University of Guilan Iranian Journal of Seed Sciences and Research 2476-3780 8 3 2021 09 23 Effect of priming on seed germination and morpho-physiological traits of Portulacaoleracea L. under salinity stress Effect of priming on seed germination and morpho-physiological traits of Portulacaoleracea L. under salinity stress 293 310 5281 10.22124/jms.2021.5281 FA Zahra Hasani Master, Faculty of Agricultural Sciences, Shahed University, Tehran, Iran Nasim Amraie Master, Faculty of Agricultural Sciences, Shahed University, Tehran, Iran Khadijeh Ahmadi PhD student in Plant Physiology, Faculty of Agricultural Sciences, Shahed University, Tehran, Iran Heshmat Omidi Associate Professor and member of the faculty, Department of Agriculture and Plant Breeding, Faculty of Agricultural Sciences, Shahed University, Tehran, Iran Journal Article 2021 01 26 Salt tolerance at the germination stage is important, because salinity is usually severeat the soil surface.Especially in cases where the groundwater level is high.In order to investigate the effect of primingand salinity stress on the quantitative and qualitative traits of <em>P. oleracea</em>, this experiment was conducted in the Seed Technology Laboratory of the Faculty of Agricultural Sciences of Shahed University in 2019.Experimental agents included pretreatments (control (distilled water), hydro-priming, potassium nitrate and salicylic acid) in response to 6 salinity levels (no salinity as control and salinity of 30, 60, 90, 120 and 200 mM with Sodium Chloride).This experiment was studied as a factorial in a completely randomized design with three replications. The measured traits included germination indices, photosynthetic pigments, anthocyanin content, proline and soluble sugars.The results showed that the highest amount of germination components such as germination percentage (97%), germination rate (2.16 seeds per day), plantletlength (6.69 cm), plantletfresh weight (4 g), plantletlength index (324.69) and RWC (75.25%) were obtained in non-salinity stress and application of hydro-priming pretreatment.At salinity levels of 60 mM, the content of chlorophyll a, chlorophyll b and total chlorophyll in the hydropriming treatment increased and the highest content of proline, soluble sugars and anthocyanin was obtained at the highest salinity level.Among the studied priming levels, hydropriming at salinity stress of 200 mM had the highest percentage and seed germination rate, chlorophyll content, anthocyanin and proline. Salt tolerance at the germination stage is important, because salinity is usually severeat the soil surface.Especially in cases where the groundwater level is high.In order to investigate the effect of primingand salinity stress on the quantitative and qualitative traits of <em>P. oleracea</em>, this experiment was conducted in the Seed Technology Laboratory of the Faculty of Agricultural Sciences of Shahed University in 2019.Experimental agents included pretreatments (control (distilled water), hydro-priming, potassium nitrate and salicylic acid) in response to 6 salinity levels (no salinity as control and salinity of 30, 60, 90, 120 and 200 mM with Sodium Chloride).This experiment was studied as a factorial in a completely randomized design with three replications. The measured traits included germination indices, photosynthetic pigments, anthocyanin content, proline and soluble sugars.The results showed that the highest amount of germination components such as germination percentage (97%), germination rate (2.16 seeds per day), plantletlength (6.69 cm), plantletfresh weight (4 g), plantletlength index (324.69) and RWC (75.25%) were obtained in non-salinity stress and application of hydro-priming pretreatment.At salinity levels of 60 mM, the content of chlorophyll a, chlorophyll b and total chlorophyll in the hydropriming treatment increased and the highest content of proline, soluble sugars and anthocyanin was obtained at the highest salinity level.Among the studied priming levels, hydropriming at salinity stress of 200 mM had the highest percentage and seed germination rate, chlorophyll content, anthocyanin and proline. https://jms.guilan.ac.ir/article_5281_7e1b1578c7291518110a78c1611f68fc.pdf