Effect of aging on enzymatic and non-enzymatic antioxidant changes and biochemical characteristics in barley (Hordeum vulgare L.) seeds cv. Valfajr

Document Type : Research Paper



This study was laid out to study the effect of accelerate aging duration onenzymatic and non-enzymatic antioxidant activity and biochemical characteristics of barley (Hordeum vulgare L.) seeds in Islamic Azad University, Boroujerd branch, at 2015 summer, that laid out in completely randomized design with four replications. Treatments included 5 accelerated ageing days (control and 1, 2, 3, 4 and 5 days). Results of analysis of variance showed that effect of ageing on germination percentage, electrical conductivity, soluble sugar, soluble protein,proline, acid ascorbic, catalase, peroxidase and ascorbat peroxidase enzymes were significant. Mean comparison results showed that, increase of ageing duration decreased germination percentage of barley seeds but, Malondialdeid, H2O2 content and electrical conductivity were increased. Soluble sugar and soluble protein were increased until 3 and 2 aging days respectively then decreased. With increasing of aging levels until two days proline content increased then decreased but, ascorbic acid production procedure was decreasing and ascorbic acid production in 1-4 ageing days was more than control. Ageing changed catalase, peroxidase and ascorbat peroxidase amount. With increasing of aging levels until 3 days catalase activity increased and until 2 days peroxidase and ascorbat peroxidase activity were increased then decreased. Lower ageing levels induced enzymatic and non-enzymatic damaging less than higher ageing levels but, with increasing of accelerated ageing until 5 days reactive oxygen species accumulation over come on these antioxidant systems. In this condition seed damage increased that laid to decrease of vigor and seed germination percentage.


Akhter, F.N., Kabir, G., Mannan, M.A. and Shaheen, N.N. 1992. Aging effect of wheat and barley seeds upon germination mitotic index and chromosomal damage. Journal of Islamic Academic of Science, 5: 44-48. (Journal)
Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Science Research, 14:93-107. (Journal)
Bailly, C., El-Maarouf-Bouteau, H. and Corbineau, F. 2008. From intracellular signaling networks to cell death: the dual role of reactive oxygen species in seed physiology. Comptes Rendus Biologies,331: 806–814. (Journal)
Bates, L.S., Walden, R.P. and Teave, I.D. 1973. Rapid defermination of free praline for water stress studies. Plant and Soil, 39:205-207. (Journal)
Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-252. (Journal)
Du, Z. and Bramlage, W.J. 1992. Modified thiobarbituric acid assay for measuring lipid oxidation in sugar-rich plant tissue extracts. Journal of Agricultural and Food Chemistry, 40: 1566-1570. (Journal)
Emam, Y. 2008. Cereal production. Shiraz University Press. 190 pp. (Book)
Fessel, S.A., Vieira, R.D., Cruz, M.C.P., Paula, R.C. and Panobianco, M. 2006. Electrical conductivity testing of corn seeds as influenced by temperature and period of storage. Pesqui Agropecu of Brasil, 41:1551–1559. (Journal)
Ghiazdowska, A., Krasuska, U. and Bogatek, R. 2010. Dormancy removal in apple embryos by nitric oxid or cyanide involves modifications in ethylene biosynthetic pathway. Planta, 232: 1397-1407. (Journal)
Gill, S.S. and Tuteja, N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48: 909-930. (Journal)
Goel, A. and Sheoran, I.S. 2003. Lipid peroxidation and peroxide-scavenging enzymes in cotton seeds under natural ageing. Biologia Plantarum, 46: 429–434. (Journal)
Goel, A., Coel, A.K. and Sheoran, I.S. 2003. Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. Journal of Plant Physiology, 160: 1093-1100. (Journal)
Govender, V., Aveling, T.A.S. and Kritzinger, Q. 2008. The effect of traditional storage methods on germination and vigor of maize (Zea mays L.) from northern KwaZulu-Natal and southern Mozambique. Southern African Journal of Botany, 74:190–196. (Journal)
Hampton, J.G. and Tekrony, D.M. 1995. Handbook of Vigor Test Methods. The International Seed Testing Association, Zurich 117 pp. (Handbook)
Heath, R.L. and Packer, L. 1968. Photoperoxidation in isolated chloroplast: kinetics and stoichiometry of fatty acid peroxidation. Biochemistry and Biophysics, 12: 189-198. (Journal)
Hu, D., Ma, G., Wang, Q., Yao, J.H., Wang, Y. and Pritchard, H. 2012. Spatial and temporal nature of reactive oxygen species production and programmed cell death in elm (Ulmus pumila L.) seeds during controlled deterioration. Plant Cell and Environment,35: 2045–2059. (Journal)
ISTA. 2012. International Rules for Seed Testing. Bassersdorf: International Seed Testing Association. (Handbook)
Kishor, P.B.K. and Dange, V. 1990. Sucrose metabolism in callus-cultures of cotton during growth. Indian Journal of Experimental Botany, 28: 352–355. (Journal)
Kong, L., Huo, H. and Moa, P. 2015. Antioxidant response and related gene expression in aged oat seed. Frontiers in Plant Science, 6: 1-9. (Journal)
Kong, Q., Mao, P.S., Yu, X.D. and Xia, F.S. 2014. Physiological changes in oat seeds aged at different moisture contents. Seed Science and Technology, 42: 190–201. (Journal)
Lehner, A., Mamadou, N., Poels, P., Come, D., Bailly, C. and Corbineau, F. 2008. Change in soluble carbohydrates, lipid peroxidation and antioxidant enzyme activityes in the embryo during aging in wheat grains. Journal of Cereal Science, 47(3): 555-565. (Journal)
Luck, H. 1962. Methods of enzymatic analysis. E.B. By Bergmeyer (1th edition), Verlag chemie weinheim Pp:885-894. (Book)
McCready, R.M., Guggolz, J., Silviera, V. and Owens, H.S. 1950. Determination of starch and amylase in vegetables. Analytical Chemistry, 22: 1156-1158. (Journal)
McDonald, M.B. 1999. Seed deterioration: physiology, repair, and assessment. Seed Science and Technology, 27: 177-237. (Journal)
Moller, I.M., Jensen, P.E. and Hansson, A. 2007. Oxidative modifications to cellular components in plants. Annual Review in Plant Biology,58: 459–481. (Journal)
Morrison, D.A., Auld, T.D., Rish, S., Porter, C. and McClay, K. 1992.Patterns of testa-imposed seed dormancy in native Australian legumes. Annals of Botany, 70: 157-163. (Journal)
Murthy, U.M.N., Kumar, P.P. and Sun, W.Q. 2003. Mechanisms of seed ageing under different storage conditions for Vigna radiate (L.)Wilczek: lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship to glass state transition. Journal of Experimental Botany, 54: 1057-1067. (Journal)
Narayana, U.M. and Wendell, Q.S. 2000. Protein modification by amadori and maillard reactions during seed storage: roles of sugar hydrolysis and lipid peroxidation. Journal of Experimental Botany, 348: 1221-1228. (Journal)
Newman, C.W. and Newman, R.K., 2006. A brief history of barley foods. Cereal Foods World, 51: 4–7. (Journal)
Nkang, A. and Umoh, E.O. 1997. Six month storability of five soybean cultivars as influenced by stage of harvest, storage temperature and relative humidity. Seed Science and Technology, 25: 93-99. (Journal)
Oliveira, J.T.A., Andrade, N.C., Martins-Miranda, A.S., Soares, A.A., Gondim, D.M.F. and Araujo, J.H. 2012. Differential expression of antioxidant enzymes and PR-proteins in compatible and incompatible interactions of cowpea (Vigna unguiculata) and the root-knot nematode Meloidogyne incognita. Plant Physiology and Biochemistry,51: 145–152. (Journal)
Priestley, D.A. 1986. Seed ageing. Cornell University Press, Ithava, New York. (Book)
Qaderi, M.M., Cavers, P.B. and Bernards, M.A. 2003. Pre- and post-dispersal factors regulate germination patterns and structural characteristics of Scotch thistle (Onopordum acanthium) cypselas. New Phytology, 159: 263–278. (Journal)
Resenda, M.L.V., Nojosa, G.B.A., Cavalcanti, L.S., Aguilar, M.A.G., Silva, L.H.C.P., Perez, J.O., Andrade, G.C.G., Carvalho, G.A. and Castro, R.M. 2002. Induction of resistance in coca against Crinipellis perniciosa and Verticillium dahlia by acibenzolar-s-methyl (ASM). Plant Pathology, 51: 624-628. (Journal)
Roozrokh, M. and Ghasemigolezani, K. 1999. Effect of seed ageing on emergence, yield and yield components in tow chickpea cultivars under complete and limited irrigation. M.Sc. thesis in Agronomy, Tabriz University, Tabriz, Iran. (In Persion)(Journal)
Soltani, A., Gholipoor, M. and Zeinali, E. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany, 55: 195-200. (Journal)
Spanò, C., Castiglione, M.R., Bottega, S. and Grilli, I. 2004. Natural ageing of wheat seeds. Current Topics in Plant Biology,5: 89–94. (Journal)
Sun, W.Q. and Leopold, A.C. 1994. Glassy state and seed storage stability: A viability equation analysis. Annals of Botany, 74: 601-604. (Journal)
Sun, W.Q. and Leopold, A.C. 1995. The Maillard reaction and oxidative stress during aging of soybean seeds. Plant Physiology, 94: 94–104. (Journal)
Sung, J.M. and Chiu, C.C. 1995. Lipid peroxidation and peroxide-scavenging enzymes of naturally aged soybean seed. Plant Science, 110: 45-52. (Journal)
Tavakolafshari, R., Ghasem, F., Majnoonhosseini, N., Alizadeh, H. and Bihamta, M.R. 2007. Effect of seed ageing on germination traits and catalase and peroxidase antioxidant activity in barley genotypes. Journal of Iranian Agricultural Science, 37(2): 337-346. (In Persion)(Journal)
Tina, X., Song, S. and Lei, Y. 2008. Cell death and reactive oxygen species metabolism during accelerated ageing of soybean axes. Russian Journal of Plant Physiology, 55: 33-40. (Journal)
Wojtyla, Ł., Garnczarska, M., Zalewski, T., Bednarski, W., Ratajczak, L. and Jurga, S. 2006. A comparative study of water distribution, free radical production and activation of antioxidative metabolism in germinating pea seeds. Journal of Plant Physiology,163: 1207–1220. (Journal)
Zamani, A., Sadatnoori, S.A. Tavakolafshari, R., Irannejad, H., Akbari. Gh.A. and Tavakoli, A. 2010. Atudy on lipid peroxidation and activity of some antioxidant enzymes in safflower seeds under accelerated and natural ageing. Iranian Journal of Crop Science, 41(3): 545-555. (In Persion)(Journal)
Zhang, M., Zhuo, J.J., Wang, X., Wu, S. and Wang, X. F. 2010. Optimizing seed water content: relevance to storage stability and molecular mobility. Journal of Integrative Plant Biology,52: 324–33. (Journal)