Effect of aging of seed and hydro-priming on germination characteristics and activity of some antioxidant enzymes of hybrid corn (Zea mays L.)

Document Type : Research Paper

Author

Ph.D. of Crop Physiology, Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran

Abstract

In order to investigate the effects of accelerated aging and hydro-priming of seed on germination and growth characteristics of corn seedling (cv. single cross 701), a factorial experiment was conducted based on completely randomized design with three replications in the research laboratory of University of Maragheh, Iran in 2015 year. Factors included three level of accelerated aging of seed (zero (control) and 2 and 4 days) and three level of pre-treatment of seed (Non-priming and hydro-priming for a period of 12 and 24 hours). The results of analysis of variance (ANOVA) showed that the simple effect of aging of seed and hydro-priming on all studied traits was significant at 1% probability level, but only the interaction of aging of seed and hydro-priming was significant on ascorbate peroxidase activity, electrolyte leakage rate, germination percent and germination rate. Simple and interaction effects of aging of seed and hydro-priming on the ratio of plumule to radicle were non-significant. The results of the mean comparison showed that all of the traits were reduced due to aging of seed. The soluble proteins contents, superoxide dismutase activity, plumule length, radicle length and seed vigor index due to seed aging of 2 days were 13.7, 10.9, 14.8, 14.8 and 33.2%, respectively, and due to seed aging of 4 days, they decreased by 36.8, 33.4, 32.4, 35.0 and 67.9%, respectively, relative to the control (without aging of seed). The results indicated that by increasing the duration of the hydro-priming of seeds from 12 to 24 hours, the soluble proteins contents, superoxide dismutase activity, plumule length, radicle length and seed vigor index were increased. Based on the results of interaction effects it was found that in the non-aged seed (control) applying hydro-priming for 24 hours was the highest and in the aged seeds (for 4 days), no hydro-priming had the least ascorbate peroxidase activity and percentage and germination rate. According to the results of this research, it can be stated that hydro-priming (especially for 24 hours) can decrease some of the destructive and minor effects of seed aging, and this technique can be used for reduction of the damage caused by the aging of corn seeds.

Keywords

References

Abdoli, M. 2014. Effect of seed priming on seed dormancy, vigor and seedling characteristics of fennel (Foeniculum vulgare L.). Acta Advances in Agricultural Sciences, 2(8): 18-24. (Journal)
Abdoli, M. and Rasaei, B. 2018. The effects of hydro-priming and osmo-priming using nitrate salts on germination indices of soybean seedling (Glycine max L.). Journal of Seed Research, 7(4): 11-22. (In Persian)(Journal)
Abdul-Baki, A.A. and AndersonJ.D. 1973. Vigor determination in soybean by multiple criteria. Crop Science, 13: 630-633. (Journal)
Afzal, I., Basra, S.M.A., Ahmad, N. and Farooq, M. 2005. Optimization of hormonal priming techniques for alleviation of salinity stress in wheat (Triticum aestivum L.). Caderno de Pesquisa série Biologia, 17(1): 95-109. (Journal)
Agrawal, R.L. 1991. Seed Technology. Oxford and IBH, Publishing. 258 p. (Book)
Ahmadi, M. and Masoudi, B. 2017. Investigation of accelerated aging on germination and growing of sesame (Sesamum indicum L.) seedlings varieties. Code: ICANC-2/211, 2nd National Congress of Healthy Agriculture, Nutrition, Society. Tehran. 1-10 p. (In Persian)(Conference)
Ansari, O. and Sharif Zadeh, F. 2012. Slow moisture content reduction (SMCR) can improve some seed germination indexes in primed seeds of Mountain Rye (Secale montanum) under accelerated aging conditions. Journal of Seed Research, 2(2): 68-76. (In Persian)(Journal)
Bailly, C., Benamar, A., Corbineau, F. and Côme, D. 2000. Antioxidant systems in sunflower (Helianthus annuus L.) seeds as affected by priming. Seed Science Research, 10(1): 35-42. (Journal)
Basra, S.M.A., Ahmad, N., Khan, M.M., Iqbal, N. and Cheema, M.A. 2003. Assessment of cotton seed deterioration during accelerated ageing. Seed Science and Technology, 31(3): 531-540. (Journal)
Bayat, M. and Rabiee, B. 2006. The effect of cold stress and aging on the components germination and seedling growth of five Rapeseed. Journal of Agricultural Science and Natural Resources, 2(7): 46-57. (In Persian)(Journal)
Beauchamp, C. and Fridovich, I. 1971. Superoxide dismutases: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44(1): 276-287. (Journal)
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2): 248-254. (Journal)
Copeland, L.O. and Mcdonald, M.B. 1985. The Chemistry of seeds. In Principles of seed science and technology, 2nd Ed., Macmillan Publ. Company, Macmillan. INS, New York. (Book)
Darabi, F., Valipour, M., Naseri, R. and Moradi, M. 2017. The effects of accelerated aging test on germination and activity of antioxidant enzymes of maize (Zea mays) hybrid varieties seeds. Iranian Journal of Seed Research, 4(1): 45-59. (In Persian)(Journal)
De Figueiredo, E., Albuquerque, M.C. and De Carvalho, N.M. 2003. Effect of the type of environmental stress on the emergence of sunflower (Hellianthus annns L.), soybean (Glycine max L.) and maize (Zea mays L.) seeds with different levels of vigor. Seed Science and Technology, 31: 465-479. (Journal)
 
Demir Kaya, M., Dietz, K.J. and Sivritepe, H.O. 2010. Changes in antioxidant enzymes during ageing of onion seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(1): 49-52. (Journal)
Demir Kaya, M., Okçu, G., Atak, M., Çikili, Y. and Kolsarici, Ö. 2006. Seed treatment to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24(4): 291-295. (Journal)
Ghanbari, M., Modarres-Sanavy, S.A.M., Mokhtassi Bidgoli, A. and Talebi-Siah Saran, P. 2018. Effect of hydropriming and seed aging on seed germination and biochemical characteristics of pinto bean (Phaseolus vulgaris) seed under salt stress. Iranian Journal of Seed Research, 4(2): 37-55. (In Persian)(Journal)
Goodarzian, M., Ghasemi, E., Mansouri-Far, C., Saeidi, M. and Heidari, Z. 2014. The effect of accelerated aging on germination indices and activity of antioxidant enzymes in different hybrids of maize embryos. Journal of Seed Research, 4(2): 38-44. (In Persian)(Journal)
ISTA. 2003. Handbook for Seedling Evaluation. 3rd edition, International Seed Testing Association, Zurich, Switzerland. 223 p. (Handbook)
Jahanbakhshi, A., Ebdali, A., Sharafizadeh, M. and Habibi Khaneyani, B. 2012. Osmo-priming effects on germination and yield of the vetch under exhaustion. Journal of Crop Physiology, 4(16): 19-32. (In Persian)(Journal)
Janmohammadi, M., Fallahnezhad, Y., Golshan, M. and Mohammadi, H. 2008. Controlled ageing for storability assessment and predicting seedling early growth of canola cultivars (Brassica napus L.). Journal of Agricultural and Biological Science, 3(5-6): 22-26. (Journal)
Kaur, S., Gupta, A.K. and Kaur, N. 2002. Effect of osmo- and hydropriming of chickpea seeds on seedling growth and carbohydrate metabolism under water deficit stress. Plant Growth Regulation, 37(1): 17- 22. (Journal)
Kaur, S., Gupta, A.K. and Kaur, N. 2005. Seed priming increases crop yield possibly by modulating enzymes of sucrose metabolism in chickpea. Journal of Agronomy and Crop Science, 191: 81-87. (Journal)
Khan, M., Qasim, M., Javid Iqbal, M., Naeem, A. and Abbas, M. 2003. Effect of seed humidification on germinability, vigor and leakage in Cockscom (Celosia argentea L.). International Journal of Agriculture and Biology, 5: 499-503. (Journal)
Khoshkharam, M., Shahrajabeyan, M. Soleimani, A. and Fathi, G. 2010. Burnout effect on seed germination and seedling growth components two safflower varieties. 5th National Conference of New Ideas in Agriculture. Khorasgan-Islamic Azad University, Khorasgan Branch. Iran. pp: 1-3. (In Persian)(Conference)
Kibinza, S., Vinel, D., Côme, D., Bailly, C. and Corbineau, F. 2006. Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiologia Plantarum, 128(3): 496-506. (Journal)
Maguire, J.D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2: 176-177. (Journal)
Mansouri-Far, C., Goodarzian-Ghahfarokhi, M., Saeidi, M. and Abdoli, M. 2015. Antioxidant enzyme activity and germination characteristics of different maize hybrid seeds during ageing. Environmental and Experimental Biology, 13: 177-182. (Journal)
McDonald, M.B. 1999. Seed determination: physiology, repair and assessment. Seed Science and Technology, 27: 177-237. (Journal)
McDonough, C.M., Floyd, C.D., Waniska, R.D. and Rooney, L.W. 2004. Effect of accelerated aging on maize, sorghum, and sorghum meal. Journal of Cereal Science, 39(3): 351- 361. (Journal)
Mohajeri, F., Ramroudi, M., Taghvaei, M. and Galavi, M. 2017. The effect of accelerated aging on seed germination traits produced from treated mother plant in three bean cultivars. Iranian Journal of Seed Science and Technology, 6(1): 101-112. (In Persian)(Journal)
Mohamadzadeh, A., Majnonhoseini, N., Ghafari, M., Asadi, S., Dosti, A. and Khavazi, K. 2012. Effect of aging on germination of seeds and bacteria growth and yield of two cultivars of red Lvyba (Pharsalus vulgaris L). Iranian Journal of Crop Sciences, 43(4): 589-600. (In Persian)(Journal)
Mohammadi, H., Soltani, A., Sadeghipour, H.R. and Zeinali, E. 2011. Effect of seed aging on subsequent seed reserve utilization and seedling growth in soybean. International Journal of Plant Production, 5(1): 65-70. (Journal)
 
Nahofte Esterabad, A., Rahemi Karizaki, A. and Nakhzari Moghadam, A. 2016. Effect of seed deterioration on germination parameters and growth seedling of two maize varieties. Iranian Journal of Seed Science and Research, 3(2): 1-11. (In Persian)(Journal)
Najafi, G., Khomari, S. and Javadi, A. 2016. The response of rapeseed seed germination to seed vigor and hydro-priming changes. Journal of Seed Research, 5(4): 54-70. (In Persian)(Journal)
Nakano, Y. and Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22(5): 867-880. (Journal)
Pukacka, S. and Ratajczak, E. 2005. Production and scavenging of reactive oxygen species in Fagussylvatica seeds during storage at varied temperature and humidity. Journal of Plant Physiology, 162: 873-885. (Journal)
Ranjan, R., Bohra, S.P. and Jeet, A.M. 2001. Book of Plant Senescence. Jodhpur, Agrobios New York. pp. 18-42. (Book)
Sadrabadi Haghighi, R. 2014. Effects of accelerated aging and acid scarification of seed coat with hydrochloric acid on seed germination characteristics of chickpea c.v. Kaka. Journal of Crop Ecophysiology, 7(4): 485-498. (In Persian)(Journal)
Seiadat, S.A., Moosavi, A. and Sharafizadeh, M. 2012. Effect of seed priming on antioxidant activity and germination characteristics of Maize seeds under different aging treatments. Research Journals of Seed Science, 5(2):51-62. (Journal)
Sheidaei, S., Heidari Sharisabad, H., Hamidi, A., Noormohammadi, G. and Moghaddam, A. 2018. Impact of storage period of soybean seed on lipids peroxidation, soluble sugars, protein, electrical conductivity, quality characteristics and seedling emergence. Iranian Journal of Seed Science and Research, 5(4): 46-60. (In Persian)(Journal)
Siviritepe, H.O. and Dourado, A.M. 1995. The effects of priming treatments on the viability and accumulation of chromosomal damage in aged pea seeds. Annual Botany, 75: 165-171. (Journal)
Soltani, A., Kamkar, B. Ghaleshi, S. and Ghaderi, F. 2009. Deterioration effect on seed germination of wheat in response to environmental stresses. Electronic Journal of Crop Production, 2(2): 43-58. (In Persian)(Journal)
Tammela, P., Vaananen, P.S., Lakso, I., Hopia, A., Vourela, H. and Nygrem, M. 2005. Tocopherols, tocoterienols and fatty acids as indicators of natural ageing in Pinus syluestris seeds. Scandinavian Journal of Forest Research, 20(5): 378-384. (Journal)
Tavakol Afshari, R.S., Rashidi, S. and Alizadeh, H. 2009. Effects of seed aging on germination characteristics and on catalase and peroxidase activities in two canola cultivars (Brassica napus L.). Iranian Journal of Field Crop Science, 40: 125-133. (In Persian)(Journal)
Yao, Z., Liu, L., Gao, F. and Rampitschi, C. 2012. Development and seed aging mediated regulation of antioxidative genes and differential expression of proteins during pre and post-germinative phases in pea. Journal of Plant Physiology, 169: 1477-1488. (Journal)
Younesi, E., Bahari, A.A., Azadi, M.S. and Ansari, O. 2014. The effect of hydropriming and accelerated aging on germination indexes and catalase enzyme in pearl millet (Panicum miliaceum). Journal of Seed Research, 3(4): 61-70. (In Persian)(Journal)
Iranian Journal of Seed Sciences and Research
Volume 7, Issue 2
July 2020
Pages 147-159

Files

Share

How to cite

Statistics