Effect of bulk and nano zinc oxide on seed germination and growth indices in tobacco (Nicotiana tabacum L.) seedlings

Document Type : Research Paper

Authors

1 Ph.D Student of Plant Physiology, College of Basic Sciences, University of Lorestan, Khorramabad, Iran

2 Assistant Professor of Plant Physiology, College of Basic Sciences, University of Lorestan, Khorramabad, Iran

3 Associate Professor, Department of Agronomy and Plant Breeding, College of Agriculture, University of Lorestan, Khorramabad, Iran

Abstract

The applications of bulk and nano zinc oxides have been increased recently, which can cause widespread plant's environment contaminations. This study investigated the effects of commercial bulk ZnO (˂1,000 nm) and nano ZnO (25 nm) on Nicotiana tabacum. Seeds were treated with ten concentrations of two forms (bulk and nano) ZnO (0.04, 0.2, 2.5, 5, 10, 50, 100, 500, 1000 and 2000 ppm) and dionized water used as control, at three replications. Number of germinated seeds was counted until 14th days. Some quantify and quality traits were investigated on 21th day. The results showed that both forms had no positive effect onfinal germination percentage (FGP) and time to reach 50% germination (T50%) but mean of germination time (MGT) and coefficient of uniformity of germination (CUG) were increased, especially by high concentrations of nano-form. Nano-ZnO (50 ppm and upper) and the bulk ZnO treatments (100 ppm and upper) decreased the length of hypocotyl, but inhibitory effects on radical length appeared at the lower levels of bulk form (0.2 ppm and upper) and all nano-ZnO levels. Most of treatments, especially at high concentrations, showed a negative effect on the tolerance indices such as relative hypocotyl length, root tolerance, germination tolerance, and allometric coefficient.These inhibitory effects were happened in the lower levels of nano-form compared to bulk-form. Pallid cotyledon’s color and asleep seedlings, which were observed at upper than 100 ppm, could be due to decreased chlorophyll and some changes in the amount of growth regulators.

Keywords


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