Effect of bulb inoculation with four species mycorrhizal fungi on quantitative and qualitative yield of two lily species

Document Type : Research Paper

Authors

Abstract

Positive and beneficial effects of mycorrhizal symbiosis on growth of different plants have been demonstrated in the past. These fungi by creating a symbiotic relationship with the roots of plants leads to improved morphological and physiological characteristics and are balancing on soil biodiversity. Most of the crops are capable of establishing symbiosis with endo-mycorrhizal fungi, but so far, no study has been done on the possibility of establishing a symbiotic relationship between these fungi and lily. For this purpose, this study was performed to investigate the effect of different species of mycorrhizal fungi, including Glomus intraradices, Glomus etunicatum, Glomus mosseae and Glomus clorum on yield of Lily longiflourm and Lily ledebourii.This research carried outas a factorial in a Completely Randomized Design with three replications. The results showed that the root of L. longiflourm and L. ledebourii has developed a symbiosis at an acceptable level and the interaction of mycorrhiza on two species of lily is significant on investigated traits. The highest of root colonization percentage was observed in L. longiflourm inoculated with G. etunicatum, while, there was the lowest of root colonization percentage in L. ledebourii inoculated with G. clorum and L. ledebourii without inoculation. Also,the highest and lowest of plant height was observed in L. longiflourm inoculated with G. etunicatum and L. ledebourii without inoculation, respectively.Interaction effect oflily×mycorrhiza showedthe highest absorption of phosphorus, iron and zinc was observed in L. longiflourm and L. ledebourii inoculated with G. etunicatum. Actually,the results of this study indicate to affect symbiotic of G. etunicatum on growth and nutrients uptake that provides background to investigate the feasibility of its application to improve plant growth in lily.

Keywords


Al-Karaki, G.N. 2000. Growth of mycorrhizal tomato and mineral acquisition under salt stress. Mycorrhiza, 10: 51–54. (Journal)
Al-Karaki, G.N., Hammad, R. and Rusan, M. 2001. Response of two tomato cultivars differing in salt tolerance to inoculation with mycorrhizal fungi under salt stress. Mycorrhiza, 11: 43–47. (Journal)
Allen, M.F., Moore, T.S. and Christensen, M. 1980. Phytohormone changes in Bouteloua gracilis infected by vesicular-arbuscular mycorrhizae. I. Cytokinin increases in the host plant. Canadian Journal of Botany, 58: 371–374. (Journal)
Allen, M.F., Smith, W.K., Moore, T.S. and Christensen, M. 1981. Comparative water relations and photosynthesis of mycorrhizal and non-mycorrhizal Bouteloua gracilis H.B.K. New Phytologists, 88: 683–693. (Journal)
Asrar, A.A. and Elhindi, K.M. 2011. Alleviation of drought stress of marigold (Tagetes erecta) plants by using arbuscular mycorrhizal fungi. Saudi Journal of Biological Science, 19: 38–46. (Journal)
Azcón-Aguilar, C., Azcón, R. and Barea, J.M. 1979. Endomycorrhizal fungi and Rhizobium as biological fertilizers for Medicago sativa in normal cultivation. Nature, 279: 325–327. (Journal)
Bevege, D.I., Bowen, G.D. and Skinner, M.F. 1975. Comparative carbohydrate physiology of ecto and endomycorrhizas. In: Sanders, F.E., Mosse B. and Tinker, P.B. (Eds.), Endomycorrhizas (pp 149-175), Academic Press, New York. (Book)
Diop, T.A., Wade, T.K., Diallo, A., Diouf, M. and Gueye, M. 2003. Solanum cultivar responses to arbuscular mycorrhizal fungi: growth and mineral status. African Journal of Biotechnology, 2(11): 429-433. (Journal)
Dole, J.M. and Wilkins, H.F. 1999. Floriculture, principles and species. Prentice Hall, Inc. p 613. (Book)
Duponnois, R., Colombet, A., Hien, V. and Thioulouse, J. 2005. The mycorrhizal fungus Glomus intraradices and rock phosphate amendment influence plant growth and microbial activity in the rhizosphere of Acacia holosericea. Soil Biology and Biochemistry, 37: 1460-1468. (Journal)
Freitas, M.S.M., Martins, M.A. and Vieira, I.J.C. 2004. Produção e qualidade de óleos essenciais de Mentha arvensis em resposta a inoculação de fungos micorrízicos arbusculares. Pesq. Agropec. Bras. 39: 887-894. (Journal)
Gamalero, E., Trotta, A., Massa, N., Copetta, A., Martinotti, M.G. and Berta, G. 2004. Impact of two fluorescent pseudomonads and an arbuscular mycorrhizal fungus on tomato plant growth, root architecture and P acquisition. Mycorrhiza, 14: 185–192. (Journal)
Gupta, M.L., Prasad, A., Ram, M. and S. Kumar. 2002. 'Effect of the vesicular arbuscular mycorrhizal (VAM) fungus Glomus fasciculatum on the essential oil yield related characters and nutrient acquisition in the crops of different cultivars of menthol mint (Mentha arvensis) under field conditions. Bioresource Technology, 81(1): 77–79. (Journal)
Hanson, W.C. 1950. The photometric determination of phosphorus in fertilizers using the phosphor vanadomolybdate complex. Journal of Science in Food and Agriculture, 1: 172-173. (Journal)
Hirrel, M.C. and Gerdemann, J.W. 1980. Improved growth of onion and bell pepper in saline soils by two vesicular arbuscular mycorrhizal fungi. Soil Science and Society of American Journal, 44: 654-665. (Journal)
Kapoor, R., Giri, B. and Mukerji, K.G. 2002. Glomus macrocarpum: a potential bioinoculant to improve essential oil quality and concentration in dill (Anethum graveolens L.) and carum (Trachyspermum ammi (Linn.) Sprague). World Journal of Microbiology and Biotechnology, 18: 459-463. (Journal)
Kapoor, R., Giri, B. and Mukerji, K.G. 2004. Improved growth and essential oil yield and quality in Foeniculum vulgare mill on mycorrhizal inoculation supplemented with P-fertilizer. Bioresource Technology, 93: 3007-3011. (Journal)
Khaosaad, T., Vierheilig, H., Nell, M., ZitterlEglseer, K. and Novak, J. 2006. Arbuscular mycorrhiza alters the concentration of essential oils in oregano (Origanum sp., Lamiaceae). Mycorrhiza, 16(6): 443–446. (Journal)
Kormanik, P.P. and McGraw, A.C. 1982. Quantification of vesicular-arbuscular mycorrhizae in plant roots. In Methods and Principles of Mycorrhizal Research (N. C. Schenck, Ed.).  pp. 37-45. American Phytopathological Society, st Paul.
Liu, J., Maldonado-Mendoza, I., Lopez-Meyer, M., Cheung, F., Town, C.D. and Harrison, M.J. 2007. Arbuscular mycorrhizal symbiosis is accompanied by local and systemic alterations in gene expression and an increase in disease resistance in the shoots. Plant Journal, 50(3): 529-44. (Journal)
Maesato, K., Sharada, K., Fukui, H., Hara, T. and Sarma, S. 1994. In viro bulblet regeneration from bulb scale explants of Lilium japonicum Thunb. Effect of plant growth regulators and culture environment. Journal of Horticulture Society. 69(2): 289-297. (Journal)
Marschner, H. and Dell, B. 1994. Nutrient uptake in mycorrhizal symbiosis. Plant and Soil, 159: 89-102. (Journal)
Podila, G.K. and Douds, D.D. 2001. Current Advances in Mycorrhizae Research. APS Press, St. Paul. (Book)
Scheilenbaum, L., Sprenger, N., Schuepp, H., Wiemken, A. and Boller, E. 1999. Effect of drought transgenic expression of a fructan synthesizing enzyme and of mycorrhizal symbiosis on growth and soluble carbohydrate pools in tobacco plants. New Phytologists, 142: 67–77. (Journal)
Smith, S.E. and Read, D. 2008. Mycorrhizas in agriculture, horticulture and forestry. Mycorrhizal Symbiosis (Third Edition). Pp. 611-636.
Swift, C.E. 2004. Mycorrhiza and soil phosphorus levels(pp. 1-4). Colorado State University, Cooperation Extention. Retrieved December 19, 2005, from http://www.colostate.edu/Depts/CoopExt/TRA/PLANTS/mycorhiza.html?
Turjaman, M., Tamai, Y., Santoso, E., Osaki, M. and Tawaraya, K. 2006. Arbuscular mycorrhizal
fungi increased early growth of two nontimber forest product species Dyera polyphylla and Aquilaria filaria under greenhouse conditions. Mycorrhiza, 16: 459–464. (Journal)
Venkateshwar Rao, G., Manoharachary, C. and Rajeswara Rao, B.R. 2002. Beneficial influence of arbuscular mycorrhizal fungal association on growth, yield and nutrient uptake of rose-scented geranium (Pelargonium species). Philippine Journal of Science, 131: 49-58. (Journal)