The effects of seed priming on some germination index, enzymatic activity and some biochemical traits of Capparis spinosa and Kelussia odoratissima

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Agronomy and Plant Breeding, Azarbaijan Shahid Madani University, Tabriz, Iran

2 Assistant Professor, Department of Agricultural Biotechnology, Azarbaijan Shahid Madani University, Tabriz, Iran

Abstract

In order to study the effects of seed priming (GA3, KNO3, temperature and soaking) on seed germination and some physiological characteristics (protein content and prolin cantent) and enzymitc activity (catalase, peroxidase, α-amilaze ) of Capparis spinosa and Kelussia odoratissima, two Separate experiment were conducted as completely randomized design. Treatments used in this experiment had significant effects on seed germination percentage, shoot and root length, protein content and α-amylase enzyme content in both plants. The results showed that 1 and 2 mg L-1 GA3 increased root length and 2 mg L-1 GA3 increased shoot length in both plants. 1 and 2 mg L-1 KNO3 had positive effects on protein content. one and 2 mg L-1 KNO3, soaking and keeping at 40c for 30 and 60 days increased protein content in Capparis spinosa. The highest amount of α-amilaze enzyme in Capparis spinosa was obtained from keeping seeds at 60c for 60 days and in Kelussia odoratissima, the highest data was recorded at maintenance at 40c for 90 days and soaking and keeping at 40c for 30, 60 and 90 days. The top quantities of seed germination percentage were obtained from soaking, then maintenance at 40c for 30 days in Kelussia odoratissima and for 30 and 60 days in C. spinosa. In Kelussia odoratissima, the highest data for Catalase enzyme was recorded at 40c and 60c after 90 days. The highest data for Proline content was obtained from 2 mg L-1 GA3 and soaking and keeping at 40cfor 90 days. Finally, our results showed that seed priming with GA3 and soaking, then maintenance at 40c for 30 and 60 days had positive effects on seed germinations in both studied plants.

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Iranian Journal of Seed Sciences and Research
Volume 7, Issue 2
July 2020
Pages 229-240

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