Grouping of some bean genotypes in various drought stress treatment in Germination Stages and the expression investigating of some genes in resistant and susceptible

Document Type : Research Paper

Authors

1 Assistant Professor. Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Associate Professor, Department of Horticultural, Faculty of Agriculture, University of Guilan, Rasht, Iran

4 MS, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan,Rasht, Iran.

Abstract

In order to study the effects of drought stress induced by polyethylene glycol 6000 on germination components of 21 bean genotypes, a randomized complete design with factorial arrangement of one drought level (-0.148 MPa) and control level (0), in 3 replication and with the purpose of determining the resistance and susceptible genotype, was conducted. Results of anova indicate that all genotypes in all studied components (root, shoot, fresh weight of root and shoot, dry weight of root and shoot, growth rate root and shoot, root to shoot length ratio, difference of fresh and dry weight root, difference of fresh and dry weight shoot)were affected significantly by drought stress. Genotype effect and also the interaction between genotype and drought, was significant for all studied components. According to results of cluster analysis with the ward method, all genotypes in normal and drought conditions, were placed into four different groups, the Ghalaf Siyah and Pak genotypes were chosen as resistance and susceptible genotypes in drought stress conditions.In this study the expression patterns of three drought induced-genes was investigated in two Ghalaf Siyah (resistant genotype) and Pak (sensitive genotype) using Real time PCR technique. After RNA extraction and cDNA synthesis, expression of three genes, including,GR, PRX and APX were studied in the seedling stage of Ghalaf Siyah and Pak genotype under drought stress for control, 72, 144, 192 and 240 hours of the treatments.The results indicated that in Ghalaf Siyah genotype the drought  induced-genes generally had higher expression than Pak cultivar.Expression of three genes PRX, GR and APX at the 240 h in the tolerant genotype, it was two, four and six more than the sensitive genotype.

Keywords

References

Achary, V., Murali, M., Reddy, C.S., Pandey, P., Islam, T., Kaul,T. and Reddy, M.K. 2015. Glutathione reductase a unique enzyme: molecular cloning, expression and biochemical characterization from the stress adapted C 4 plant, Pennisetum glaucum (L.) R. Br. Molecular Biology Reports, 42: 947-962 .(Journal)
Ahmed, S., Nawata, E., Hosokawa, M., Domae, Y. and Sakuratani, T. 2002. Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant Science, 163: 117-123. (Journal)
Aliakbari, M. and Razi, H. 2013. Isolation of Brassica napus MYC2 gene and analysis of its expression in response to water deficit stress. Molecular Biology Research Communications, 2: 63-71. (Journal)
Allen, R.D. 1995. Dissection of oxidative stress tolerance using transgenic plants. Plant physiology, 107: 1049.  (Journal)
Andalibi, B., Zangani, E., and Haghnazari, A. 2005. Effects of water stress on germination indices in six rapeseed cultivars (Brassica napus L.). Iranin Journal of Agricultural Sciences, 36: 457-463. (In Persian)(Journal)
Aono, M., Kubo, A., Saji, H., Tanaka, K. and Kondo, N. 1993. Enhanced tolerance to photooxidative stress of transgenic Nicotiana tabacum with high chloroplastic glutathione reductase activity. Plant and Cell Physiology, 34: 129-135.(Journal)
Ashraf, M. and Iram, A. 2005. Drought stress induced changes in some organic substances in nodules and other plant parts of two potential legumes differing in salt tolerance. Flora-Morphology, Distribution, Functional Ecology of Plants, 200: 535-546. (Journal)
Carvalho, L.C. and Amâncio, S. 2002. Antioxidant defence system in plantlets transferred from in vitro to ex vitro: effects of increasing light intensity and CO2 concentration. Plant Science, 162: 33-40. (Journal)
Covell, S, Ellis, R.H., Roberts, E.H. and Summerfield, R.J. 1986. The influence of temperature on seed germination rate in grain legumes: I. A comparison of chickpea, lentil, soyabean and cowpea at constant temperatures. Journal of experimental botany, 37: 705-715 .(Journal)
Dietz, K-J., Jacob, S., Oelze, M-L., Laxa, M., Tognetti, V., Nunes de Miranda, S.M., Baier, M. and Finkemeier, I., 2006. The function of peroxiredoxins in plant organelle redox metabolism. Journal of experimental botany, 57: 1697-170. (Journal)
FAOSTAT 2019. http://www.fao.org/faostat/en/#data/QCL.
Gaafar, R.M. and Seyam, M.M. 2018. Ascorbate–glutathione cycle confers salt tolerance in Egyptian lentil cultivars. Physiology and Molecular Biology of Plants, 24: 1083-1092. (Journal)
Jafari, M., Moosavi, S.S., Chaichi, M., Ahmadvand, Q. and Abdollahi, M. 2015. Evaluation of variability and heritability of effective traits on yield in bean (Phaseolus vulgaris L.) under non-stress and moisture stress conditions before pod formation. Applied Field Crops Research, 28: 59-66. (In Persian)(Journal)
Jebara, S., Jebara, M. Limam, F. and Elarbi, A.M. 2005. Changes in ascorbate peroxidase, catalase, guaiacol peroxidase and superoxide dismutase activities in common bean (Phaseolus vulgaris) nodules under salt stress. Journal of plant physiology, 162: 929-936. (Journal)
Jiang, M. and Zhang, J. 2002. Water stress‐induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up‐regulates the activities of antioxidant enzymes in maize leaves. Journal of experimental botany, 53: 2401-2410. (Journal)
Kamkar, B, Ghaffari, H. and Entesari, M. 2008. The Study of Temperature and Salinity Effects on germination Components of Canola Cultivars. Journal of Agricultural Sciences and Natural Resources, 15: 1-12. (In Persian)(Journal)
Kausar, R., Takahiro Makino, Z.H. and Komatsu, S. 2012. Characterization of ascorbate peroxidase in soybean under flooding and drought stresses. Molecular Biology Reports, 39: 10573-10579. (Journal)
Liebthal, M., Maynard, D. and Dietz, K-J. 2018. Peroxiredoxins and redox signaling in plants. Antioxidants and redox signaling, 28: 609-624 . (Journal)
Livak, K.J. and Schmittgen, T.D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods, 2(5): 402-408. (Journal)
 
Marmagne, A., Brabant, P., Thiellement, H. and Alix, K. 2010. Analysis of gene expression in resynthesized Brassica napus allotetraploids: transcriptional changes do not explain differential protein regulation. New phytologist, 186: 216-227. (Journal)
Michelet, L., Zaffagnini, M., Massot, V., Keryer, E., Vanacker, H., Miginiac-Maslow, M., Issakidis-Bourguet, E. and Lemaire, S.D. 2006. Thioredoxins, glutaredoxins, and glutathionylation: new crosstalks to explore. Photosynthesis Research, 89: 225-245. (Journal)
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7: 405-410. (Journal)
Mittler, R., Vanderauwera, S., Gollery, M. and Van Breusegem, F. 2004. Reactive oxygen gene network of plants. Trends in plant science, 9: 490-498. (Journal)
Mohsenzadeh Golfazani, M., Pasandideh Arjmand, M., Hassani Kumleh, H., Samizadeh Lahiji, H., Vahedi, R. and Ramezanzadeh Bishegahi, S. 2019. The effect of iron toxicity onsome of morphological traits, relative gene expression of G6PDH and peroxidase enzyme activity in resistant and susceptible genotypes of Rice (Oryza sativa). Cereal Research, 9: 207-220. (In Persian((Journal)
Mohsenzadeh Golfazani, M., Aghaeepour, S., Samiezadeh Lahiji, H. and Abdollahi, Sh. 2020. Studying expression of some genes associated with drought stress in rice (Oryza sativa L.). Cereal Research, 9: 299-314. (In Persian)(Journal)
Mohsenzadeh Golfazani, M., Mohammad, F., Hasani Kumleh, S.H. and Samizadeh Lahiji, H. 2016. Grouping of some canola genotypes in various drought stress treatment in Germination Stages based on multivariate statistical methods. Iranian Journal of Seed Sciences and Research, 3: 53-65. (In Persian((Journal)
Mohsenzadeh Golfazani, M., Pasandideh Arjmand, M., Kordrostami, M., Samizadeh Lahiji, H., Hassani Kumle, H. and Rezadoost, M.H. 2018. The effect of iron stress on the relative expression level of SOD, MDHR and GPX1 genes in tolerant and sensitive genotypes of Rice. Crop Biotechnology, 8: 1-13. (In Persian((Journal)
Naderi Zarnaghi, R. and Valizadeh, M. 2014. Investigation the activity of Glutathione Reductase and Ascorbate Peroxidaseenzymes in wheat genotypes under drought tension in flowering stage. Crop Physiology Journal, 6: 85-97. (In Persian((Journal)
Naderi Zarnaghi, R., Valizadeh, M. and Firoozi, S. 2014. Effect of free amino acids on the yield quality and quantity of forage varieties Gole-Sefid (Vicia panonica) in rainfed conditions. Iranian Dryland Agronomy Journal, 3: 127-139. (In Persian)(Journal)
Nezami, A., Khamadi, N., Khajehosiny, M. and Bagheri, A. 2010. Evaluation of Drought Tolerance in Cold Hardy Lentils (Lens culinaris medik.) at Germination Stage. Iranian Journal of Field Crops Research, 8: 138-146. (In Persian((Journal)
Noctor, G., Mhamdi, A., Chaouch, S., Han, Y., Neukermans, J. Marquez‐Garcia, B., Guillaume, Q. and Christine, H.F. 2012. Glutathione in plants: an integrated overview. Plant, Cell and Environment, 35: 454-484. (Journal)
Pasandide Arjmand, M., Samizadeh Lahiji, H. and Mohsenzadeh Golfazani, M. 2017. The investigation of some photorespiration genes relative expression in responseto drought stress in canola (Brassica napus). Crop Biotechnology, 7: 31-42. (In Persian((Journal)
Pasandideh Arjmand, M., Samizadeh Lahiji, H. and Mohsenzadeh Golfazani, M. 2017a. The investigation of some photorespiration genes relative expression in response to drought stress in canola (Brassica napus). Crop Biotechnology, 7: 31-42. (In Persian((Journal)
Pasandideh Arjmand, M., Samizadeh Lahiji, H. and Mohsenzadeh Golfazani, M. 2017b. The investigation of some photorespiration genes relative expression in response to drought stress in canola (Brassica napus). Crop Biotech, 7: 31-42. (In Persian ((Journal)
Pasandideh, M., Samizadeh, H. and Mohsenzadeh, M. 2018. The effect of drought stress on some morphological and physiological characters in canola seedling (Brassica napus). Iranian Journal of Seed Sciences and Research, 5: 95-108. (In Persian)(Journal)
Rahimi, Z. and Kafi, M. 2009. Effects of drought stress on germination characteristics of purslane (Portulaca oleracea L.). Environmental Stresses in Crop Sciences, 2: 87-91. (In Persian)(Journal)
Ratnayaka, H.H., Molin, W.T. and Sterling, T.M. 2003. Physiological and antioxidant responses of cotton and spurred anoda under interference and mild drought. Journal of experimental botany, 54: 2293-2305. (Journal)
 
Sani Rasti, M.S., Lahouti, M. and Ganjeali, A. 2014. Effect of drought stress on some morphophysiological traits and chlorophyll fluorescence of red bean seedlings (Phaseolus vulgaris L.). Iranian Journal of Pulses Reseach, 5: 103-116. (In Persian((Journal)
Scott, S.J., Jones, R.A. and Williams, W.A. 1984. Review of data analysis methods for seed germination 1. Crop science, 24: 1192-1199. (Journal)
Sharma, P. and Dubey, R.S. 2005. Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant growth regulation, 46: 209-22. (Journal)
Silvente, S., Sobolev A.P. and Lara, M. 2012. Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress. PLoS ONE, 7: e38554. (Journal)
Strid, Å. 1993. Alteration in expression of defence genes in Pisum sativum after exposure to supplementary ultraviolet-B radiation. Plant and Cell Physiology, 3: 953-949. (Journal)
Tsai, Y-C., Hong, C-Y., Liu, L-F. and Kao, C.H. 2005. Expression of ascorbate peroxidase and glutathione reductase in roots of rice seedlings in response to NaCl and H2O2. Journal of plant physiology, 162: 291-299. (Journal)
Vahedi, R., Mohsenzadeh, Golfazani, M., Pasandideh Arjmand, M. and Samizadeh Lahiji, H. 2019a. Investigation of relative expression of some genes related to iron-induced toxicity in two varieties of Rice (Oryza sativa). Crop Biotech, 9: 15-28. (InPersian((Journal)
Vahedi, R., Mohsenzadeh, Golfazani, M., Pasandideh Arjmand, M. and Samizadeh Lahiji, H. 2019b. Investigation of relative expression of some genes related to iron-induced toxicity in two varieties of Rice (Oryza sativa). Crop Biotechnology, 9: 15-28. 31-42. (In Persian((Journal)
Vaseghi, M.J., Chibani, K., Telman, W., Liebthal, M.F., Gerken, M., Schnitzer, H., Mueller, S.M. and Dietz, K.J. 2018. The chloroplast 2-cysteine peroxiredoxin functions as thioredoxin oxidase in redox regulation of chloroplast metabolism. Plant Biology, 7: 1-28. (Journal)
Wang, N., Qian, W., Suppanz, I.,Wei, L., Mao, B., Long, Y., Meng, J., Müller, A.E. and Jung, C. 2011. Flowering time variation in oilseed rape (Brassica napus L.) is associated with allelic variation in the FRIGIDA homologue BnaA. FRI. a. Journal of experimental botany, 62: 5641-5658. (Journal)
Xiong, L., Wang, R-G., Mao, G. and Koczan, J.M. 2006. Identification of drought tolerance determinants by genetic analysis of root response to drought stress and abscisic acid. Plant physiology, 142: 1065-1074. (Journal)
Yang, B., Srivastava, S., Deyholos, M.K. and Kav, N.N.V. 2007. Transcriptional profiling of canola (Brassica napus L.) responses to the fungal pathogen Sclerotinia sclerotiorum. Plant Science, 173: 156-171. (Journal)
Zadeh Bagheri, M., Javanmardi, Sh., Alozadeh, O. and Kamelmanesh, M.M. 2014. Effects of drought on grain yield and some physiological characteristics of red beangenotypes. Journal of Plant Ecophysiology, 6: 1-11. (In Persian((Journal)
Zhang, J., Nguyen, H.T. and Blum, A. 1999. Genetic analysis of osmotic adjustment in crop plants. Journal of experimental botany, 50: 291-302. (Journal)
Iranian Journal of Seed Sciences and Research
Volume 8, Issue 3
October 2021
Pages 209-224

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