To Compare the priming and pre-chilling treatment on seed germination, vigor and seedling appearance improvement in some populations of Tripleurospermum sevanense species

Document Type : Research Paper


1 Associate Professor, Research Institute of Forests and Rangeland, Education and Extension Organization (AREEO), Karaj, Iran

2 MSc Gratuated of Seed Science and Technology, College of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

3 Associate Professor, College of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

4 Assistant Professor, Research Institute of Forests and Rangeland, Education and Extension Organization (AREEO), Karaj, Iran


Pseudo Chamomile (Tripleurospermum sevanense) is medicinal and aromatic plant. This plant is growing in natural habitats of Iran. In this study, emergence enhancement of seed and seedlings vigor of three populations, were compared with priming technique and pre-chilling treatment. A factorial experiment based on randomized complete design with three replication and six treatment was conducted in laboratory (2010) and greenhouse (2011) in Research Institute of Forests and Range Lands. The priming treatments including: [GA3 (125 and 250ppm), KNO3 (0.5 and 1%), distilled water (control)] and moist chilling at 4°C. In laboratory experiment, the results of treatments on seed germination characteristics of 17952-Fars population showed that maximum germination (96%), fresh weight (97.33 mg) were obtained with effect of potassium nitrate. With effect GA3, speed of germination (18.7 sprout/day) Vigor index (127.91) were more than control and other treatments, meanwhile in greenhouse experiment, effect of treatments on seedling appearance characteristics of above population showed that potassium nitrate  treatment had  more significant effect on seedling appearance percentage (85.55%), seedling length(103/46mm), seedling vigor index (88.35) control and other treatments. On the base of results, it is recommended for cultivation and domestication of medicinal species, especially in spring, priming technique would e used for uniform germination and seedling appearance.


Abdul-baki, A.A. and Anderson, J.D. 1973. Vigour determination in soybean seed by multiple criteria. Crop Science, 13: 630-633. )Journal)
Albeles, F.B. and Lonsilk, J. 1996. Stimulation of lettuce seed germination by ethylene. PlantPhysiology, 44: 277-280. )Journal)
Amri, E. 2001. Germination of Terminalia sericea Buch. Ex Dc seeds: Effects of temperature regime, photoperiod, gebberellic acid and potassium nitrate. InternationalJournal of Application. Biology andPharmacologistTechnology, 2(2): 104-110. )Journal)
Anonymous, 2008. International rules for seed testing. International seed testing association (ISTA). Switzerland. )Handbook)
Arefi, I.H., Khani Nejad, S. and Kafi, M. 2012. Roles of duration and concentration of priming agents on dormancy breaking and germination of caper (Capparis spinosa) for the protection of arid degraded areas. Pakistan Journal of Botany, 44: 225-230. )Journal)
Farooq, M., Basra, S.M.A., Tabassum, R. and Ahmad, N. 2006. Evaluation of seed vigour enhancement techniques on physiological and biochemical techniques on physiological basis in coars rice (Oryza sativa L.). Seed Sciences and Technology, 34: 741-750. )Journal)
Farooq, M., Basra, S.M.A., Afzal, I. and Khaliq, A. 2006. Optimization of hydropriming techniques for rice seed invigoration. Seed Sciences and Technology, 34: 507-512. )Journal)
Hartmann, H.T., Kester, D.E., Davies, F.J. and Geneve, R.L. 1997. Plant propagation principles and practices. PlantPhysiology, 3: 104-110.)Journal)
Glesias, R.G. and Babiano, M.J. 1997. Endogenous abscisic acid during the germination of chickpea seed. Physiology Planta, 100: 500-504. )Journal)
International Seed Testing, 2007. The 28th ISTA Congress, April 2007, Brazil, No.133. Rules Proposals for International Seed Testing, 2011. Rules Proposals for the International Rules for Seed Testing, 2011 Edition, Appendix 1: Revised Table 5A, Chapter 5: Germination, 5-56. )Handbook)
Lekh, R. and Khairwal, I.S. 1993. Evaluation of pearl millet hybrids and their parents for germ inability and field` emergence. Indian Journal of Plant Physiology, 2: 125-127. )Journal)
Maguire, J.D. 1962. Speed of germination: aid in selection and evaluation for seedling vigour. CropScience, 2: 176-177. )Journal)
Mozaffarian, V. 2008. Flora of Iran, Asteraceae (Compositae): Tribes Anthemideae and Echinopeae, Institute of Forests and Rangelands Press, First edition, 59: 169. (In Persian))Journal)
Nadjaf, M., Banayan, L., Tabrizi, I. and Rastgoo, M. 2006. Seed germination and seed dormancy breaking techniques for Ferula gummosa and Teucrium polium. Journal of Arid Environments, 30: 508-512. )Journal)
Nasar Alipor, A. and Sefidkon, F. 2003. Quantative and qualitative study of essential oil from aromatic and medicinal plant Tripleurospermum dyscforme (C.A.Mey.) Schultz-Bip. Journal of Medicinal Plants, 2(6): 33-40. )Journal)
Nascimento, W.M. and Aragao, F.A.S. 2004. Muskmelon seed priming in relation to seed vigor. ScienceAgricola, 61(1): 114-117. )Journal)
Rouhi, H.R., Rahmati, H., Saman. M., Shahbodaghloo, A.R., Karimi, F.A., Moosavi, S.A., Rezaei, M.E. and Karimi, F. 2012. The effect of different treatments on dormancy-breaking of Galbanum seeds (Ferula gummosa Boiss). InternationalJournal ofAgriculturalSciences, 2(7): 598-604. )Journal)
Shariati, M., Asmaneh, T. and Moddares Hashemi, M. 2001. Evaluation of different treatment for seed breaking dormancy of Achilla, Pajohesh and Sazandegi, (56, 57): 2-8. )Journal)
Shekary, F., Baljani R., Saba, J., Afsahi, K. and Shekari, F. 2010. Effect of seed priming with salicylic acid on growth characteristics of borage plants (Borago officinalis) seedlings. Journal of New Agricultural Science (Modern Science of Sustainable Agriculture), 6(18): 47-53. )Journal)
Tavili, A., Safari, B. and Saberi, M. 2010. Effect comparing by using of gibbelic acid and potasim nitrat on seed enhancement germination characteristics of Salsola rigida. Rangeland Desert, 2: 272-280. )Journal)
Varner, J.E. 1964. Gibberlic acid controlled synthesis of α-amylase in barley endosperm. Plant Physiology, 39: 413-415. )Journal)