University of Guilan Iranian Journal of Seed Sciences and Research 2476-3780 8 4 2021 12 22 The inhibitory effect of Walnut and Elderberry hydro-alcoholic extract on germination, morphological and biochemical characteristics of Portulaca oleracea The inhibitory effect of Walnut and Elderberry hydro-alcoholic extract on germination, morphological and biochemical characteristics of Portulaca oleracea 384 371 5286 10.22124/jms.2021.5286 FA Mojtaba Salahiostad MSc Student, Department of Gardening, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Bahram Abedi Assistant Professor, Department of Gardening, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. Malihe Morshadloo MSc Student, Department of Gardening, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Maryam Ahangarani MSc Student, Department of Gardening, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Shoayb Jabbari Ghale Khaki MSc Student, Department of Gardening, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Zinab Asghary Dashtaby . MSc Student, Department of Gardening, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Ehsan Esmaeili MSc Student, Department of Gardening, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Journal Article 2019 07 09 In order to study germination characteristics and early growth of Portulaca oleracea, under different treatment of Walnut (W) and Elderberry (E), Two separate experiments were carried out in a factorial arrangement a Completely randomized with three replications over a farm season (1396). Treatments included Walnut and Elderberry hydro-alcoholic extract in concentrations of 0, 20, 60 and 100%. Results of the analysis of the variance of the laboratory and the greenhouse showed that the Walnut and Elderberry hydro-alcoholic extract had a significant effect on all the characteristics, So that the highest inhibitory effect was observed in 100% Walnut and 100% Elderberry. comparison of the average data from the experimental section showed that with increasing the the Walnut and Elderberry hydro-alcoholic extract in the experimental section germination percentage, germination speed, root length, stem length, fresh and dry weights, and indices of Portulaca oleracea seeds vigor index were reduced compared to witnesses. comparison of the average data from the greenhouse segment showed that with increasing the concentration of the extract stem length, Stem dry weight, Root dry weight, Number of leaves plant, Number of roots in the plant, photosynthesis pigments such as chlorophyll a, b and carotenoids increased significantly and proline increased. Also, the growth of the roots of the plant has a significant decrease in its meaning. which resulted in a decrease in the dry weight and dry weight of the Portulaca oleracea. according to the results obtained in this study Walnut and Elderberry hydro-alcoholic extract at a concentration of 100% concentration can be used to control Portulaca oleracea In order to study germination characteristics and early growth of Portulaca oleracea, under different treatment of Walnut (W) and Elderberry (E), Two separate experiments were carried out in a factorial arrangement a Completely randomized with three replications over a farm season (1396). Treatments included Walnut and Elderberry hydro-alcoholic extract in concentrations of 0, 20, 60 and 100%. Results of the analysis of the variance of the laboratory and the greenhouse showed that the Walnut and Elderberry hydro-alcoholic extract had a significant effect on all the characteristics, So that the highest inhibitory effect was observed in 100% Walnut and 100% Elderberry. comparison of the average data from the experimental section showed that with increasing the the Walnut and Elderberry hydro-alcoholic extract in the experimental section germination percentage, germination speed, root length, stem length, fresh and dry weights, and indices of Portulaca oleracea seeds vigor index were reduced compared to witnesses. comparison of the average data from the greenhouse segment showed that with increasing the concentration of the extract stem length, Stem dry weight, Root dry weight, Number of leaves plant, Number of roots in the plant, photosynthesis pigments such as chlorophyll a, b and carotenoids increased significantly and proline increased. Also, the growth of the roots of the plant has a significant decrease in its meaning. which resulted in a decrease in the dry weight and dry weight of the Portulaca oleracea. according to the results obtained in this study Walnut and Elderberry hydro-alcoholic extract at a concentration of 100% concentration can be used to control Portulaca oleracea

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