Effect of seed priming and aging on germination, biochemical traits and antioxidant enzyme gene expression in common bean (Phaseolus vulgaris l.)

Document Type : Research Paper


1 M.Sc. Student of Agronomy, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabili, Iran.

2 Professor, Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabili, Iran.

3 Associate Professor, Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabili, Iran.

4 Professor, Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabili, Iran

5 Ph.D. student, Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabili, Iran


In order to investigate the effect of priming and aging on seed germination and biochemical constituents of common bean seed a factorial experiment was conducted based on completely randomized design at the University of Mohaghegh Ardabili in 2016 with 3 replications. Treatments were aging (control without aging, 88% and 78% of germination of control) and priming (control, hydro-priming, priming by gibberellin and salicylic acid). After RNA extraction and cDNA synthesis, gene expression of antioxidant enzymes was studied using qRT-PCR and some of physiological and biochemical traits were measured. The results showed that the longest radicle and plumule, the highest radical and plumule fresh and dry weight and the highest phosphate reserves was related to gibberellin priming and non-aged seeds. Also, peroxidase gene expression was higher in salicylic acid pret-reatment with 88% aging level than the other antioxidant enzymes. With increasing the aging there was a reduction trend in the gene expression and the lowest expression observed for peroxidase in non-primed seeds in 78% of germination. In conclusion, priming with gibberellin caused to invigoration of weak bean seeds.


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