The effect of drought stress on some morphological and physiological characters in canola seedling (Brassica napus)

Document Type : Research Paper

Authors

member of Scientific board/ University of Guilan

Abstract

In order to study some morphological and physiological traits of susceptible (Hayola308) and tolerant (SLM046) genotypes of canola to drought stress, an experiment was conducted in CRD design with factorial split in time arrangement. The applied factors were genotype (susceptible and tolerant) and irrigation condition (non-stress and stress). The stress was applied at before flowering time and the traits were measured at 24, 48, 72 and 96 hours after it in both condition. The result showed a reduction in dry weight of root in Hayola308 genotype, and a significant increase in root to shoot length ratio, fresh weight of root and malondialdehyde level (MDA) in SLM046 genotype. The 48 hours after drought stress, the fresh weight of shoot in SLM046 genotype was higher than Hayola308 genotype. Relative water content (RWC) in SLM046 and Hayola308 at 24 hours after stress were 88.59% and 38.52%, respectively. In Hayola308 genotype, hydrogen peroxide (H2O2) content in stress condition was higher than non-stress ones. There was not a significant difference among studied treatments for electrolyte leakage. In general, the increase of root length, root to shoot length ratio, RWC, fresh weight of root and the difference between fresh and dry weight of root and shoot and no change in dry weight of root and H2O2 content in SLM046 genotype indicated that this genotype had traits which could assign tolerance to drought stress.

Keywords


Anjum, S. A., Xie, X. Y., Wang, L. C., Saleem Ardalani, SH., Saeidi, M., Jalali Honarmand, S., Eghbal Ghobadi, M. and Abdoli, M. 2015. Effect of post anthesis drought stress on some agronomic and physiological traits related to source strength in four bread wheat genotypes. Journal of Cereal Research. 5(1): 45-65. (Journal)
 
Barzana, G., Aroca, R., Bienert, G. P., Chaumont, F. and R. Lozano, G. M. 2014. New insights into the regulation of aquaporins by the arbuscular mycorrhizal symbiosis in maize plants under drought stress and possible implications for plant performance. Journal of the American Phytopathological Society. 27(4): 349–363. (Journal)
Cui, L. L., Lu, Y. S., Li, Y., Yang, C. and Peng, X. X. 2016. Overexpression of glycolate oxidase confers improved photosynthesis under high light and high temperature in rice. Journal of Frontiers in Plant Science. 7(1165): 1-12. (Journal)
Davey, M. W., Stals, E., Panis, B., Keulemans, J. and Swennen, R. L. 2005. High-throughput determination of malondialdehyde in plant tissues. Journal of Analytical Biochemistry. 347(2): 201-207. (Journal)
Fathi, GH. Enayat-gholizadeh, M. 2011. The effect of drought stress in growth stages on seed and oil yield of canola. Crop physiology journal. 2(8): 97-114. (Journal)
Garc─▒´a-Mata, C. and Lamattina, L. 2001. Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress. Journal of American Society of Plant Biologists. 126: 1196–1204. (Journal)
Hamed, A., Akbari, Gh. A., Khoshkholqsima, N. A., Shiranirad, A. H., Jabbari, H. and Tabatabaee, S. A. 2014. Evaluation of the agronomic characteristics and some physiological traits of canola varieties under drought stress. Journal of Environmental Stresses in Crop Sciences. 7(2): 155-171. (In Persian) (Journal)
Hatamvand, M., Hasanloo, T., Dehghannayeri, F., Shiranirad, A. H., Tabatabaee, S. A. and Hosseini, S. M. 2014. Evaluation of some physiological and biochemical indices of canola cultivars in response to drought stress. Journal of Environmental Stresses in Crop Sciences. 7(2): 173-185. (In Persian) (Journal)
Heath, R. L. and Packer, L. 1968. Photoperoxidation in isolated chloroplasts: I. kinetics and stoichiometry of fatty acid peroxidation. Journal of Archives of biochemistry and biophysics. 125(1): 189-198. (Journal)
Huang, Q., Zhao, Y., Liu, C., Zou, X., Cheng, Y., Fu, G., Xu, J., Zhang, X. and Lu, G. 2015. Evaluation of and selection criteria for drought resistance in Chinese semiwinter rapeseed varieties at different developmental stages. Journal of Plant Breeding. 134(5): 542-550. (Journal)
Husen, A., Iqbal, M. and Aref, I. M. 2014. Growth, water status, and leaf characteristics of Brassica carinata under drought and rehydration conditions. Brazilian Journal of Botany. 37(3): 217-227. (Journal)
Junglee, S., Urban, L., Sallanon, H., Félicie, L. and Lauri, F. 2014. Optimized assay for hydrogen peroxide determination in plant tissue using potassium iodide. American Journal of Analytical Chemistry. 5: 730-736. (Journal)
Masinde, P. W., Stutzel, H., Agong, S. and Goand- Fricke, A. 2006. Plant growth, water relations and transpiration of two species of african nightshade (Solanum villosum Mill. ssp. Miniatum (Bernh. Ex Willd.) Edmonds and S. sarrachoides Sendtn.) Under water-limited conditions. Journal of Scientia Horticulturae. 110: 7-15. (Journal)
Mirzaee, M., Moieni, A. and Ghanati, F. 2013. Effects of drought stress on the lipid peroxidation and antioxidant enzyme activities in two canola (Brassica napus L.) cultivars. Journal of Agricultural Science and Technology. 15(3): 593-602. (In Persian) (Journal)
Mohammadi, N., Baghizadeh, A. and Rajaee, P. 2015. Effect of β-amino buthyric acid (BABA) on relative leaf water content, osmotic adjustment and antioxidant enzymes in rapeseed (Brassica napus L.) plants under drought stress. Journal of Plant Researches (Iranian Journal of Biology). 28(4): 844-860. (In Persian) (Journal)
Mohsenzadeh-Golfazani, M., Mohammad, F., Hasani-Kumleh, S.H., Samizadeh-Lahiji, H. 2016. Grouping of some canola genotypes in various drought stress treatment in germination stages based on multivariate statistical methods. Journal of Seed Science and Research. 3(2): 53-65. (In Persian) (Journal)
Nasiri, M., Meskarbashi, M., Hassibi, P. and Pirdashti, H. 2015.Screening of rice genotypes by some morphological and physiological traits under drought stress condition. Journal of Plant ProductionResearch.22(2): 95-117. (In Persian) (Journal)
Ozden, M., Demirel, U. and Kahraman, A. 2009. Effects of proline on antioxidant system in leaves of grapevine (Vitis vinifera L.) exposed to oxidative stress by H2O2. Journal of Scientia Horticulturae, 119(2): 163-168. (Journal)
Razaviezadeh, R. and Amoubeigi, M. 2013. The effect of drought stress and paclobutrazol on the accumulation of flavonoids and minerals in vitro in canola (Brassica napus). Journal of Iranian Plant Ecophysiological Research. 31(3): 12-22. (In Persian) (Journal)
Sairam, R. K., Rao, K. V. and Srivastava, G. C., 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Journal of Plant Science. 163(5): 1037-1046. (Journal)
Sánchez-Rodríguez, E., Rubio-Wilhelmi, M., Cervilla, L. M., Blasco, B., Rios, J. J., Rosales, M. A., Romero, L. and Ruiz, J. M., 2010. Genotypic differences in some physiological parameters symptomatic for oxidative stress under moderate drought in tomato plants. Journal of Plant Science. 178(1): 30-40. (Journal)
Seyed-ahmadi, A., Gharineh, M. H., Bakhshandeh, A., Fathi, Gh. and Naderi, A. 2012. Study of terminal drought stress on yield, yield components oil and protein, percentage and root growth canola under ahvaz climate conditions. Journal of the Plant Production (Agronomy, Breeding and Horticulture). 34(2): 53- 66. (In Persian) (Journal)
Shaabani, A., Kamgarhaghighi, A., Spaskhah, A., Emami, Y. and Honar, T. 2009. Effect of water stress on physiological parameters of oil seed rape (Brassica napus). Journal of Water and Soil Science (Journal of Science and Technology of Agriculture and Natural Resources). 13(49): 31-42. (In Persian) (Journal)
Sharp, R. 2002. Interaction with ethylene: changing views on the role of abscisic acid in root and shoot growth responses to water stress. Journal of Plant, Cell and Environment. 25l: 211-222.(Journal)
Shiferaw, B. and Baker, D. A., 1996. An evaluation of drought screening techniques for Eragrostis tef. Journal of Tropical Science, 36: 74-85. (Journal)
 
Thomas, C., Alcock, T., Graham, N., Hayden, R., Matterson, S., Wilson, L., Young, S., Dupuy, L., White, P., Hammond, J., Danku, J., Salt, D., Sweeney, A., Bancroft, I. and Broadley, M. 2016. Root morphology and seed and leaf ionomic traits in a Brassica napus L. diversity panel show wide phenotypic variation and are characteristic of crop habit. Journal of Bio Med Central Plant Biology, 16: 214-232. (Journal)
Türkan, ─░., Bor, M., Özdemir, F. and Koca, H. 2005. Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Journal of Plant Science. 168(1): 223-231. (Journal)
Turner, N. C. 1981. Techniques and experimental approaches for the measurement of plant water status. Journal of Plant and soil. 58(1): 339-366. (Journal)
Wu, Y. and J. Cosgrove, D. 2000. Adaptation of roots to low water potentials by changes in cell wall extensibility and cell wall proteins. Journal of Experimental Botany. 51(350): 1543- 1553. (Journal)
Zhao, L. Y., Wang, W. and Song, Y. W. 2010. Changes of photosynthesis and membrane damage of Brassica napus under soil water stress. Journal of Henan Agricultural Sciences. 8: 33-35. (Journal)