Investigating effect of salicylic acid on germination and activity of some enzymes antioxidant of Nigella sativa L. under salt stress conditions

Document Type : Research Paper


1 Ph.D of Genetic Engineering, Department of Agronomy and Plant Breeding, College of Agriculture, Ilam University, Ilam, Iran

2 Assistant Professor, Department of Agronomy and Plant Breeding, College of Agriculture, Ilam University, Ilam, Iran

3 Ph.D Candidate of Genetic Engineering, Department of Agronomy and Plant Breeding, College of Agriculture, Ilam University, Ilam, Iran


 In order to investigate the effect of salicylic acid on germination properties under salinity conditions, a factorial design based on complementary randomized design with three replications. The test factors consisted of salicylic acid at three levels (zero, 0.75 and 1.5 millimolar) and salinity stress at five levels (0, 100, 200, 300 and 400 millimolar). The effects of salicylic acid and salinity stress on seed vigor index, fresh weight and seedling dry weight were significant. The effects of salicylic acid and salinity on other traits were significant at 1% probability level. The results showed that salinity significantly reduced germination and seedling growth, but salicylic acid improved germination indices and increased germination in stressed treatments. With increasing salinity stress, the rate and germination percentage decreased. The maximum rate of germination was obtained in saline silicic acid (1.5mM) treatment under non salinity conditions. Pre-treatment significantly increased the activity of catalase enzyme compared to pretreatment and decreased malondialdehyde in salicylic acid treatments. Pre-treatment at a concentration of 1.5 (mM), while also having more effect on germination, increased the resistance of seedlings of Nigella sativa under salinity stress.


Alebrahim, M.T., Sabaghnia, N., Ebadi, A. and Mohebodini, M. 2004. Investigation the effect of salt and drought stress on seed germination of thyme medicinal plant (Thymus vulgaris). Journal Research in Agriculture Science, 1: 13-20. (Journal)
Ashraf, M. and Waheed, A. 1990. Screening of local exotic of lentil (Lens culinaris Medik) for salt tolerance at two growth stage. Plant and Soil, 128: 167-176. (Journal)
Basra, S.M.A., Afzal, I., Rashid, R.A. and Hameed, A. 2005. Inducing salt tolerance in soybean by seed vigor enhancement techniques. Journal of Biotechnology and Biochemical, 1: 173-179. (Journal)
Bray, C.M., Davision, P.A., Ashraf, M. and Taylor, R.M. 1989. Biochemical changes during osmopriming of leek seed. Annals of Botany, 63: 185-193. (Journal)
Burnett, S., Thomas, P. and Van Iersel, M. 2005. Postgermination drenches with PEG-8000 reduce growth of salvia and marigolds. HortScience, 40: 675-679. (Journal)
Burnett, S.E., Pennisi, S.V., Thomas, P.A. and Iersel, M.W. 2005. Controlled drought affects morphology and anatomy of Salvia solendens. Journal of the American SocietyforHorticulturalScience, 130(5): 775-781. (Journal)
Davey, M.W.E., Stals, B., Panis, J., Keulemans, R. and Swennen, L. 2005. High throughput determination of malondialdehyde in plant tissues. Analytical Biochemistry, 345: 201-207. (Journal)
Demiral, T. and Turkan, I. 2005. Comparative lipid peroxidation, antioxidant defens systems and proline content in roots of two rice cultivars differing in salt tolerance. Environmental and Experimental Botany, 53:247-257. (Journal)
El-Tayeb, M.A. 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation, 45: 215-225. (Journal)
Farhoudi. R., Saeedipour, S. and Mohammadreza, D. 2011. The effect of NaCl seed priming on salt tolerance, antioxidant enzyme activity, and prolin and carbohydrate accumulation of Muskmelon (Cucumis melo L.) under saline condition. African Journal of Agriculture Research, 6:1363-1370. (Journal)
Fathi Amirkhiz, K., Omidi, H., Heshmati, S. and Jafarzadeh, L. 2012. Accelerating reviews on seed vigor and germination characteristics in medicinal plant Black cumin (Nigella sativa L.) undr salinity stress. Iranian Journal of Fisheries Sciences, 10(2): 299-310. (In Persian)(Journal)
Francois, L.E. 1994. Growth, seed yield and oil content of canola grown under saline conditions. Agronomy Journal, 86: 233-234. (Journal)
Garratt, A., Schmidt, L., Mackintosh, A. and Fitzpatrick, R. 2002. Quality of life measurement: bibliographic study of patient assessed health outcome measures. Diabetic Medicine, 19(1):1-11. (Journal)
Gautam, S. and Singh, P.K. 2009. Salicylic acid induced salinity tolerance in corn grown under NaCl stress. Acta physiologiae Plantarum, 31: 1185-1190. (Journal)
Ghasemi Jobshahr, E. and Khoramivafa, M. 2012. Effect of pretreatment of salicylic acid on germination and seedling properties Callendulla officinalis in salt stress condition. Plant Production Technology, 12(2): 57-70. (In Persian)(Journal)
Hanan, E.D. 2007. Influence of salicylic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare. Biological Research, 1: 40-48. (Journal)
Heath, R.L. and Packer, L. 1969. Photoperoxidation in isolated chloroplastkinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 189-198. (Journal)
Hosseini, H. and Rezvani Moghadam, P. 2006. Effect of water and salinity stress in seed germination on Isabgol (Plantago ovata). Iranian Journal of Field Crops Research, 4(1):15-22. (In Persian)(Journal)
Ilan, B., Wentao, X., Einat, B., Mwafaq, I., Amnon, S., Aaron, F. and Efraim, L. 2012.  Distribution of primary and specialized metabolits in Nigella Sativa seeds, a spice with vast  traditional and historical uses. Molecules, 17: 10159-10177. (Journal)
Joudi, M. and Sharifzadeh, F. 2007. Study of the effect of hydropaymine on different barley varieties. Desert, 11(1):99-109. (In Persian)(Journal)
Kafi, M. and Rahimi, Z. 2010. The effect of different levels of salinity on plant germination properties of purslane (Portulaca oleracea L.). Iranian Journal of Agricultural Research, 8(4): 615-621. (In Persian)(Journal)
Kafi, M., Nezami, A., Hosseini, H. and Masoumi, A. 2006. Physiological effects of drought stress caused by polyethylene glycol on germination of lentil genotypes. Iranian Journal of Field Crops Research, 3(1):69-79. (In Persian)(Journal)
Katergi, N., Van Hoorn, J.W., Hamdy, A., Karam, F. and Mastrortilli, M. 1994. Effect of salinity on emergence and on water stress early seedling growth of sunflower and maize. Agricultural Water Management, 26: 81-91. (Journal)
Khorramdel, S., Koocheki, A., Nassiri Mahallati, M. and Ghorbani, R. 2011. Effect of biofertilizers on the yield and components of black cumin (Nigella sativa L.). Iranian Journal of Field Crops Research, 8(5):768-776.(In Persian)(Journal)
Krishramurthy, L., Ito, O., Johansen, C. and Saxsena, N.P. 1998. Length to weight ratio of chickpea roots under progressively reducing soil moisture conditions in a vertisol. Field Crops Research, 58: 177-185. (Journal)
Mazaheri Tirani, M. and Manouchehri Kalantari, K.H. 2007. Investigation of three factors of salicylic acid, drought stress and ethylene and their interaction on seed germination of Brassica napus L. Iranian Journal of Biology, 9:408-418. (InPersian)(Journal)
McCord, J.M. 2000. The evolution of free radicals and oxidative stress. American Journal of Medicine, 108:652 –659. (Journal)
Miller, T.R. and Chapman, S.R. 1978. Germination responses of three forage grasses to different concentration of six salts. Journal of Range Management, 31(2): 123-124. (Journal)
Mishra, A. and Choudhuri, M.A. 1999. Effects of salicylic acid on heavy metal induced membrane deterioration mediated by lipoxygenase in rice. Biologia Plantarum, 42: 409-415. (Journal)
Moosavi, A., Tavakkol-Afshari, R., Sharif-Zadeh, F. and Aynehband, A. 2009. Effect of seed priming on germination characteristics, polyphenol oxidase, and peroxidase activities of four amaranth cultivars. Journal of Food Agriculture Environment, 7: 353-358. (In Persian)(Journal)
Moradi, R. and Rezvani Moghaddam, P. 2010. Study the effects of prepriming seed with salicylic acid in salinity stress condition, on germination and growth characteristics of Foeniculum vulgareMill (Fennel). Iranian Journal of Crop Sciences, 8(3): 489-500. (In Persian)(Journal)
Orcutt, D.M. and Nilsen, E.T. 2000. The Physiology of Plants under Stress Soil and Biotic Factors. John Wiley and Sons Inc., New York, 680 p. (Book)
Panda, S.K. and Upadhyay, R.K. 2004. Salt stress induces oxidative alterations and antioxidative defense in in the roots of Lemna minor. Biologia Plantarum , 48(2): 249-253. (Journal)
Rajasekaran, L.R. and Blake, T. 1999. New plant growth regulator protect photosynthesis and enhance growth under drought of jack pine seedlings. JournalofPlant Growth Regulation, 18:175-181.(Journal)
Ramana, S., Biswas, A.K., Kundu, S., Saha, J.K. and Yadava, R.B.R. 2002. Effect of distillery effluent on seed germination in some vegetable crops. Bioresource Technology, 82 (3): 273-275. (Journal)
Redmann, R.E. and Belyk, M. 1994. Growth of transgenetic and standard canola (Brassica napus L.) varieties in response to soil salinity. Canadian Journal of Plant Science, 74(4): 797-799. (Journal)
Safarnejad, A. and Hamidi, H. 2008. Study of morphological characters of Foeniculum vulgare under salt stress. Iranian Journal of Rangeland and Forests Plant Breeding and Genetic Research, 16(1):125-140. (In Persian)(Journal)
Senaranta, T., Touchell, D., BumM, E. and Dixon, K. 2002. Acetylsalicylic (aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulation, 30: 157-161. (Journal)
Shakirova, F.M. and Bezrukova, M.V. 1997. Induction of wheat resistance against environmental salinization by salicylic acid. Biology Bulletin, 24: 109–112.
Sharikova, F., Sakhabutdinova, A., Bezrukova, M., Fatkhutdinova, R. and Fatkhudinova, D. 2003. Changes in the hormonal status of wheat seedling induced by salicylic acid and salinity. Plant Science, 164:317-322. (Journal)
Velikova, V., Yordanov, I. and Edreva, A. 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Plant Science, 151: 59-66. (Journal)
Wang, L., Chen, S., Kong, W., Archbold, D.D. and Li, S. 2006. Salicylic acid pretreatment alleviates chilling injury and affects the antioxidant system and heat shock proteins of peaches during cold storage. Postharvest Biology and Technology, 41: 244-251. (Journal)
Zehtabsalmasi, S. 2008. The effect of salinity on seed germination and pre-treatment of German chamomile. Research and Agriculture, 2(2): 28-30. (In Persian)(Journal)