The evaluation of salt stress effect on antioxidant enzymes activity in seedling induced of Milk thistle primed seeds

Document Type : Research Paper

Authors

Abstract

This experiment was performed in order to evaluation antioxidant enzymes activity in seedling induced of milkthistle primed seeds in salt stress condition. The experiment was conducted in factorial based on completely randomized design with four factors and three replications in Agricultural Biotechnology Research Institute of Iran (ABRII). These factors were included of genotype (Amol and Majar), priming (primed and non-primed seeds), stress: salinity (150 mM) and control led (non-salinity) and time of sampling in 24 and 96 hours after sowing. The results showed the seed priming increased significantly the catalase (CAT) activity in Majar , proxidase(POX) activity in both genotypes and ascorbat peroxidase (APX) in Amol, so that CAT activity increased in Majar primed seeds than control 96%. Priming increased POX activity in Majar 14-fold greater than Amol. Increasing CAT and APX activity were observed in primed seeds in germination stage but POX activity increasing was observed in imbibition stage. Overall, the results showed the CAT, POX and APX activity was higher in primed seeds than non-primed in salt stress condition. Priming affected CAT and POX activity in Majar greater in Amol.

Keywords

References

Aebi, H. 1983. Methods of enzymatic analysis. 3rd ed. (Eds. Bergmeyer, H.U., J. Bergmeyer, M. Grabi 273-282, VCH Verlagsgesellschaft mbH, Germany. (Book)
Afzal, I., Aslam, N., Mahmood, F., Hameed, A., Irfan, S. and Ahmad, G. 2004.Enhancement of germination and emergence of canola seeds by different priming techniques. Caderno de Pesquisa Série Biologia Santa Cruz do Sul.16: 19-34. (Journal)
Amini, Z. 2007. Evaluation of antioxidant activity in barley under salt stress. MSc. Dissertation, International Emam-Khomeini University, Ghazvin, Iran. (In Persian)(Thesis)
Bradford, K.J., Steiner, J.J. and Trawatha, S.T. 1990. Seed priming influence and emergence of pepper seed lots. Crop Science, 30: 718-721. (Journal)
Cano, E.A., Bolaryn, M.C., Perez-Alfocea, F. and Caro, M. 1991. Effect of NaCl priming on increased salt tolerance in tomato. Journal of Horticulture Science, 66: 621-628. (Journal)
Corbineau, F. and Come, D. 2006. Priming: a technique for improving seed quality. International Seed Testing Association, 132: 38-40. (Handbook)
Copleland, L.O. and McDonald, M.B. 2001. Principles of seed science and technology. Kluwer Acadamic Publisher, USA. (Book)
Davork, Z., Kosina, P., Walterova, D., Simanek, V., Bachleda, P. and Ulrichova, J. 2003. Cell protection against model toxins by flavonolignans obtained from Silybum marianum. Toxicology Letters, 173: 201-212. (Journal)
Demir Kaya, M., Okcu, G., Atak, M., Cikili, Y. and Kolsarici, O. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annus L.). European Journal of Agronomy, 24: 291-295. (Journal)
Eisvand, H.R. 2008. Evaluation of seed priming effect on physiological quality of deteriorated tall wheat grass (Agropyron elongatum Host.) seeds under drought stress. Ph.D Dissertation, Tehran University, Karaj, Iran. (In Persian)(Thesis)
Hassanpour aghdam, M.B., Emarat Pardaz, J. and Farsad Akhtar, N. 2009. The effect of osmo-priming on germination and seedling growth of Brassica napus L. under salinity conditions. Journal of Food Agriculture and Environment, 7 (2): 620-622. (Journal)
Hsu, C.C., Chen, C.L., Chen, J.J. and Sung, J.M. 2003. Accelerated aging hence lipid per oxidation in bitter gourd seeds and effects of priming and hot water soaking treatments. Scientia Hortiulturae, 98: 201–212. (Journal)
Iqbal, M. and Ashraf, M. 2006. Wheat seed priming in relation to salt tolerance: growth, yield and level of free salicylic acid and polyamines. Annales Botanici Fennici, 43: 250-259. (Journal)
ISTA, 1996. International rules for seed testing. Seed Science and Technology, 13: 299-355. (Journal)
Kaur, S., Gupta, A.K. and Kaur, N. 2005. Seed priming increases crop yield possibly by modulating enzymes of sucrose metabolism in Chickpea. Journal of Agronomy and Crop Science, 191: 81-87. (Journal)
Khan, A.A. 1992. Preplant physiological seed conditioning. Horticulture Review, 13: 131-181. (Journal)
McDonald, M.B. 2000. Seed priming. In: Black, M., Bewley, J.D. (Eds.), Seed Technology and Its Biological Basis. Sheffield Academic Press, Sheffield, UK, pp. 287–325. (Book)
Miralli, M. 2009. Physiological and biochemical effects of abscisic acid on deteriorated canola (Brassica napus L.) seeds under low temperature stress. MSc. Dissertation, Tehran University, Karaj, Iran. (In Persian)(Thesis)
Moosavi, A. 2010. The effect of osmopriming on quantity and quality traits of Amaranthus forage and grain genotypes under salt and drought stress. MSc. Dissertation, Tehran University, Karaj, Iran. (In Persian)(Thesis)
Nakano, Y. and Asada, K. 1987. Purification of ascorbate peroxidase in spinach chloroplast: in ascorbate depleted medium and reactivation by monodehydro ascorbate radical. Plant Cell Phsiology, 28: 131-140. (Journal)
Rashid, A., Holligton, P.A., Harris, D. and Khan, P. 2006. On-farm seed priming for barley on normal, saline and saline-sodic soils. European Journal of Agronomy, 24: 276-281. (Journal)
Wang, H.Y., Chen, C.L. and Sung, J.M. 2003. Both warm water soaking and solid priming treatments enhance anti-oxidation of bitter gourd seeds germinated at sub-optimal temperature. Seed Science and Technology, 31: 47-56. (Journal)
Yagmur, M. and Kaydan, D. 2008. Alleviation of osmotic stress of water and salt stress in germination and seedling growth of triticale with seed priming treatments. African Journal of Biotechnology, 7(13): 2156-2162. (Journal)
Iranian Journal of Seed Sciences and Research
Volume 3, Issue 3
October 2016
Pages 11-21

Files

Share

How to cite

Statistics