The response of Arabidopsis mutants associated with senescence to the precursor of ethylene (ACC)

Document Type : Research Paper

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Abstract

Ethylene is involved in many developmental and processes including plant senescence. Senescence is the final step of plant development resulting in death of plants. 1-aminocyclopropane 1-carboxylic acid) ACC) is produced in the last step of the ethylene biosynthesis. The Morphological characteristics of treated plants by ACC induce a phenotype called triple response including root and hypocotyl shortness and thickness. The occurrence of these symptoms indicates that plant system sense ethylene and its biosynthesis is activate. In this project, we examined the response of the wild type seedlings Ler-0, Col-0 and several mutants including old-3, old-9, old-14, etr1-3 and ko-5 to various concentration of ACC in Arabidopsis thaliana following evaluation of triple response characteristics. OLD genes in Arabidopsis are known as senescence involved genes. According to the obtained results, it shown that with increasing ACC concentrations, the triple response has been detected in most mutants, while in mutant etr1-3 none of those responses were observed  meaning that etr1-3 is not able to sense and signal ethylene. These results suggest that change in OLD9, OLD3 and OLD14 function doesn't cause any significant impact on ethylene biosynthesis and signaling, while change in gene activity ETR in Arabidopsis led to the loss of the triple response in etr-3 plants. Thus, the genes controlling ethylene biosynthesis is different from genes regulating plant senescence.

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References

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Iranian Journal of Seed Sciences and Research
Volume 3, Issue 2
July 2016
Pages 67-80

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