The effect of different treatments on seeds dormancy and germination of Gundelia tournefortii

Document Type : Research Paper


1 Assistant Professor, Faculty of Natural Resources, University of Kashan, Kashan, Iran

2 Ph.D student of Combat Desertification, Faculty of Natural Resources, University of Kashan, Kashan, Iran


Due to the increasing importance of on multiple uses native plants of dry lands, methods of improving seed germination rates of Gundelia tournefortii, a cold and arid land species with pharmacological and edible values, were investigated. Dormancy breaking treatments were applied in a completely randomized design. Seeds were then set in a germinator (25±1˚C - 70±3 %) and germination velocity and percentage and radicle and plumule length were recorded. Different treatments were applied including humic acid (for 26 and 54 mg/L), scarification H2SO4 (10 and 20 min), soaking in 300 and 500 ppm gibberellic acid (for 24 and 48 hours), temperature (15 and 30 min- 70˚C), Chilling (7 and 14 days- 4˚C), a complex treatment of Chilling (7 and 14 days- 4˚C) and gibberellic acid 500 ppm for (24 and 48 hours), and distilled water as control treatment. Results showed germination percentage and velocity and radicle and plumule length increase in all treatments rather than control. Only sulfuric acid and heat treatments prevented adequate radicle and plumule length growth. In the current study, the most successful treatment is the complex treatment of Chilling (7 and 14 days- 4˚C) and 500 ppm gibberellic acid which increased germination percentage to about 84% and also germination velocity to a high rate.


Akbari, G.H.A., Modarres Sanavy, S.A.M. and Yousefzadeh, S. 2007. Effect of auxin and salt stress (NaCl) on seed germination of wheat cultivars (Triticum aestivum L.). Pakistan Journal of Biological Sciences, 10(15): 2557-2561. (Journal)
Ahmadi, H. 1996. Applied geomorphology (erosion). University of Tehran Press, 706p. )In Persian)(Book)
Baskin, C.C. and Baskin, J.M., 1991. Nondeep complex morphophysiological dormancy in seeds of Osmorhiza ckiytonii (Apiaceae). American Journal of Botany, 78: 588-593. (Journal)
Baskin, C.C., Chester, E.W. and Baskin, J.M. 1992. Deep Complex morphological dormancy in seeds of Thaspium pinnatifidum. International Journal of Plant Science, 153: 565-571. (Journal)
Baskin, C.C., Baskin, J.M. and Chester, E.W., 1999. Seed dormancy in the wetland winter annual Pitilimnium nuttallii (Apiaceae). Wetlands, 19: 359-364. (Journal)
Baskin, J.M. and Baskin, C.C. 2004. A classification system for seed dormancy. Seed Science Research, 14: 1-16. (Journal)
Bello, I.A., Hatteiman-Valentini, H. and Owen, M.D.K. 1998. Effects of stratification, temperature and oxygen on woolly cupgrass (Eriochloa villosa) seed dormancy. Weed Science, 46: 526-529. (Journal)
Cheraghi, F. 2010. Evaluation of treatments to break dormancy and seed priming on germination and plant establishment medicinal Angelica (Heracleum persicum Desf). Seed Science and Technology Thesis. University of Birjand. )In Persian)(Thesis)
Ellis, R.A. and Roberts, E.H. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9: 373-409. (Journal)
Ehyaie, H. and Hoseyni, M. 2011. Evaluation of sleep characteristics of germination and seed mass in medicinal plants. Iranian Journal of Crop Science, 9(4): 651-658. )In Persian)(Journal)
Garcia-Gusano, M., Martýnez-Gomez, P. and Dicenta, F. 2004. Breaking seed dormancy in almond (Prunus dulcis (Mill.) D.A. Webb). Scientia Horticulturae,99: 363.370. (Journal)
Gangjou barzelabadi, M. and Tavakoli, M. 2008. Seed germination of 10 desert plant species studied. Range and Desert, 15(2): 215- 226. )In Persian)(Journal)
Ghasemi pirbalooti, A., Gol parvar, A., Riahi dehkordi, M. and Navid, A. 2007. The effect of different treatments on breaking of dormancy and stimulate seed germination of five species of medicinal plants in Chaharmahal and Bakhtiari. Research and Construction, 4(74): 185-192. )In Persian)(Journal)
Ghorbani, S., Khaje hoseyni, M. and Eishi rezaei, A. 2013. The effects of treatments on the germination and early growth levels of humic acid (Zea mays L.). Agronomy Journal, 6(2): 37-43. )In Persian)(Journal)
Haghighikhah, M. and Khaje hoseini, M. 2013. Different methods for breaking dormancy on germination of seeds of barnyard grass species Echinochloa orizy cola and Echinochloa crus galli. Plant Protection, 27(2): 255-257. )In Persian)(Journal)
Hojjati, Y., Naderi, R.A., Faramarzi, A. and Gholipur, G. 2007. Check sulfuric acid and gibberellic acid treatments and temperature on seed germination Cycas (Cycas revolute L.). Journal of New Agricultural Science. 3(9): 14-20. )In Persian)(Journal)
Iglesias, R.G. and Babiano, M.J. 1997. Endogenous abscisic acid during the germination of chickpea seed. Physiologia Plantarum, 100: 500-504. (Journal)
Karam, N.S. and Al-salem, M.M. 2001. Breaking dormancy in Arbutus andrchne L. seeds by stratification and gibberelic acid. Seed Science and Technology, 29: 52-56. (Journal)
Karimi, A. 2001. The nutritive value of artichoke (Gundelia tournefortii) treated with SO2 gas and its use in the diet of fattening lambs. )In Persian)(Thesis)
Khan, A.A., 1971. Cytokinins: Permissive Role in Seed Germination. Science, 171: 853-859. (Journal)
Khatamian, N., Nabavi kalat, S.M. and Bakhsh klahrestani, K. 2011. Effects of humic acid biological characteristics of triticale grain yield (Tritico secule (Wittmack). First National Conference on new issues in agriculture, Islamic Azad University, Saveh Branch, Saveh, Iran. )In Persian)(Conference)
Koo, E.S. 2006. Humic acid or fulvic acid: which organic acid accelerates the germination of the green mung beans. California State Science Fair. 1617. (Handbook)
Mehanna, H.T., Martin, G.C. and Nishyama, C., 1985. Effects of temperature, chemical treatments and endogenous hormone content on peach seed germination and subsequent seedling growth. Scientia Horticulturae, 27: 63-73. (Journal)
Mohammadi chiane, S., Alizade, M. and Hasani, A. 2011. The effect of the stratification and thermal shock break dormancy and stimulate germination of seeds of medicinal plants Kygr (Gundelia tournefortii). First National Congress of the New Agricultural Science and Technology. University of Zanjan, Zanjan, Iran. )In Persian)(Conference)
Parmenter, G.A., Burton, L.C. and Littlejohn, R.P., 1996. Chilling requirement of commercial Echinacea seeds. New Zealand Journal of Crop and Horticultural Science, 24: 109-114. (Journal)
Piccolo, A., Celano, G. and Pietramellara, G. 1993. Effects of fractions of coal-derived humic substances on seed germination and growth of seedlings (Lactuca sativa and Lycopersicon esculentum). Biology and Fertility of Soils, 16(1): 11-15. (Journal)
Phillips, N., Drost, D. and Varga, W. 2003. Chemical treatments enhanced seed germination in Perkleridia gairdneri. Acta Horticulturae, 618: 477-482. (Journal)
Rezaei chiane, A., Tajbakhsh, M., Valizadegan, A., Banaei asl., F. and Mahdavi kia, H. 2014. Effective methods for breaking dormancy in Bzralbnj Grid (Hyoscyamus reticulatus L). Journal of Agricultural Research in Iran, 12(2): 246-253. )In Persian)(Journal)
Sabzevari, S., Khazaei, H.R. and Kafi, M. 2010. Effects of humic acid on germination of winter wheat cultivars (Sayvnz and Sabalan) and spring (Chamran and leading). Journal of Agricultural Research, 8(3): 473-480. )In Persian)(Journal)
Sasani, S., Tavakol Afshar, R., Puostini, K. and Sharif zadeh, F. 2005. The effects of hormonal treatments and storage period on seed dormancy and germination of seeds of caraway. Iranian Journal of Agricultural Sciences, 2; 287-294. )In Persian)(Journal)
Schmitz, N., Xia, G.H. and Ketmode, A.R. 2001. Dormancy of yallow Ceder seeds is retminated by gibberlic acid in combination with fluridone or with osmotic priming and moist chihhing. Seed Science and Technology, 29: 331-346. (Journal)
Shams Esfandabadi, R.F., Shariati, M. and Modares Hashemi, S.M. 2005. Check out some of dormancy treatments in five seed populations of bearded steppe. (Stipa barbata Desf.) Iranian Journal of Biology, 18(1): 48-59. )In Persian)(Journal)
Tavili, A., Zare, S. and Yari, R. 2010. The effect of different treatments on seed dormancy and germination stimulation Mvdndrvn (Ammodendron persicum). Range and Desert, 3(40): 466-475. )In Persian)(Journal)
Turkman, O., Dursun, A., Turan, M. and Erdinc, C. 2004. Calcium and humic acid affect seed germination, growth, and nutrient content of tomato. Soil and Plant Science, 54: 168-174. (Journal)
Tan, K.H. 2003. Humic Matter in Soil and the Environment. Marcel Dekker, New York. (Book)
Zanguei, M. and Parsa, S. 2015. The effect of seed dormancy breaking methods coma. Journal of Eqohidrology Seed, 1(1): 17-28. )In Persian)(Journal)