Evalution of Arctium lappa seed dormancy breaking methods

Document Type : Research Paper

Authors

Depatment of Agronomy and Plant Breeding and tropical and subtropical plants research center, Islamic Azad University, Shoushtar branch, Shoushtar, Iran

Abstract

Arctium lappa is an important medical plant with seed dormancy problem. A study was carried out to investigate the effects of seed dormancy breaking treatments on Arctium lappa germination. Treatments were arranged in a completely randomized design (CRD) with five replication. The highest germination percentage was recorded in seeds treated with GA3 (500 ppm) +  H2SO4 75% (86%), GA3 (500 ppm) + hot water 70 0C (87%) and GA3 (500 ppm) + hot water 90 0C (84%). Seed scarification with hot water were better than H2SO4 because H2SO4  increased  abnormal seedling percentage. Highest abnormal seedling showed in H2SO4 75%, GA3 (500 ppm) + H2SO4 75% and KNO3 (1%) +  H2SO4 75 % treatments (11.7%, 11.3% and 10.9% respectively). Seed treatment with KNO3 and GA3 increas α- amylase activity and Auxin concentration in Arctium lappa seedlings and decreased Abscisic Acid content in seedlings. The maximum seedling vigor index obtaines in seeds treated with GA3 (500 ppm) + hot water 70 0C . Results indicated Arctium lappa seeds scarification with hot water 70 0C (10 min) followed by socking in GA3 (500 ppm) for 12 hour was most effective treatment for seed dormancy breaking and improved seedling growth.

Keywords

References

Abbasi, M., Heydari, M. and Rahemi, M. 2014. Effect  of seed acid scarification on Psidium guajava germination. Journal of Horticultural Science, 27: 394-399. (In Persian) (Journal)
Akhondi, M., Zare Hassanabadi, M., Amiri, M.S. and Shabani, S. 2017. The effects of mechanical and chemical treatments on dormancy breaking and seed germination indices of Myrtus communis L. Iranian Journal of Seed Science and Technology, 5: 1-7. (In Persian)(Journal)
Alboresi, A., Gestin, C., Leydecker, M.T., Bedu, M., Meyer, C. and Truong, H.N. 2005. Nitrate, a signal relieving seed dormancy in Arabidopsis. Plant, Cell and Environment, 28: 500–512. (Journal)
Bahadori, F. and Javanbakht, A. 2006. Effect of pre-treatments on seed germination and seedling growth of Bunium persicum of Semnan. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 14(3): 163-169. (In Persian)(Journal)
Batak, I., Devic, M., Giba, Z., Grubisic, D., Poff, K.L. and Konjevic, R. 2002. The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds. Seed Science Research, 12: 235-259. (Journal)
Bhardwaj, M., Kak, A., Gupta, A., Dashora, K. and Gupta,V. 2010. Effect of pre sowing treatment on germination of Arctium lappa- amedical plant of western Hymallia. Seed Science and Technology, 38: 784-786. (Journal)
Bhardwaj, R.L., Meena, R.R. and Mukherjee, S. 2005. Role of plant growth regulators in Guava (Psidium guajava)- A review. Agricultural Review, 26(4): 281-287. (Journal)
Chen, S.H., Kuo, S.H. and Chein, C.T. 2008. Roles of gibberellins and abscisic acid in dormancy and germination of red bayberry (Myrica rubra) seeds. Tree Physiology, 28: 1431–1439. (Journal)
Da Silva, E.A.A., Toorop, P.E., Van Aelst, A.C. and Hilhorst, H.W.M. 2004. Abscisic acid controls embryo growth potential and endosperm cap weakening during coffee (Coffea arabica cv. Rubi) seed germination. Planta, 220: 251–261. (Journal)
Darvishi Zidabadi, D., Jalali Javara, M., Dehghani, H., Rashidi Monfared, S. and Baghizadeh, A. 2015. The effect of different combinations of hormonal treatments on breaking seed dormancy in different ecotypes of  Black Zira (Bunium persicum). Iranian Journal of Seed Science and Research, 2: 55-67. (In Persian)(Journal)
Dehghanpour Farashah, H., Tavakkol Afshari, R., Sharifzadeh, F. and Chavoshinasab, S. 2011. Germination improvement and α-amylase and β-1,3-glucanase activity in dormant and nondormant seeds of Oregano (Origanum vulgare). Australian Journal of Crop Science, 5(4):421-427. (Journal)
Dewir, Y.H., El-Mahrouk, M.E. and Naidoo, Y. 2011. Effects of some mechanical and chemical treatments on seed germination of Sabal palmetto and Thrinax morrisii palms. Australian Journal of Crop Science, 5(3): 248-255. (Journal)
El-Dengawy, E.F.A. 2005. Promotion of seed germination and subsequent seedling growth of loaquat (Eriobotrya japonica) by moist-chilling and GA3 applications. Scientia Horticulturae, 105(3): 331-342. (Journal)
Elouaer, M.A. and Hannachi, C. 2012. Seed priming to improve germination and seedling growth of safflower (Carthamus tinctorius) under salt stress. EurAsian Journal of BioSciences, 6: 76-84. (Journal)
 
Finkelstein, R., Reeves, Ariizumi, T. and Steber, C. 2008. Molecular Aspects of Seed Dormancy.Annual Reviewof Plant Biology, 59: 387-415. (Journal)
Giba, Z., Grubisic, D., Todorovic, S., Stojakovic, D. and Konjevic, R. 1998. Effects of nitric oxide-releasing compounds on phytochrome-controlled germination of Empress tree seeds. Plant Growth Regulation, 26: 175-181. (Journal)
Hassani, S.B., Saboora, A., Rajabian, T. and Fallah Husseni, H. 2009. Effects of temperature, GA3 and cytokinins on breaking seed dormancy of Ferula assa-foetida L. Iranian Journal of Science and Technology, 33: 75-85. (In Persian)(Journal)
Kamal, J. 2010. Impact of allelopathy of sunflower (Helianthus annuus L.) roots extract on physiology of wheat (Triticum aestivum L.). African Journal of Biotechnology, 10: 14465-14477. (Journal)
Kato-Noguchi, H. and Macias, F.A. 2008. Inhibition of germination and α-amylase induction by 6-methoxy- 2-benzoxazolinone in twelve plant species. Biological Plantaum, 52: 351-354. (Journal)
Keshtkar, H.R., Azarnivand, H. and Atashi, H. 2009. Effect of prechilling and GA3 on seed germination of Ferula assa-foetida and Prangos ferulacea. Seed Science and Technology, 37(2): 464-468. (Journal)
Kucera, B., Cohn, M.A. and Leubner-Metzger, G. 2005. Plant hormone interactions during seed dormancy release and germination. Seed Science Research, 15: 281-307. (Journal)
Kulkarni, M.G., Street, R.A. and Staden J.V. 2007. Germination and seedling growth requirements for propagation of Diosscorea dregeana (Kunth) Dur. and Schinz-A tuberous medicinal plant. South Africa Journal of  Botany, 33: 131-137. (Journal)
Miller, K., Tintelnot, S. and Leubner-Metzger, G. 2006. Endosperm limited brassicaceae seed germination: abscisic acid inhibits embryo- induced endosperm weakening of Lepidium sativum (cress) and endosperm rupture of cress and Arabidopsis thaliana. Plant Cell Physiology, 47:864–877. (Journal)
Miransari, M. and Smith, D.L. 2014. Plant hormones and seed germination. Environmental and Experimental Botany, 99: 110-121. (Journal)

Monjam, S., Zenli, E., Ghaderifar, F., Soltani, E. and Hosseni Chaleshtri, M. 2016. Study the genetic variation of rice seed vigour by multivariate statistical methods. Electronic Journal of Crop Production, 8: 121-142. (In Persian)(Journal)

Nabaee, M., Roshandel, P.and Mohammadkhani, A.R. 2013. Effect of chemical treatments, pre-moist chilling, hot and tap water on seed dormancy breaking in Arctium lappa. Journal of Plant researches, 26: 217- 225. (In Persian)(Journal)
Nemati, A., Sharifi, H., Gerdakaneh, M. and Sharifi, Z. 2016. The effect of pre-chilling and gibberellic acid on breaking seed dormancy of two medicinal plants species Silybum Mrianum and Citrulus Colocynthis. Iranian Journal of Seed Research, 3: 170-176. (In Persian)(Journal)
Park, D.L. 2017. Methods of seed dormancy mesurance and dormancy breaking workshop, ISTA's 30th Annual Congress. 20-23 september, Seoul, South Korea. (Conference)
Patil, J.G., Ahire M.L. and Nikam, T.D. 2012. Influence of plant growth regulators on in vitro seed germination and seedling development of Digitalis purpurea L. The Asian and Australian Journal of Plant Science and Biotechnology, 6(1): 12-18. (Journal)
Peng, J. and Harberd, N.P. 2002. The role of GA-mediated signalling in the control of seed germination. Current Opinion in Plant Biology, 5(5): 376-381. (Journal)
Scott, S.J., Jones, R.A. and Williams, W.A. 1984. Review of data analysis methods for seed germination. Crop Science, 24: 1192-1199. (Journal)
Sharifi, H., Khajeh-Hosseini, M. and Rashed-Mohassel, M.H. 2015. Study of seed dormancy in seven medicinal species from Apiaceae. Iranian Journal of Seed Research, 2: 25-36. (In Persian)(Journal)
 
Sharifi, H., Nemati, A.and Gerdakaneh, M. 2017. Breaking seed dormancy and improve germina tion of four medicinal species of apiaceae by gibberellic acid and prechilling treatments. Iranian Journal of Seed Science and Research, 4(3): 27-38. (In Persian)(Journal)
Sikder, S., Hasan, M.A. and Hossain, M.S. 2009. Germination characteristics and mobilization of seed reserves in maize varieties as influenced by temperature regimes. Journal of Agriculture Ruralal Development, 2: 51- 56. (Journal)
Sumlu, S., Atar, H.H. and Khawar, K.M. 2010. Breaking seed dormancy of water lily (Nymphaea alba L.) under in vitro conditions. Biotechnology and Biotechnology Equipment, 24(1): 1582- 1586. (Journal)

Zargari, A. 1995. Pharmaceutical plants. Tehran Univcity Publication. (In Persian)(Book)
Iranian Journal of Seed Sciences and Research
Volume 8, Issue 2
July 2021
Pages 161-175

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