Evaluation of germination factors, antioxidant enzymes and electrical conductivityof pinto bean (Phaseolus vulgaris L) seeds obtained from the pre-treatment of the m other plant seeds

Document Type : Research Paper

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Abstract

Due to the role seeds germination of the plant establishment, optimal germination as a key factor in agriculture is important. In order to determine the components of germination, enzymes, antioxidants and electrical conductivity seeds obtained from pretreatment a mother plant seed on the farm a factorial experiment in a completely randomized design with three replications was conducted in the laboratory of Bio-center, College of Agriculture, Zabol University. first factor include varieties at 3 levels (Pinto bean varieties E9, E10, and Khomain) and second factor seeds obtained from pretreatment the mother plant seed on the farm at 7 levels: 1-(polyethylene glycol (PEG) -5 bar at 6 hours, 2- salicylic acid (C7H6O3) 500 mg per liter at 12 hours, 3- potassium chloride (Kcl) -20 mM at 6 hours, 4-calcium chloride( Cacl2) - 15 mM at 3 hours, 5- sodium chloride (Nacl) -20 mM at 6 hours, 6-  distilled water at 12 hours and 7- without pretreatment (control). Percentage and rate germination, weight and lengh vigor index, energy germination 4th days, Alpha-amylase, Sugar solution, Peroxidase, Peroxidase and super oxide despotize were measured. The result showed that the interaction between pretreatment and varieties on germination traits include germination percentage, weighting vigor index, Energy Germination 4th days at 1% and vigor index length at 5% were significant. The biochemical characteristics of seeds obtained from pretreatment the mother plant seed on the farm was significant at 1%. The highest percentage and germination rate were obtained calcium chloride at 3 hours varieties E9, highest vigor index on calcium chloride at 3 hours varieties E10 and soluble sugar, alpha-amylase, peroxidase on calcium chloride at 3 hours.  The result showed pretreatment Cacl2 at 3 hours improved the germination and growth seedling on bean. Pretreatment seeds of mother plant are good germination and according to variety and selection of appropriate pretreatment for mother plant, can improve germination, establishment and growth seedling beans

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References

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Iranian Journal of Seed Sciences and Research
Volume 3, Issue 4
January 2017
Pages 53-65

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