zinc application and and its internal amount on growth and defense system in different moisture conditions, a factorial research was conducted based on a completely randomized design at Ardabil University and its biochemical parameters were measured at Maragheh University in 2017. Factors studied included biofortified seeds, application of zinc and drought stress. The results of this study showed that the three-way interaction on stem height, leaf area, chlorophyll, superoxide dismutase activity, Cu/Zn-SOD isozyme, ascorbate peroxidase, catalase and hydrogen peroxide, malondialdehyde, proline was significant at 1 and 5% probability levels. According to the mean comparisons, the highest activity of superoxide dismutase, Cu/Zn-SOD isozyme, ascorbate peroxidase and catalase in 30% of field capacity was recorded in the treatment of increased seeds by soil method. The result can be seen in modulating the amount of hydrogen peroxide and malondialdehyde in this treatment. Also, based on these results, the amount of chlorophyll, leaf area and plant height in this treatment showed a significant increase compared to the treatment of control seed application in irrigation conditions of 30% of field capacity. As a final result, it can be acknowledged that the application of soil-biofortified seeds in the Registered Seed fields along with the application of 25 kg/ha of sulfate zinc in the main fields in different moisture conditions, can be introduced as a suitable treatment for field research.

Keywords


Abbasi, A. and Enayati, W. 2013. Decrease of cell defense mechanisms efficiency and oxidative stress accruing in lake of Mg condition. Iranian Journal of Dryland Agriculture, 1, 4: 41-52. (In Persian)(Journal)
Abbasi, A., Shekari, F., Mosavi, S.B., Sabaghnia, N. and Javanmard, A. 2017. Effect of zinc sulfate in
 
the quantity and quality of wheat grain under soil zinc deficiency and drought stress. Cereal Research, 7(1): 1-18. (In Persian). (Journal)
Abbasi, A., Shekari, F., Mosavi, S.B., Sabaghnia, N., and Javanmard, A. 2016. The Partitioning Trend of Resources and Alpha-Amylase Enzyme Activity with Zinc Priming in Wheat (Triticum aestivum L.) Seed. Iranian Journal of Seed Research. 3, 2: 1-13. (In Persian)(Journal)
Abi, H. 1984. Catalase in vitro. Method of Enzymology, 105:121-126.
Ali Ehyaee, M, and Behbahanizadeh, A.A. 1993. Describe the Methods of Soil Analysis. Soil and Water Research Institute, No. 893. (In Persian)(Book)
Alloway, B.J. 2008. Zinc in Soils and Crop Nutrition: Second edition, published by IZA and_IFA, Brussels, Belgium and Paris, France. (Book)
Alloway, B.J. 2009. Soil factors associated with zinc deficiency in crops and humans. Environmental Geochemistry and Health, 31(5), 537-548. (Journal)
Alscher, R.G., Erturk, N. and Heath, L.S., 2002. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. Journal of experimental botany, 53(372): 1331-1341. (Journal)
Amiri, A., Baninasab, B., Ghobadi, C. and Khoshgoftarmanesh, A.H. 2016. Zinc soil application enhances photosynthetic capacity and antioxidant enzyme activities in almond seedlings affected by salinity stress. Photosynthetica, 54(2): 267-274. (Journal)
Arnon D.T. 1949. Copper enzymes in isolation chloroplast phenoloxidase in Beta vulgaris. Plant Physiology, 24: 1-15. (Journal)
Arora, Ajay, R.K. Sairam, and G.C. Srivastava. 2002. Oxidative stress and antioxidative system in plants. Current science, 1227-1238. (Journal)
Asad, A. and Rafique, R. 2000. Effect of zinc, copper, iron, manganese and boron on the yield and yield components of wheat crop in Tehsil Peshawar. Pakistan Journal of Biological Sciences, 3(10): 1615-1620. (Journal)
Ayala, A., Muñoz, M.F. and Argüelles, S. 2014. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative medicine and cellular longevity, 2014. (Journal)
Bailey-Serres, J., and Mittler, R. 2006. The roles of Reactive Oxygen Species in Plant Cells. (Book)
Bates, L.S., Waldren, R.P. and Teare, I.D. 1973. Rapid determination of free proline for water-stress studies. Plant and soil, 39(1), pp.205-207. (Journal)
Blokhina, O. and Fagerstedt, K.V. 2010. Oxidative metabolism, ROS and NO under oxygen deprivation. Plant Physiology and Biochemistry, 48(5): 359-373. (Journal)
Cakir R. 2004. Effect of water stress at different development stages on vegetative and reproductive growth of corn. Field Crops Research, 89: 1-16. (Journal)
Cakmak, I. 2008. Enrichment of cereal grains with zinc: agronomic or genetic biofortification? Plant and soil, 302(1-2):1-17. (Journal)
Cakmak, I., 2012. Harvest Plus zinc fertilizer project: Harvest Zinc. Better Crops, 96(2): 17-19. (Journal)
Cakmak, I., Pfeiffer, W.H. and McClafferty, B., 2010. Biofortification of durum wheat with zinc and iron. Cereal chemistry, 87(1): 10-20. (Journal)
Candan, N., Cakmak, I., and Ozturk, L. 2018. Zinc biofortified seeds improved seedling growth under zinc deficiency and drought stress in durum wheat. Journal of Plant Nutrition and Soil Science, 181(3): 388-395. (Journal)
Castillo-González, J., Ojeda-Barrios, D., Hernández-Rodríguez, A., González-Franco, A.C., Robles-Hernández, L. and López-Ochoa, G.R. 2018. Zinc metalloenzymes in plants. Interciencia, 43(4): 242-248. (Journal)
Caverzan, A., Passaia, G., Rosa, S.B., Ribeiro, C.W., Lazzarotto, F. and Margis-Pinheiro, M. 2012. Plant responses to stresses: role of ascorbate peroxidase in the antioxidant protection. Genetics and molecular biology, 35(4): 1011-1019. (Journal)
Chagas, R.M., Silveira, J.A., Ribeiro, R.V., Vitorello, V.A. and Carrer, H. 2008. Photochemical damage and comparative performance of superoxide dismutase and ascorbate peroxidase in sugarcane leaves exposed to paraquat-induced oxidative stress. Pesticide Biochemistry and Physiology, 90(3): 181-188. (Journal)
Chen, L.M., Lin, C.C. and Kao, C.H. 2000. Copper toxicity in rice seedlings: Changes in antioxidative enzyme activities, H2O2level, and cell wall peroxidase activity in roots. Botanical Bulletin of
 
Academia Sinica, 41: 99-103. (Journal)
Dos Santos, J.O., Andrade, C.A., De Souza, K.R.D., De Oliveira Santos, M., Brandão, I.R., Alves, J.D. and Santos, I.S., 2019. Impact of Zinc Stress on Biochemical and Biophysical Parameters in Coffea Arabica Seedlings. Journal of Crop Science and Biotechnology, 22(3): 253-264. (Journal)
El-Beltagi, H.S. and Mohamed, H.I., 2013. Reactive oxygen species, lipid peroxidation and antioxidative defense mechanism. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(1): 44-57. (Journal)
Fahad, S., Bajwa, A.A., Nazir, U., Anjum, S.A., Farooq, A., Zohaib, A., Sadia, S., Nasim, W., Adkins, S., Saud, S. and Ihsan, M.Z. 2017. Crop production under drought and heat stress: plant responses and management options. Frontiers in plant science, 8(1): 1147. (Journal)
Farooq, M., Wahid, A., Kobayashi, N., Fujita, D.B.S.M.A. and Basra, S.M.A. 2009. Plant Drought Stress: Effects, Mechanisms and Management. In Sustainable agriculture (pp. 153-188). Springer, Dordrecht. (Book)
Flintham, J.E., Angus, W.J. and Gale, M.D. 1997. Heterosis, over dominance for grain yield, and alpha-amylase activity in Fl hybrids between near-isogenic Rht dwarf and tall wheats. Journal of Agricultuer Science, 129:371–378. (Journal)
Foti, S., Cosentino, S.L., Patane, C. and D'agosta, G.M. 2002. Effect of osmoconditioning upon seed germination of sorghum (Sorghum bicolor (L.) Moench) under low temperatures. Seed Science and Technology, 30(3): 521-533. (Journal)
Foyer, C.H. 2018. Reactive oxygen species, oxidative signaling and the regulation of photosynthesis. Environmental and experimental botany, 154: 134-142. (Journal)
Hafeez, B., Khanif, Y.M. and Saleem, M. 2013. Role of zinc in plant nutrition-a review. Journal of Experimental Agriculture International, 374-391. (Journal)
Harris, D., Tripathi, R.S., and Joshi, A. 2002. On-farm seed priming to improve crop establishment and yield in dry direct-seeded rice. Direct seeding: Research Strategies and Opportunities, International Research Institute, Manila, Philippines, 231-240. (Conference)
Hassan, N., Irshad, S., Saddiq, M.S., Bashir, S., Khan, S., Wahid, M.A., Khan, R.R. and Yousra, M. 2019. Potential of zinc seed treatment in improving stand establishment, phenology, yield and grain biofortification of wheat. Journal of Plant Nutrition, 42(14): 1676-1692. (Journal)
Hayat, S., Hayat, Q., Alyemeni, M.N., Wani, A.S., Pichtel, J. and Ahmad, A. 2012. Role of proline under changing environments: a review. Plant signaling and behavior, 7(11):1456-1466. (Journal)
Hernandez-Ruiz, J., Arnao, M.B., Hiner, A.N., garcía-cánovas, F. and ACOSTA, M. 2001. Catalase-like activity of horseradish peroxidase: relationship to enzyme inactivation by H2O2. Biochemical Journal, 354(1):107-114. (Journal)
Imtiaz, M., Alloway, B.J., Shah, K.H., Siddiqui, S.H., Memon, M.Y., Aslam, M. and Khan, P. 2003. Zinc nutrition of wheat: I: Growth and zinc uptake. Asian Journal of Plant Sciences, 2(2): 152-155. (Journal)
Jon, C. and Loon, V. 1980. Analytical atomic absorption spectroscopy. Academic Press Inc. 5:158-220. (Journal)
Kapoor, D., Singh, S., Kumar, V., Romero, R., Prasad, R. and Singh, J. 2019. Antioxidant enzymes regulation in plants in reference to reactive oxygen species (ROS) and reactive nitrogen species (RNS). Plant Gene, 19: 100182. (Journal)
Kaur, R., Arora, S. and Thukral, A.K. 2009. Enhancing seed germination of Chlorophytum borivilianum Sant. Et Fernand. with PGRs, steroidal hormones and zinc. Research Journal of Seed Science, 2(2): 32-39. (Journal)
Kaya, Y., Kaya, Y., Arisoy, R.Z. and Göcmen, A. 2002. Variation in grain yield and quality traits of bread wheat genotypes by zinc fertilization. Pakistan Journal of Agronomy, 1(4):142-144. (Journal)
Kehrer, J.P. 2000. The Haber–Weiss reaction and mechanisms of toxicity. Toxicology, 149(1), 43-50. (Journal)
Khan, B., Baloch, M.S. and Hussain, S.M. 1999. Micro-Nutritional Studies in Pigeon pea. Sciences, 2(2): 399-401. (Journal)
 
Khan, M., Fuller, M. and Baloch, F. 2008. Effect of soil applied zinc sulphate on wheat (Triticum aestivum L.) grown on a calcareous soil in Pakistan. Cereal Research Communications, 36(4): 571-582. (Journal)
Lamaoui, M., Jemo, M., Datla, R. and Bekkaoui, F. 2018. Heat and drought stresses in crops and approaches for their mitigation. Frontiers in chemistry, 6: 26. (Journal)
Laxa, M., Liebthal, M., Telman, W., Chibani, K. and Dietz, K.J. 2019. The role of the plant antioxidant system in drought tolerance. Antioxidants, 8(4): 94. (Journal)
Li, C.R., liang, D.D., Li, J., Duan, Y.B., Li, H.A.O., Yang, Y.C., Qin, R.Y., Li, L.I., Wei, P.C. and Yang, J.B. 2013a. Unravelling mitochondrial retrograde regulation in the abiotic stress induction of rice alternative oxidase 1 genes. Plant, cell and environment, 36(4): 775-788. (Journal)
Li, X., Yang, Y., Jia, L., Chen, H. and Wei, X. 2013b. Zinc-induced oxidative damage, antioxidant enzyme response and proline metabolism in roots and leaves of wheat plants. Ecotoxicology and environmental safety, 89: 150-157. (Journal)
Li, Z., Han, X., Song, X., Zhang, Y., Jiang, J., Han, Q., Liu, M., Qiao, G. and Zhuo, R. 2017. Overexpressing the Sedum alfredii Cu/Zn superoxide dismutase increased resistance to oxidative stress in transgenic Arabidopsis. Frontiers in plant science, 8:1010. (Journal)
Lobell, D.B., Burke, M.B., Tebaldi, C., Mastrandrea, M.D., Falcon, W.P. and Naylor, R.L. 2008. Prioritizing climate change adaptation needs for food security in 2030. Science, 319: 607–610. (Journal)
Lomonte, C., Sgherri, C., Baker, A.J., Kolev, S.D. and Navari-Izzo, F. 2010. Antioxidative response of Atriplex codonocarpa to mercury. Environmental and experimental botany, 69(1): 9-16. (Journal)
Luis, A., Sandalio, L.M., Corpas, F.J., Palma, J.M., and Barroso, J.B. 2006. Reactive oxygen species and reactive nitrogen species in peroxisomes. Production, scavenging, and role in cell signaling. Plant physiology, 141(2): 330-335. (Journal)
Ma, Q.Q., Wang, W., Li, Y.H., Li, D.Q. and Zou, Q. 2006. Alleviation of photoinhibition in drought-stressed wheat (Triticum aestivum) by foliar-applied glycinebetaine. Journal of Plant Physiology, 163(2): 165-175. (Journal)
Malakouti, M.J. 2007. Zinc is a neglected element in the life cycle of plants. Middle Eastern and Russian Journal of Plant Science and Biotechnology, 1(1): 1-12. (Journal)
Mao, L., Chen, J., Peng, Q., Zhou, A. and Wang, Z. 2013. Effects of different sources and levels of zinc on H 2 O 2-induced apoptosis in IEC-6 cells. Biological trace element research, 155(1): 132-141. (Journal)
Mhamdi, A. and Van Breusegem, F. 2018. Reactive oxygen species in plant development. Development, 145(15): dev164376. (Journal)
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in plant science, 7(9): 405-410. (Journal)
Mohamadi Dizaj, H. and Shekari, F. 2016. The Effect of Zinc Sulfate on Biofortification and Morphological Changes in Spring Wheat. Water and Soil Science, 26(3):.277-292. (Journal)
Ozturk, L., Yazici, M.A., Yucel, C., Torun, A., Cekic, C., Bagci, A., Ozkan, H., Braun, H.J., Sayers, Z. and Cakmak, I. 2006. Concentration and localization of zinc during seed development and germination in wheat. Physiologia Plantarum, 128(1): 144-152. (Journal)
Ozyigit, I.I., Filiz, E., Vatansever, R., Kurtoglu, K.Y., Koc, I., Öztürk, M.X. and Anjum, N.A. 2016. Identification and comparative analysis of H2O2-scavenging enzymes (ascorbate peroxidase and glutathione peroxidase) in selected plants employing bioinformatics approaches. Frontiers in plant science, 7 (1): 301. (Journal)
Pattanagul, W. and Madore, M.A. 1999. Water deficit effects on raffinose family oligosaccharide metabolism in Coleus. Plant Physiology, 121(3): 987-993. (Journal)
Rai, V.K., 2002. Role of amino acids in plant responses to stresses. Biologia plantarum, 45(4): 481-487. (Journal).
Rashid, A., Ram, H., Zou, C.Q., Rerkasem, B., Duarte, A.P., Simunji, S., Yazici, A., Guo, S., Rizwan, M., Bal, R.S. and Wang, Z. 2019. Effect of zinc biofortified seeds on grain yield of wheat, rice, and common bean grown in six countries. Journal of Plant Nutrition and Soil Science, 182(5): 791-804. (Journal)
Ren, J., Sun, L.N., Zhang, Q.Y. and Song, X.S. 2016. Drought tolerance is correlated with the activity of antioxidant enzymes in Cerasus humilis seedlings. BioMed research international, 2016.
 
(Conference).
Rengel, Z. and Graham, R.D. 1995. Importance of seed Zn content for wheat growth on Zn-deficient soil: II. Grain yield. Plant and Soil, 173(2): 259-266. (Journal)
Rengel, Z., Römheld, V. and Marschner, H. 1998. Uptake of zinc and iron by wheat genotypes differing in tolerance to zinc deficiency. Journal of Plant Physiology, 152(4-5): 433-438. (Journal)
Roosta, H.R., Estaji, A. and Niknam, F. 2018. Effect of iron, zinc and manganese shortage-induced change on photosynthetic pigments, some osmoregulators and chlorophyll fluorescence parameters in lettuce. Photosynthetica, 56(2): 606-615. (Journal)
Saeid, M. and Zabihi-e-Mahmoodabad, R. 2009. Evaluation of drought stress on relative water content and chlorophyll content of sesame (Sesamum indicum L.) genotypes at early flowering stage. Res J Environ Sci, 3: 345-350. (Journal)
Sairam, R.K., Deshmukh, P.S. and Saxena, D.C. 1998. Role of antioxidant systems in wheat genotypes tolerance to water stress. Biologia plantarum, 41(3): 387-394. (Journal)
Sairam, R.K., Rao, K.V. and Srivastava, G.C., 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant science, 163(5): 1037-1046. (Journal)
Samreen, T., Shah, H.U., Ullah, S. and Javid, M. 2017. Zinc effect on growth rate, chlorophyll, protein and mineral contents of hydroponically grown mungbeans plant (Vigna radiata). Arabian journal of Chemistry, 10: S1802-S1807. (Journal)
Sharma, P., Jha, A.B., Dubey, R.S. and Pessarakli, M. 2012. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of botany, 2012. (Journal)
Singh. J., Padmalochan, H. and Jolly, B. 2014. Potential of Vigna unguiculata as a Phytoremediation Plant in the Remediation of Zn from Contaminated Soil. American Journal of Plant Sciences, 5: 1156-1162. (Journal)
Srivastava, P.C., Rawat, D., Pachauri, S.P. and Shukla, A.K. 2016. Seedling Zinc-Uptake in Wheat Cultivars of Varying Zinc-Use Efficiency. Journal of Crop Improvement, 30(6): 684-702. (Journal)
Stewart, R.R.C. and Bewley, J.D. 1980. Lipid peroxidation associated aging of soybean axes. Advances in Plant Physiologyogy, 65: 245-248. (Journal)
Weraduwage, S.M., Chen, J., Anozie, F.C., Morales, A., Weise, S.E. and Sharkey, T.D. 2015. The relationship between leaf area growth and biomass accumulation in Arabidopsis thaliana. Frontiers in plant science, 6(1): 167. (Journal)
Yilmaz, A., Ekiz, H., Torun, B., Gultekin, I., Karanlik, S., Bagci, S.A. and Cakmak, I. 1997. Effect of different zinc application methods on grain yield and zinc concentration in wheat cultivars grown on zinc‐deficient calcareous soils. Journal of plant nutrition, 20(4-5): 461-471. (Journal)
Yousuf, P.Y., Hakeem, K.U.R., Chandna, R. and Ahmad, P. 2012. Role of Glutathione Reductase in Plant Abiotic Stress. In Abiotic Stress Responses in Plants (pp. 149-158). Springer, New York, NY. (Book)