Effect of copper, nickel and cobalt stress on tuber germination traits of Cyperus rotundus under in vitro conditions

Document Type : Research Paper

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Abstract

In order to evaluate cyperus rotundus resistance to heavy metals stress, copper, nickel and cobalt, in the germination stage, the experiment was conducted in factorial based on completely randomized design with three replications in agronomy and horticulture laboratory of Jiroft University. The first factor consists of three metal copper, nickel, cobalt and the second factor levels consists of metal concentrations (0, 200, 400, 600, 800, 1000 ppm), respectively. The results showed that germination percentage, rate and mean germination time were affected significantly (p <0.01) affected by heavy metals and levels of stress and only, germination percentage were affected by their interactions. The effect of stress levels showed that treatments of 200, 400, 600, 800 and 1000 ppm, compared to control the tubers germination rate decreased tubers 17.8%, 26.07%, 35.7%, 46.4% and 57.1%, respectively. These treatments decreased tubers germination percentage respectively 9.2%, 14.9%, 24.1%, 36.7% and 45.9% compared to the control. The lowest germination percentage (70.5) and rate (1.7) happened in nickel stress. The Regression analysis results showed that germination rate changes affect the stress levels of copper, nickel and cobalt fit on quadratic function with R- square (R2) above 90%, which was statistically significant at 0.01. In general, the results of this experiment showed cyperus rotundus is tolerance to heavy metals stress so that, even able to germination and growth at concentrations up to 1000 mg/L, also tolerance Capability cyperus rotundus to copper heavy metal was higher than cobalt and nickel i.e. Cu >Co >Ni.

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References

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Iranian Journal of Seed Sciences and Research
Volume 4, Issue 1
June 2017
Pages 37-48

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