Grouping of some canola genotypes in various drought stress treatment in Germination Stages based on multivariate statistical methods

Document Type : Research Paper

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Abstract

This study was conducted to grouping of 10 genotypes of canola via ISSR and IRAP markers and genotype resistance to drought. Eighty five polymorphic bands were produced using 19 individuals and combinations of ISSR and IRAP, and polymorphism varied between 50 to 100. Cluster analysis with UPGMA method placed the 10 genotypes in tree cluster, which includes 5, 3 and 2 respectively. Cluster analysis of germination results based on Euclidean distance with UPGMA method assigned treatment in four different groups consists of 11, 5, 9, and 15 genotypes respectively. Fisher’s linear Discriminant Function Analysis confirmed validity clustering analysis with 95%. The second group had the highest value, for most economical traits. SLM046 genotype could be introduced as resistant genotype by placing different treatments of its in one group. The fourth group included genotypes that had low value for most characteristics. Factor analysis results based on principal components showed that three factors named plumule, radicle and germination percentage, which determined 88.31 percent of total variation. First region was detected as good region on the base first and second factor evaluation. Genotypes in this area were favorable response to drought stress and could be introduced as resistant genotype at germination stage.

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References

Abdelmigid, H.M. 2014. Efficiency of random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers for genotype fingerprinting and genetic diversity studies in canola (Brassica napus L.). African Journal of Biotechnology, 11: 6409-6419. (Journal)
Akbari, G.A. and Abadi, M.R.L.H. 2007. Investigation of seed vigor and germination of canola cultivars under less irrigation in padding stage and after it. Pakistan Journal of Biological Sciences, 10: 2880-2884. (Journal)
Andalibi, B., Zangani, E. and Haghnazari, A. 2005. Effects of water stress on germination indices in six rapeseed cultivars (Brassica napus L.). Iranin Journal of Agricultural Sciences 36: 457-463. (In Persian)(Journal)
Bybordi, A. 2012. Effect of different ratios of nitrate and ammonium on photosynthesis and fatty acid composition of canola under saline conditions. International Journal of Agriculture and Crop Sciences, 4: 622-626. (Journal)
Chaves, M.M., Maroco, J.P. and Pereira, J.S. 2003. Understanding plant responses to drought from genes to the whole plant. Functional Plant Biology, 30: 239-264. (Journal)
Farshadfar, E., Zamani, M., Motallebi, M. and Imamjomeh, A., 2001. Selection for drought resistance in chickpea lines. Iranian Journal of Agricultural Sciences, 32: 65-77. (Journal)
Golabadi, M., Arzani, A. and Maibody, S.M. 2006. Assessment of drought tolerance in segregating populations in durum wheat. African Journal of Agricultural Research, 1: 162-171. (Journal)
Hosseini, F., Gharine, M.H., Bakhshande, A.A., Fathi, G.A. and Shirin, M. 2000. Study of Sodium seed Effects on germination and germination Components of five Canola genotype Brassica napus in vitro. Abstracts of the 1th National Conference on Science and Technology of Iran seed. University of Gorgan. (In Persian)
Huzsvai, L. and Rajkai, K. 2009. Modeling of plant adaptation to climatic drought induced water deficit. Biologia, 64: 536-540. (Journal)
Jajarmi, V. 2009. Effect of water stress on germination indices in seven wheat cultivar. World Academ. Sci. Eng. Technol, 49: 105-106. (In Persian)(Journal)
Johnson, B., Schneiter, A., McKay, K., Hanson, B. and Schatz, B. 1995. Influence of planting date on canola and crambe production. Journal of Production Agriculture, 8: 594-599. (Journal)
Kamkar, B., Ghaffari, H. and Entesari, M. 2008. The Study of Temperature and Salinity Effects on germination Components of Canola Cultivars, Journal of Agricultural Sciences and Natural Resources, 15: 1-12. (In Persian)(Journal)
Majidi, M.M. 2012. Identification of Canola Cultivars for Drought Tolerance in Germination and Seedling Growth Stages Using Principal Component Analysis. Journal of Crop Production and Processing, 2(4): 41-52. (In Persian)(Journal)
Naghavi, M.R., Khalili, M. and Aboughadareh, A.P. 2015. Effect of water deficit stress on yield and yield components of canola (Brassica napus L.) cultivars. International Journal of Agronomy and Agricultural Research, 7: 8-13. (Journal)
Nielsen, D.C. 1997. Water use and yield of canola under dryland. Journal of Production Agriculture, 10: 213. (Journal)
Safari, S., Mehrabi, A.A. and Safari, Z. 2013. Efficiency of RAPD and ISSR markers in assessment of genetic diversity in Brassica napus genotypes. International Journal of Agriculture and Crop Sciences, 5: 273-279. (Journal)
Saghai-Maroof, M.A., Soleiman, K., Jorgensen, R.A. and Allard, R.W. 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mandelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Sciences, 81: 8014-8018. (Journal)
Paulsen, G.M. 1987. Wheat stand establishment. Wheat and Wheat Improvement, 384-389. (Journal)
Iranian Journal of Seed Sciences and Research
Volume 3, Issue 2
July 2016
Pages 53-65

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