Effect of organic acids and salt stress on germination of seed and physiological properties of (Echinacea Purpurea L.)

Document Type : Research Paper

Authors

1 M.Sc. Student of Seed Science and Technology, Faculty of Agricultural Sciences, University of Shahed, Tehran, Iran

2 Associate Professor, Faculty of Agricultural Sciences, University of Shahed, Tehran, Iran

3 M.Sc. Graduated of Agronomy, Faculty of Agricultural Sciences, University of Shahed, Tehran, Iran

Abstract

In order to investigate the effect of humic and fulvic acid on physiological properties of seedling Echinacea purpurea under salinity, a factorial experiment was conducted in a completely randomized design with three replications at Seed Technology Laboratory Shahed University in year 2016. The treatments experiment included salinity at four levels (0, 4, 8 and 12 ds/m), humic acid in three levels (0, 200 and 400 mg/lit) and fulvic acid at three levels (0, 200 and 400 mg/lit). The studied traits included germination percentage, relative water content, chlorophyll a, b and total content, carotenoid, proline and protein content. According to the results of analysis of variance, interaction effect of humic acid, fulvic acid and salinity had a significant effect on traits except the relative water content. The highest germination percentage was obtained by application of humic acid 200 mg/lit and fulvic acid 400 mg/lit with an average of 75.55% in salinity. According to the results of this study, humic acid has the most effect on the of soluble compounds content, as long as the fulvic acid concentration is 200 mg/lit or zero, it is possible to see the increase of soluble compounds at the levels of humic acid. The concentration of 400 mg/lit humic acid in the absence of fulvic acid and lack of salt stress had the highest chlorophyll total content. Salinity stress reduced seed germination of Sarkhargol, Application of Humic Acid and fulvic Acid Effects Caused by Salt Stress, though slightly improved.

Keywords

References

Amiri, B.M., Rezvani Moghaddam, P., Ehyai, M.R., Fallahi, J. and Aqhhvany Shajari, M. 2009. Effect of osmotic stress on germination indices and seedling growth of two medicinal plants Cynara scolymus and Echinacea purpurea. Environmental Stresses in Agricultural Sciences, 3(2): 165-176. (In Persian)(Journal)
Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Poly phenol oxidase in Beta vulgaris. Plant Physiology, 24: 1 -150. (Journal)
Azarnia, M. and Eisvand, H.R. 2013. Effects of hydro and hormonal priming on yield and yield components of chickpea in irrigated and rain-fed conditions. Journal of Crop Production, 6(4): 1-18. (In Persian)(Journal)
Bajji, M., Kinet., J.M. and Lutts, S. 2002. Osmotic and ionic effects of NaCl on germination, early seedling growth, and ion content of Saltbush (Atriplex halimus L.) (Chenopodiaceae). Canadian Journal of Botany, 80: 297-304. (Journal)
Bates, L.S., Waldern, R.P. and Tear, I.D. 1973. Rapid determination of free proline for water stress studies. Plant Soil, 39: 205-207. (Journal)
Blumenthal, M., Lindstrom, A., Lynch, M.E. and Rea, P. 2011. Herb sales continue growth up 3.3% In 2010. Herbal Gram, 90: 64-67. (Journal)
Ceeh, R. 2006. Phytochemical variation within populations of Echinacea purpurea (Asteraceae). Biochemical Systematics and Ecology, 30: 837-54. (Journal)
Celik, O. and Atak, C. 2012. The effect of salt stress on antioxidative enzymes and proline content of two Turkish tobacco varieties. Turkish Journal of Biology, 36(3): 339-356. (Journal)
Davoudi Fard, M., Habibi, D. and Davoudifard, F. 2012. Effect of salinity stress on cytoplasmic membrane stability, chlorophyll content and yield components in wheat, inoculated with Growth Stimulating Bacteria and humic acid. Journal of Agronomy and Plant Breeding, 8(2): 86-71. (In Persian)(Journal)
De Araujo, A.M., Silveira, A.G., Almeida, T.D., Rocha, M.A., Morais, D.L. and Viegas, R.A. 2006. Salinity tolerance of halophyte Atriplex nummularia L. grown under increasing NaCl levels. Engenharia Agricola e Ambiental, 10: 848- 854. (Journal)
Dehshiri, A., Modarres Sanavi, M., Rezaie, H. and Shirani Rad, A. 2012. Effect of elevated concentration of atomospheric carbon dioxide on some traits of three rapeseed (Brassica napus L.) varieties under saline conditions. Seed and Plant Production, 28(2): 35-52. (In Persian)(Journal)
Ebrahimi, M. and Miri, E. 2016. Effect of humic acid on seed germination and seedling growth of Borago officinalis and Cichorium intybus. Ecopersia, 4(1): 1239-1249. (In Persian)(Journal)
Eizadi, Z., Soroshzadeh, A., Modares Sanavi, S.A.M., Asnaashari, M., Aghaalikhani, M. and Davodi, P. 2014. Effect of extraction of aerial part extract of Echinacea Purpurea L. on its antimicrobial property in a number of pathogens. Journal of Rafsanjan University of Medical Sciences, 13: 267-280. (In Persian)(Journal)
El-Bassiony, A.M., Fawzy, Z.F., Abd El-Baky, M.M.H. and Asmaa, R.M. 2010. Response of snap bean plants to mineral fertilizers and humic acid application. Research Journal of Agriculture and Biological Sciences, 6(2): 169-175. (Journal)
Ghasemi­ Lemraski, M. 2013. The effect of seed priming on the germination of wheat (Triticum aestivum L.). Journal of Sciences and Seed Technology, 3(1): 1-9. (In Persian)(Journal)
Gholami, H. 2012. Effects of humic acid and folic acid on plant resistance to salinity stress. Senior Researcher of Agriculture, Faculty of Agriculture, Islamic Azad University, Garmsar Branch. (In Persian)(Handbook)
Gu, Z., Chen, D., Han, Y., Chen, Z. and Gu, F. 2008. Optimization of carotenoids extraction from Rhodobacter sphaeroides. Learning With Technologies, 41: 1082–1088. (Journal)
International Seed Testing Association (ISTA). 2010. International Rules for Seed Testing, Bassersdorf, Switzerland. (Handbook)
Jing-min, Z., Shang-jun, X., Mao-peng, S., Bing-yao, M., Xiu-mei, C. and Chunsheng, L. 2010. Effect of humic acid on poplar physiology and biochemistry properties and growth under different water level. Journal of Soil and Water Conservation, 8(1): 9-20. (Journal)
 
 
Kiamoghadam, M.R. and Bagheriya Najjar, M.B. 2009: Some physiological and biochemical parameters in Atrec Ql4A mutated plants under salinity stress. Journal of Plant Production Research, 16(1): 132-115. (In Persian)(Journal)
Manivannan, P., Jaleel, C.A., Sankar, B., Kishurekumar, A., Somasundaram, R., Lakshmanan, G.M. and Panneerselvam, R. 2007. Growth, biochemical modifications and proline metabolism in Helianthus annuus L. as induced by drought stress. Colloids and Surfaces B: Biointerfaces, 59: 141-149. (Journal)
Miri, Y. and Mirjalili, S.A. 2013. Effects of salinity stress on seed germination and some physiological traits in primary stages of growth in purple coneflower (Echinacea Purpurea). International Journal of Agronomy and Plant Production, 4(1): 142-146. (In Persian)(Journal)
Nazarbeygi, E., Lari Yazdi, H., Naseri, R. and Soleimani, R. 2011. The effects of different levels of salinity on proline and chlorophylls a, b in canola. American-Eurasian Journal of Agriculture and Environmental Science, 10: 70-74. (In Persian)(Journal)
Nouri, K., Omidi, H., Naghdi badi, H.A., Torabi, H. and Fotokian, M.H. 2013. Effects of soil and water salinity on flower yield, soluble compounds, content of saline elements and essential oil quality of German chamomile (Shirazian Babooneh, Matricaria recutita L.). Journal of Water Research in Agriculture, 26(4): 367-379. (In Persian)(Journal)
Ozturk, L., Demir, Y., Unlukara, A., Karatas, I., Kurunc, A. and Duzdemir, O. 2012. Effects of long-term salt stress on antioxidant system, chlorophyll and proline contents in pea leaves. Romanian Biotechnological Letters, 17(3): 7227-7236. (Journal)
Paravar, A., Omidi, H., Esanejad, S.N. and Amirzadeh, M. 2015. Seed hydroperimigation effect on germination and seedling growth of Sarkhrgol under salt stress. Journal of Seed Ecophysiology, 1(1): 57-69. (In Persian)(Journal)
Rahi, A., Davoudi Fard, M., Azizi, M. and Habibi, D. 2012. Investigating the effects of different amounts of humic acid and study of the process of response curves in Dactylis glomerata. Journal of Agronomy and Plant Breeding, 8(3): 28-15. (In Persian)(Journal)
Samavat, S. and Malakooti, M. 2005. Important use of organic acid (humic and fulvic) for increase quantity and quality agriculture productions. Journal of Water and Soil Science, 463: 1-13. (In Persian)(Journal)
Sarani, S., Heidari, M., Glavi, M. and Siahsar, B.A. 2013. Effects of salinity and iron on growth, photosynthetic pigments and electrophoresis bands in two genus chamomile (Matricaria chamomilla L. and Anthemis nobilis L.). Iranian Journal of Medicinal and Aromatic Plants Research, 29(4): 732-746. (In Persian)(Journal)
Sebahattin, A. and Necdet, C. 2005. Effects of different levels and application times of humic acid on root and leaf yield and yield components of forage Turnip (Brassica rapa L.). Agronomy Journal, 4: 130-133. (Journal)
Turkan, I. and Demiral, T. 2009. Recent developments in understanding salinity tolerance. Environmental and Experimental Botany, 67: 2-9. (Journal)
Turkman, O., Demir, S., Sensoy, S. and Dursun, A. 2005. Effect of Arbuscular Mycorrhizal fungus and Humic acid on the seedling development and nutrient content of pepper grown under saline soil conditions. Biological Sciences, 5: 565-574. (Journal)
Valentinuz, O.R., and Tollenaar, M. 2006. Effect of genotype, nitrogen, plant density, and row spacing on the area-per-leaf profil in maize. Agronomy Journal, 98: 94-99. (Journal)
Weisany, W., Sohrabi, Y., Heidari, G., Siosemardeh, A. and Ghassemi-Glezani, K. 2012. Changes in antioxidant enzymes activity and plant performance by salinity stress and zinc application in soybean Glycine max (L.). Plant Omics Journal, 5(2): 60-67. (Journal)
Iranian Journal of Seed Sciences and Research
Volume 7, Issue 1
June 2020
Pages 27-40

Files

Share

How to cite

Statistics