Improvement and regeneration of canola seeds (Brassica napus) with growth promoting compounds under different irrigation intervals

Document Type : Research Paper

Authors

1 Department of Soil Science, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran

2 Soil and Water Research Department, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Shiraz, Iran

Abstract

The use of growth promoting compounds is one of the possible ways to improve and restore seed quality. This study was conducted as a split plot experiment in a randomized complete block design with three replications in 2019. The main plots consisted of three irrigation intervals (irrigation at 40, 60 and 80% of available moisture depletion) and the subplots was different growth promoting compounds in six levels consisted of control, soil application of humic acid and foliar spray of amino acid, fulvic acid, seaweed extract and the combination of growth stimulants. Results showed that irrigation regime of 80% available moisture depletion decreased germination rate and seed vigor by 11.0% and 28.01% compared to 40% depletion respectively. Increase of irrigation intervals also increased seed electrical conductivity. At the irrigation level of 80% moisture depletion, maximum germination with the means of 87% and 85% was observed in the combination of growth stimulants and spray of amino acids respectively. Growth stimulants improved seedling dry weight. Under irrigation conditions of 60 and 80% moisture depletion, the combination of growth stimulants increased the alpha-amylase activity by 31.03 and 71.42% respectively. On the whole, considering the positive effects of growth promoting compounds on improving and regenerating the seeds of canola they can be recommended in drought stress conditions.

Keywords

References

Abdul Baki, A.A. and Anderson, J.D. 1973. Vigor determination in soybean seed by multiple criteria. Journal of Crop Science, 13: 630-633. (Journal)
Anjum, N.A., Gill, S.S. and Gill, R. 2014. Plant Adaptation to Environmental Change: Significance of Amino Acids and Their Derivatives (CABI). (Book)
Atarod, H., Irannejad, H., Shirani Rad, A.H., Amiri, R. and Akbari, Gh. 2012. Assessment of drought stress and planting date effects applied on original plant, on its seed electrical conductivity rate. Iranian Journal of Field Crops Research, 9(2): 242-247. (In Persian)(Journal)
Attarzadeh, M., Balouchi, H., Movahhedi Dehnavi, M., Salehi, A. and Rajaie, M. 2019a. Response of germination and electrical conductivity of seeds produced by Echinacea purpurea's mother plants under the influence of biological fertilizers and drought stress. Iranian Journal of Seed Science and Technology, 8(1): 185-200.(In Persian)(Journal)
Attarzadeh, M., Balouchi, H., Rajaie, M., Movahhedi Dehnavi, M. and Salehi, A. 2019b. Growth and nutrient content of Echinacea purpurea as affected by the combination of phosphorus with arbuscular mycorrhizal fungus and Pseudomonas florescent bacterium under different irrigation regimes. Journal of Environmental Management, 231: 182-188. (Journal)
Azadbakht, F., Amini Dehaghi, M. and Ahmadi, K.H. 2018. Effect of humic acid and folic acid on seed germination properties of echinacea purpurea under salt stress conditions. Journal of Seed Research, 8(3): 29-42.(In Persian)(Journal)
Balouchi, H.R., Bagheri, F., Kayednezami, R., Movahedi Dehnavi, M. and Yadavi, A.R. 2014. Effect of seed aging on germination and seedling growth indices in three cultivars of brassica napus. Journal of Plant Research, 26(4): 397-411. (In Persian)(Journal)
Battacharyya, D., Babgohari, M.Z., Rathor, P. and Prithiviraj, B. 2015. Seaweed extracts as biostimulants in horticulture. Scientia Horticulturae, 196: 39-48. (Journal)
Elouaer, M.A. and Hannachi, C. 2012. Seed priming to improve germination and seedling growth of safflower (Carthamus tinctorius) under salt stress. EurAsian Journal of BioSciences, 6: 76-84. (Journal)
FAO, 1979. Yield response to water. Irrigation and Drainage Paper No. 33, Rome. (Handbook)
Farooq, M., Wahid, A., Kobayashi, N., Fujita, D. and Basra, SMA. 2009. Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development, 29: 185-212. (Journal)
Flakelar, C.L., Luckett, D.J., Howitt, J.A., Dorana, G. and Prenzler, P.D. 2015. Canola (Brassica napus) oil from Australian cultivars shows promising levels of tocopherols and carotenoids, along with good oxidative stability. Journal of Food Composition and Analysis, 42: 179-186. (Journal)
Ghassemi-Golezani, K. and Ghassemi, S. 2013. Effects of water supply on seed development and quality of chickpea cultivars. Plant Breeding and Seed Science, 67: 37-44. (Journal)
Hadi, H., Daneshian, J., Hamidi, A., Asgharzade, A. and Zarghami, R. 2010. Effect of rhizobacteria on seedling characteristics of seeds produced under deficit irrigation. Agronomy Journal (Pajouhesh and Sazandegi), 86: 42-50. (In Persian)(Journal)
Halpern, M., Bar-Tal, A., Ofek, M., Minz, D., Muller, T. and Yermiyahu, U. 2015. The use of biostimulants for enhancing nutrient uptake. In: Sparks, D.L. (Ed.), Advances in Agronomy, 129: 141-174. (Journal)
Hamidi, A., Daneshian, J. and Asgharzadeh, A. 2016. A review of drought stress on mother plant effect on soybean seed germination and vigour improvement by some beneficial soil microorganisms treatment assessment. Iranian Journal of Seed Sciences and Research, 3(2): 109-124. (In Persian)(Journal)
ISTA, 2003. Handbook for seedling evaluation (3rd.ed). International Seed Testing Association Zurich, Switzerland, 223 pp. (Handbook)
Jardin, P. 2015. Plant biostimulants: definition, concept, main categories and regulation. Scientia Horticulturae, 196: 3-14. (Journal)
Kafi, M. and Mahdavi-Damghani, A. 2000. Mechanism of Tolerance to Environmental Stress in Sowings Plant. Ferdowsi University of Mashhad, 467 pp. (In Persian)(Book)
Khan, A., Guramni, A.R., Khan, M.Z., Hussain, F., Akhtar, M.E. and Khan, S. 2012. Effect of humic acid on growth, yield, nutrient composition, photosynthetic pigment and total sugar contents of peas (Pisum sativum L). Journal of Chemical Society of Pakistan, 6: 56-63. (Journal)
Kocira, A., Kornas, R. and Kocira, S. 2013. Effect assessment of Kelpak sl on the Bean yield. Journal of Central European Agriculture, 14(2): 67-76. (Journal)
Masondo, N.A., Kulkarni, M.G., Finnie, J.F. and Staden, J.V. 2018. Influence of biostimulants-seed-priming on Ceratotheca triloba germination and seedling growth under low temperatures, low osmotic potential and salinity stress. Ecotoxicology and Environmental Safety, 147: 43-48. (Journal)
Meskini Vishkaei, F., Mohammadi, M.H., Neishabouri, M.R. and Shekari, F. 2017. A model to estimate soil water depletion coefficient using plant and soil properties. Iranian Journal of Soil and Water Research, 48: 749-758. (In Persian)(Journal)
Morales-Cedillo, F., Gonzalez-Solis, A., Gutiérrez-Angoa, L., Cano-Ramírez, D.L. and Gavilanes-Ruiz, M. 2015. Plant lipid environment and membrane enzymes: the case of the plasma membrane H+-ATPase. Plant Cell Reports, 34: 617-629. (Journal)
Papenfus, H.B., Kulkarni, M.G., Posta, M., Finnie, J.F. and Staden, J.V. 2015. Smoke-isolated trimethylbutenolide inhibits seed germination of different weed species by reducing amylase activity. Weed Science, 63: 312-20. (Journal)
Rose, M.T., Patti, A.F., Little, K.R., Brown, A.L., Jackson, W.R. and Cavagnaro, T.R. 2014. A meta-analysis and review of plant-growth response to humic substances: practical implications for agriculture. In: Sparks, D.S. (Ed.), Advances in Agronomy, 124: 37–89. (Journal)
Soltani, A., Galashi, S., Zeinali, E. and Latifi, N. 2001. Germination, seed reserve utilization and seedling growth of chickpea as affected by salinity and seed size. Seed Science, 30: 51-60. (Journal)
Taimur, A., Khan, R. and Khattak, T.N. 2018. Effect of humic acid and fulvic acid based liquid and foliar fertilizers on the yield of wheat crop. Journal of Plant Nutrition, 1-8. (Journal)
Xiao, Z., Storms, R. and Tsang, A. 2006. A quantitative starch-iodine method for measuring alpha amylase and glucoamylase activities. Analytical Biochemistry, 351: 146-148. (Journal)
Zada, M., Zakir, N. Rabbani, M.A. and Shinwari, Z.K. 2013. Assessment of genetic variation in Ethiopian mustard (Brassica Carinata A. Braun) germplasm using multivariate techniques. Pakestanian Journal Botony, 45: 583-593. (Journal)
Iranian Journal of Seed Sciences and Research
Volume 7, Issue 4
January 2021
Pages 463-475

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