Assesment of maintenance of Ziziphus spina-christi (L.) Desf. seed under cryogenic conditions

Document Type : Research Paper

Authors

Abstract

Ziziphus spina-christi (L.) Desf. Is an arboreal species which grows in hot natuarl habitats of Southern Iran. Regarding the genetic diversity of the species in the country, collection and long-term preservation of seed germplasm in order to prevent genetic erosion and risk, have a great priority. To evaluate the possibility of long-term preservation of the Ziziphus spina-christi seeds under cryogenic conditions, three pre-cryopreservation methods including PVS2, Desiccation, and 30%Glycerol were applied before incubation of the seeds under cryopreservation conditions (-196°C) or lipuid nitrogen (LN). The treated seeds were incubated in LN for periods of 1 week, 1 month and 1 year. Subsequently, the cryopreserved seeds were removed from cryogenic condition and evaluated under laboratory and greenhouse conditions. The results showed that seeds of Ziziphus spina-christi are able to tolerate cryogenic conditions. Effects of the cryopreservation pre-treatments including 30%Glycerol, Desiccation and PVS2 were significantly different on seed attributes and, Desiccation showed the best effects on survival rates and other attributes of the cryopreserved seeds. The interaction effects between cryogenic storage periods and pretreatments were significant for some of the attributes. However some of the attributes did not show different means in different incubation periods. In greenhouse conditions, the cryopreserved seeds were germinated and established well and, Desiccation method showed better results compare to those of the 30%Glycerol and PVS2.

Keywords

References

Assareh, M.A. and Sabaghzadeh, F. 2002. Asexual proppagation of Christ's Thorn Jujube Ziziphus spina-christi (L.) Desf. by tissue culture.Pajohesh and Sazandegi,55: 19-22. (In Persian)(Journal)
Abdul-Baki, A.A. and Anderson, J.D. 1973. Vigour determination in soybean seed by multiple criteria. Crop Science, 13: 630-633.(Journal)
Chang, Y. and Reed, B.M. 1999. Extended cold acclimation and recovery medium alteration improve regrowth of Rubus shoot tips following cryopreservation. CryoLetters, 20(6): 371-376. (Journal)
Cho, E.G., Noor, N.M., Kim, H.H., Rao, V.R. and Engelmann, F. 2002. Cryopreservation of Citrus aurantifolia seeds and embryonic axes using a desication protocol. CryoLetters, 23(5): 309-316. (Journal)
De Carlo, A., Benelli, C. and Lambardi, M. 2000. Development of a shoot-tip vitrification protocol and comparison with encapsulation-based procedures for plum (Prunus domestica L.) cryopreservation. CryoLetters, 21: 215-222. (Journal)
Dinarvand, M. and Zarinkamar, F. 2006. Anatomy-taxonomy of the genus Ziziphus in Iran. Iranian Journal of Botany, 12(1): 36-41.(In Persian)(Journal)
Dixit, S., Mandal, B.B., Sangeeta, A. and Srivastava, P.S. 2003. Genetic stability assessment of plants regenerated from cryopreserved embryogenic tissues of Dioscorea bulbifera L. using RAPD, biochemical and morphological analysis. CryoLetters, 24 (2): 77-84. (Journal)
 Jebelli, M., Naderishahab, M.A. and Jafari, A.A. 2014. Cryopreservation of seeds of some of the forest species (Acacia tortilis (Forssk.) Hayne) and Acacia nilotica (L.) Delile. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 23 (1): 1103-111.(In Persian)(Journal)
Jebelli, M., Naderishahab, M.A., Feizi, H. and Jafari, A A. 2015. Cryopreservation of Populus Euphratica Oliv. seeds and establishment of the cryopreserved seeds under laboratory and green house conditions. Iranian Journal of Horticaltural, 46(2): 313-322. (In Persian)(Journal)
Jebelli, M., Naderishahab, M.A., Jafari, A.A. and Hatami, F. 2014 Seed cryopreservation of Robinia pseudoacacia L. Iranian Journal of Forest, 6(2): 245-254. (In Persian)(Journal)
Hao, Y., Cheng, Y. and Deng, X. 2003. Gus gene remains stable in transgenic citrus callus recovered from cryopreservation. CryoLetters, 24(6): 375-380. (In Persian)(Journal)
Kuleshova, L.L., MacFarlane, D.R., Trounson, A.O. and Shaw, J.M. 1999. Sugars exert a major influence on the vitrification properties of ethylene glycol–based solutions and have low toxicity to embryos and oocytes. Cryobiology, 38 (2): 119-130. (In Persian)(Journal)
Kushnarenko, S.V., Romadanova, N.V. and Reed, B.M. 2009. Cold acclimation improves regrowth of cryopreserved apple shoot tips. CryoLetters, 30(1): 47–54. (In Persian)(Journal)
Mandal, B.B. and Dixit-Sharma, S. 2007. Cryopreservation of in vitro shoot tips of dioscorea deltoidea Wall., an endangered medicinal plant: effect of cryogenic procedure and storage duration. CryoLetters, 28(6): 461–470. (In Persian)(Journal)
Matsumoto, T., Sakai, A. and Yamada, K. 1994. Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia japonica) by vitrification and subsequent high plant. Plant Cell Report, 13: 442-446. (Journal)
Mix-Wagner, G., Schumacher, H.M. and Cross, R.J. 2003. Recovery of potato apices after several years of storage in liquid nitrogen. CryoLetters, 24 (1): 33-42. (Journal)
Motamedi, H., Safary, A., Maleki, S. and Seyyednejad, S.M. 2009. Ziziphus spina-christi, a native plant from Khuzestan, Iran, as a potential source for discovery new antimicrobial agents. Asian Journal of Plant Sciences, 8(2): 187-190. (Journal)
Moustafa, A.E.A., El-Wahab, R.H.A., Helmy, M.A. and Batanouny, K.H. 1998. Phenology, germination and propagation of some wild trees and shrubs in south Sinai, Egypt. Egyptian Journal of Botany, 36: 91-107. (Journal)
Mozaffarian, V. 2004. Trees and shrubs of Iran. Farhang Moaser Publication, Tehran, Iran. (In Persian) (Book)
Ozden-Tokatli, Y., Ozudogru, E.A., Gumusel, F. and Lambardi, M. 2007. Cryopreservation of Pistacia spp. seeds by dehydration and one-step freezing. CryoLetters, 28(2): 83-94. (Journal)
Sabeti, H. 2004. Forests trees and shrubs of Iran. Yazd University Publication. pp:536. (In Persian) (Book)
Sakai A., Kobayashi, S. and Oiyama, I. 1991. Survival by vitrification of nucellar cells of navel orange (Citrus sinensis Osb.var. brasiliensis Tanaka) cooled to –196°C. Journal of Plant Physiology, 137: 465-470. (Journal)
Sanayaima, R.K., Kaur, A., Agrawal, A. and Babbar, S.B. 2006. Cryopreservation of in vitro–grown shoot tips of Crateva nurvala Buch. Ham, and important medicinal tree. CryoLetters, 27(6): 375–386. (Journal)
Sánchez, C., Martinez, M.T., Vidal, N., San–José, M.C., Valladares, S. and Vieitez, A.M. 2008. Preservation of Quercus robur germplasm by cryostorage of embryogenic cultures derived from mature trees and RAPD analysis of genetic stability. CryoLetters, 29 (6): 493–504. (Journal)
Sant, R., Taylor, M. and Tyagi, A. 2006. Cryopreservation of in vitro–grown shoot tips of tropical taro (Colocasia esculenta var. esculenta) by vitrification. CryoLetters, 27(3): 133–142. (Journal)
Schoenweiss, K., Meier–Dinkel, A. and Grotha, R. 2005. Comparison of cryopreservation techniques for long–term storage of ash (Fraxinus excelsior L.). CryoLetters, 26(3): 201–212. (Journal)
Senula, A., Keller, E.R.J., Sanduijav, T. and Yohannes, T. 2007. Cryopreservation of cold–acclimated mint (Mentha spp.) shoot tips using a simple vitrification protocol. Cryoletters, 28(1): 1–12. (Journal)
Seo, M.J., Shin, J.H. and Sohn, J.K. 2007. Cryopreservation of dormant herbaceous peony (Paeonia lactiflora Pall.) shoot–tips by desiccation. CryoLetters, 28(3): 207–213. (Journal)
Sherlock, G., Block, W. and Benson, E.E. 2005. Thermal analysis of the plant encapsulation–dehydration cryopreservation protocol using silica gel as the desiccant. CryoLetters, 26(1): 45–54. (Journal)
Stewart, P., Taylor, M. and Mycock, D. 2001. The sequence of the preparative procedures affects the success of cryostorage of cassava somatic embryos. CryoLetters, 22(1): 35-42. (Journal)
Towill, L.E., Bonnart, R. and Volk, G.M. 2006. Cryopreservation of Arabidopsis thaliana shoot tips. CryoLetters, 27(6): 352–360. (Journal)
Walters, C., Wheeler, L.J. and Stanwood, P.C. 2004. Longevity of cryogenically-stored seeds. Cryobiology, 48: 229-244. (Journal)
Zhao, Y., Wu, Y., Engelmann, F. and Zhou, M. 2001. Cryopreservation of axillary buds of grape (Vitis vinifera) in vitro plantlets. CryoLetters, 22(5): 321-328. (Journal)
Iranian Journal of Seed Sciences and Research
Volume 3, Issue 1
April 2016
Pages 111-120

Files

Share

How to cite

Statistics