Evaluation of seed origin, storage and seed treatment on seed quality analysis and biochemical of hybrid corn (cv. SC.704)

Document Type : Research Paper

Authors

Seed and Plant Certification and Registration Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

This experiment was conducted to evaluate the changes in deterioration of two different sources of seed corn in two different conditions. Treatments were two seed origins production (Karaj and Moghan), seed storage in two regions (controlled warehouse& Warehouse in Moghan climate), seed asepsis at two levels (Aseptic&  non-aseptic). Experiment was done as a factorial experiment based on completely randomized design with three replications. Measured characters included: normal seedlings percentage, mean time germination, seedling length and weight vigour indices, normal seedlings percentage in cold test, radical emergence percentage, catalase and peroxidase activities and malondialdehyde contents. Results illustrated seeds produced in Karaj compared to Moghan 4 %, maintained seeds in controlled warehouse compared to non-controlled 3%, and aseptic seeds compared to non-aseptic 2 % had more normal seedlings. Catalase activity in seeds produced in Karaj, were substantially higher (14 %) than the same seeds in Moghan. Seed treatment had no significant effect on catalase activity. Seed treatment had significant effect on peroxidase activities and malondialdehyde contents. peroxidase activity in aseptic seeds was about 11% higher and malondialdehyde was about 8 % lower than non-aseptic seeds. Interaction of seed source and seed storage on normal seedlings percentage in cold test, Peroxidase activity and malondialdehyde contents were substantially considerable. It means seeds produced in Karaj and maintained in controlled warehouse had the highest percentage in normal seedlings (92 %), Peroxidase activity (0.042 umol H2o2/min/mg protein) and had the lowest rate of malondialdehyde (3 nmol/gfresh weight). Thus the reducing of antioxidant enzymes activity leads to disruption of germination, vigour and seed deterioration.

Keywords

References

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Iranian Journal of Seed Sciences and Research
Volume 5, Issue 2
July 2018
Pages 109-121

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