University of Guilan Iranian Journal of Seed Sciences and Research 2476-3780 12 1 2025 06 21 The effect of vitamin U priming on physiological and biochemical characteristics of French bean seedlings (Phaseolus vulgaris cv. Sadri) under salinity stress The effect of vitamin U priming on physiological and biochemical characteristics of French bean seedlings (Phaseolus vulgaris cv. Sadri) under salinity stress 65 76 8929 10.22124/jms.2025.8929 FA Leyla Sedighi MSc student of Seed Science and Technology, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran Mohammad Sedghi Professor, Department of Production Engineering and Plant Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran Raouf Seyed Sharifi Professor, Department of Production Engineering and Plant Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran Hamideh Alidoost Ph.D of Crop Ecology, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran Journal Article 2025 04 17 In order to investigate the effect of vitamin U priming on physiological and biochemical characteristics of French bean seedlings under salinity stress and an experiment was conducted based on completely randomized design arranged in factorial with three replications at University of Mohaghegh Ardabili Laboratory in 2024. Treatments were four salinity levels (0, 50, 100 and 150 mM Derived from NaCl) and three levels of Vitamin U (0, 2 and 4 mM). The results showed that salinity stress reduced Germination rate, Germination percentage, Radicle length, Plumule length, radicle fresh and dry weight and plumule fresh and dry weight. But priming with distilled water, different levels of Vitamin U, especially the 4 mM level, improved these traits. The total protein content in the treatment with Vitamin U 4 mM compared to the control (distilled water) showed an increase of about 25. The amount of sodium and sodium / protein in priming with Vitamin U 4mM and without salinity compared to the the control (distilled water) and 150 mM salinity showed a decrease of about 84 and 97%, respectively. Also, the highest the amount of potassium was observed in the treatment with vitamin U 4 mM and without salinity. The results showed that seed treatment with different levels of vitamin U can reduce the harmful effects of salinity on some traits french bean seedlings and improve seedling growth. In order to investigate the effect of vitamin U priming on physiological and biochemical characteristics of French bean seedlings under salinity stress and an experiment was conducted based on completely randomized design arranged in factorial with three replications at University of Mohaghegh Ardabili Laboratory in 2024. Treatments were four salinity levels (0, 50, 100 and 150 mM Derived from NaCl) and three levels of Vitamin U (0, 2 and 4 mM). The results showed that salinity stress reduced Germination rate, Germination percentage, Radicle length, Plumule length, radicle fresh and dry weight and plumule fresh and dry weight. But priming with distilled water, different levels of Vitamin U, especially the 4 mM level, improved these traits. The total protein content in the treatment with Vitamin U 4 mM compared to the control (distilled water) showed an increase of about 25. The amount of sodium and sodium / protein in priming with Vitamin U 4mM and without salinity compared to the the control (distilled water) and 150 mM salinity showed a decrease of about 84 and 97%, respectively. Also, the highest the amount of potassium was observed in the treatment with vitamin U 4 mM and without salinity. The results showed that seed treatment with different levels of vitamin U can reduce the harmful effects of salinity on some traits french bean seedlings and improve seedling growth. https://jms.guilan.ac.ir/article_8929_e5bf463178abfcb53bb1b1e7716b3602.pdf