Detection of Xanthomonas campestris pv. phaseoli and use of optimized method in bean seed lots health monitoring

Document Type : Research Paper

Authors

1 Research Assistant Professor, Seed and Plant Certification and Registration Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

2 Researcher, Seed and Plant Certification and Registration Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

3 Ph.D of Plant Diseases, Ferdowsi University of Mashhad, Khorasan Razavi, Mashhad, Iran

Abstract

Common bacterial blight, caused by the seed-borne bacteria Xanthomonas axonopodis pv. phaseol,  is important seed borne disease in been culture area in the world. Using healthy and certified seed is the major key to success for control disease distribution so seed health testing is routinely carried out in the certification programs. In this study cultivation diagnostic tests on selective medium, PCR and pathogenicity on susceptible cultivar Khomain for detection of the bacteria X. axonopodis pv. phaseoli were optimized. These optimized methods were used for assessing the health of bean seed samples obtained from different seed lots. The results showed that the use of selective-medium in culturing bacteria prior to PCR test, greatly reduces differentiation error of similar non-target colonies from pathogenic ones. Also performing PCR on suspected colonies isolated from selected media compared to PCR of seed extract, due to the removal of plant inhibitors, it reduces the possibility of false feedback. The mentioned methods check the health of selected seed samples from standard seed farms successfully and the results indicated the authentication seed health and non-contaminated seed lots.

Keywords

References

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Iranian Journal of Seed Sciences and Research
Volume 7, Issue 1
June 2020
Pages 69-77

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