Effect of MS and N6 media and auxin and cytokinin hormones on regeneration of bread wheat lines from coleoptile explants

Document Type : Research Paper

Authors

Department of Agriculture Biotechnology, Azarbaijan Shahid Madani University, Tabriz

Abstract

Optimizing tissue culture for the gene transfer process and the preparation of plants free from bacterial and viral diseases is necessary. This experiment was conducted as factorial in a completely randomized design with 3 replications in the Biotechnology Laboratory of the agricultural faculty of Azarbaijan Shahid Madani university in 2016. In wheat Various explants such as inflorescences, mature embryos and adult embryos have been used for tissue culture. One of the major disadvantages of monocots plants is that when the adult embryo is used as an explant, the frequency of regeneration of callus decreases in comparison with immature embryos. In this experiment, for wheat regeneration was used two different media: MS and N6, and different auxin and cytokines hormones. The results showed that the best genotype for regeneration was CD-9 and among different cytokine hormones, the best was BAP hormone containing 2 mg / L, and among different hormones of auxin, the highest regeneration obtained in the medium containing 1 mg / L IAA with BA 1mg / L. In MS culture medium, the highest regeneration rate was observed for MS(3) medium  and in N6 medium, regeneration was highest for N6(3). Comparison of MS medium with N6 in regeneration of wheat cultivars showed that MS medium is better.

Keywords

References

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Iranian Journal of Seed Sciences and Research
Volume 6, Issue 2
September 2019
Pages 215-227

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