Effect of salicylic acid pre-treatment on cucumber and watermelon seeds germination under salt stress

Document Type : Research Paper

Authors

Abstract

Salinity is one of the most important environmental stresses that affect the growth and yield of plants. To study the effect of salinity and salicylic acid on germination of cucumber (Cucumis sativus cv. Super Dominus) and watermelon (Citrullus lanatus cv. Crimson Sweet) seeds, two factorial experiments were conducted in a completely randomized design with three replications. Treatments consisted of four levels of salinity (0, 2, 4, 6 ds/m sodium chloride) and three levels of salicylic acid (0, 0.5, 1 mM). The results showed that salinity significantly reduced seed germination and seedling growth. The lowest values of germination rate in cucumber (18.79) and watermelon (10.33), germination percentage in cucumber (86.65 percent) and watermelon (69.63) and seed vigor index in cucumber (17.93) and watermelon (9.59) was observed in 6 ds/m level of salinity. Salicylic acid improved germination parameters in both plant. The highest seed germination (97.98 percent) was obtained in the 1 mM level of SA under control condition. According to the results, pretreatment of cucumber and watermelon seeds by 1 mM SA can be proposed to improve seed germination and growth rate of seedling under salinity.

Keywords


Abdul-Baki, A.A. and Anderon, J.D. 1973. Vigor determination in soybean by multiple criteria. Crop Science, 13: 630-633. (Journal)
Alebrahim, M.T., Sabaghnia, N., Ebadi, A. and Mohebodini, M. 2004. Investigation the effect of salt and drought stress on seed germination of thyme medicinal plant (Thymus vulgaris). Journal Research in Agriculture Science, 1: 13-20. (Journal)
Alirezayi Naghdar, M., Azizi, M. and Valizade Gholbeik, A. 2012. Effect of salinity on seed germination and seedling growth characteristics of four cultivars of medicinal basil. Science and Seed Technology, 2(4): 1-13. (In Persian)(Journal)
Anon, A. 1978. United states standards for grades of watermelons. USDA Washington. (HandBook)
Ashraf, M. and Waheed, A. 1990. Screening of local exotic of lentil (Lens culinaris Medik) for salt tolerance at two growth stage. Plant and Soil, 128: 167-176. (Journal)
Baghani, J., Alizade, A., Ansari, H., Azizi, M. and Sadeghian, H. 2013. The effect of irrigation with salty water melon phenological characteristics. Journal of Irrigation and Drainage. 7(2): 222-230. (In Persian)(Journal)
Baghbani, A., Forghani, A.H. and Kadkhodayi, A. 2013. Study of salinity stress on germination and seedling growth in greenhouse cucumber cultivars. Journal of Basic and Applied Scientific Research, 3(3): 1137-1140. (Journal)
Bagheri Kazem Abadi, E., Sarmadnia, Gh. and Hajrasoli, Sh. 1997. Reply sowing population to drought and salinity at germination stage. Journal of Agricultural Science and Technology, 2: 55-41. (In Persian)(Journal)
Basra, S.M.A., Afzal, I., Rashid, R.A. and Hameed, A. 2005. Inducing salt tolerance in soybean by seed vigor enhancement techniques. Journal of Biotechnology and Biochemical, 1: 173-179. (Journal)
Ehteshamnia, E. 2007. Report on the latest approaches salinity on seedling growth of 10 medicinal plant components. Proceedings of the Third Symposium of Medicinal Plants. Shahed University. Tehran. Pp: 123. (Conference)
El-Keblawy, A. and Al-Rawai, A. 2005. Effects of salinity, temperature and light on germination of invasive Prosopis juliflora. Journal Arid Environment, 61: 555-565. (Journal)
El-Tayeb, M.A. 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation, 45: 215-225. (Journal)
Epstein. E., Norlyn, J. and Rush, D.W. 1980. Saline culture of crops: a genetic approach. Science, 210: 399-404. (Journal)
Fariduddin, Q., Hayat, S. and Ahmad, A. 2003. Salicylic acid influences net photosynthetic rate, carboxylation efficiency, and nitrate reeducates activity and seed yield in Brassica juncea. Photosynthetic, 41(2): 281-284. (Journal)
Grieve, C.M., Lesch, S., Francois, L.E. and Maas, E.W. 1992. Analysis of main-spike yield components in salt stressed wheat. Crop Science, 32: 697-703. (Journal)
Hanan, E.D. 2007. Influence of salicylic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare. Biological Research, 1: 40- 48. (Journal)
Harris, D. 1996. The effects of manure, genotype seed priming, depth and date of sowing on the emergence and early growth Sorghum bicolor. Soil Tillage Research, 40: 73-88. (Journal)
Kafi, M. and Stovart, S. 1997. The effects of salinity on growth and yield of nine wheat cultivars. Journal of Agricultural Science and Technology, 12: 76-85. (In Persian)(Journal)
Katergi, N., Van Hoorn, J.W., Hamdy, A., Karam, F. and Mastrortilli, M. 1994. Effect of salinity on emergence and on water stress early seedling growth of sunflower and maize. Agricultural Water Management, 26: 81-91. (Journal)
Khodary, S.E.A. 2004. Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt-stressed maize plants. International Journal of Agriculture Biology. 6: 5–8. (Journal)
Krishramurthy, L., Ito, O., Johansen, C. and Saxsena, N.P. 1998. Length to weight ratio of chickpea roots under progressively reducing soil moisture conditions in a vertical. Field Crops Research, 58: 177-185. (Journal)
Lechno, S., Zamski, E. and Tel-Or, E. 1997. Salt stress induced responses in cucumber plants. Journal of Plant Physiology, 150: 206-211. (Journal)
Livington, N.J. and De Jong, E. 1990. Matrix and osmotic potential effects on seedling emergence at different temperatures. Agronomy Journal, 82: 995-998. (Journal)
Lynch, J. and Lauchli, A. 1988. Salinity affects intracellular calcium in corn root protoplasts. Plant Physiology, 87: 351-356. (Journal)
Maguire, J.D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2: 176-177. (Journal)
Mayer, A.M. and Polijakoff-Mayber, A. 1989. Effect of salinity on emergence and on water stress early seedling growth of sunflower and maize. Agricultural Water Management, 26: 81-91. (Journal)
Postchi, A. 1971. Working kitchen garden. Franklin Institute. Tehran. Pp: 330. (In Persian)(Handbook)
Postini, K. and Zehtab Salmasi, S. 1997. The effect of salinity on the production and dry matter remobilization in two wheat cultivars. Iranian Journal of Field Crop Science, 29(4): 11-16. (In Persian) (Journal)
Puppala, N., Poindexter, J.L. and Bhardwaj, H.L. 1999. Evaluation of salinity tolerance of canola germination. Perspectives on new crops and new uses. ASHS Press, Alexardria, pp. 251–253. (Handbook)
Raskin, I. 1992. Role of salicylic acid in plants. Annual Review of Plant Physiology. 43: 439-463. (Handbook)
Redmann, R.E., Ql, M.Q. and Belyk, M. 1994. Growth of transgenic and standard canola (Brassica napus L.) varieties in response to soil salinity. Plant Science, 74: 797-799. (Journal)
Rehman, S., Harris, P.J.C., Bourne, W.F. and Wikin, J. 1997. The effects of sodium chloride on germination, potassium and calcium contacts of Acacia seeds. Seed Science and Technology, 25: 45-57. (Journal)
Rezayi, M. 2004. Report on the latest approaches salinity on seed germination and cumin. Conference abstracts collection of medicinal plants. Shahed University, Tehran, Pp: 93. (In Persian)(Thesis)
Ruan, S. 2002. The influence of priming on germination of rice seeds and seedling emergence and performance in flooded soil. Seed Science and Technology, 30: 61-67. (Journal)
Sahabutdinova, D.R. and Shakirova, F.M. 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science, 164: 317-322. (Journal)
 
Senaratna, T., Touchell, D, Bunn, E. and Dixon, K. 1999. Acetyl salicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulation, 30: 157-161. (Journal)
Senaratna, T., Bunn, E. and Bishop, A. 2002. Triazole treatment of explants source provides stress tolerance in progeny of geranium (Pelargonium hortorum Bailey) plants regenerated by somatic embryogenesis. Plant Growth Regulation, 36(2): 169-174. (Journal)
Serraj, R. and Sinclair, T.R. 2002. Osmolyte accumulation: can it really help increase crop yield under drought conditions. Plant cell Environment, 25: 333-341. (Journal)
Sharikova, F., Sakhabutdinova, A., Bezrukova, M., Fatkhutdinova, R. and Fatkhudinova, D. 2003. Changes in the hormonal status of wheat seedling induced by salicylic acid and salinity. Plant Science, 164: 317-322. (Journal)
Sivritepe, N., Sivritepe, H.O. and Eris, A. 2003. The effects of NaCl priming on salt tolerance in melon seedling grown under saline conditions. Sciatica Horticulture, 97: 229-237. (Journal)
Soltani, A., Gholipoor, M. and Zeinali, E. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany, 55: 195-200. (Journal)
Voigt, E.L., Almeida, T.D., Chagas, R.M., Ponte, L.F.A., Viégas, R.A. and Silveira, J.A.G. 2009. Source–sink regulation of cotyledon reserve mobilization during cashew (Anacardium occidentale) seedling establishment under NaCl salinity. Journal of Plant Physiology, 166: 80–89. (Journal)
Wang, L., Chen, S., Kong, W., Li, S. and Archbold, D.D. 2006. Salicylic acid pretreatment alleviates chilling injury and affects the antioxidant system and heat shock proteins of peaches during cold storage. Postharvest Biology and Technology, 41: 244-251. (Journal)
Zehtabsalmasi, S. 2008. The effect of salinity on seed germination and pre-treatment of German chamomile. Research and Agriculture, 2(2): 28-30. (In Persian)(Journal)
Zhang, Y., Chen, K., Zhang, S.H. and Ferguson, I. 2003. The role of salicylic acid in postharvest ripening of kiwi fruit. Postharvest Biology and Technology, 28: 67-74. (Journal)