University of Guilan Iranian Journal of Seed Sciences and Research 2476-3780 7 1 2020 05 21 Detection of Xanthomonas campestris pv. phaseoli and use of optimized method in bean seed lots health monitoring Detection of Xanthomonas campestris pv. phaseoli and use of optimized method in bean seed lots health monitoring 69 77 4318 10.22124/jms.2020.4318 FA Kobra Moslemkhany Research Assistant Professor, Seed and Plant Certification and Registration Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran Leila Zare Researcher, Seed and Plant Certification and Registration Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran Nima Khaledi Ph.D of Plant Diseases, Ferdowsi University of Mashhad, Khorasan Razavi, Mashhad, Iran Leila Frahani Researcher, Seed and Plant Certification and Registration Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran Shahla Hashemi Fesharaki Researcher, Seed and Plant Certification and Registration Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran Farshid Hasani Research Assistant Professor, Seed and Plant Certification and Registration Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran Babak Darvishi Research Assistant Professor, Seed and Plant Certification and Registration Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran Journal Article 2018 08 18 Common bacterial blight, caused by the seed-borne bacteria Xanthomonas axonopodis pv. phaseol, is important seed borne disease in been culture area in the world. Using healthy and certified seed is the major key to success for control disease distribution so seed health testing is routinely carried out in the certification programs. In this study cultivation diagnostic tests on selective medium, PCR and pathogenicity on susceptible cultivar Khomain for detection of the bacteria X. axonopodis pv. phaseoli were optimized. These optimized methods were used for assessing the health of bean seed samples obtained from different seed lots. The results showed that the use of selective-medium in culturing bacteria prior to PCR test, greatly reduces differentiation error of similar non-target colonies from pathogenic ones. Also performing PCR on suspected colonies isolated from selected media compared to PCR of seed extract, due to the removal of plant inhibitors, it reduces the possibility of false feedback. The mentioned methods check the health of selected seed samples from standard seed farms successfully and the results indicated the authentication seed health and non-contaminated seed lots. Common bacterial blight, caused by the seed-borne bacteria Xanthomonas axonopodis pv. phaseol, is important seed borne disease in been culture area in the world. Using healthy and certified seed is the major key to success for control disease distribution so seed health testing is routinely carried out in the certification programs. In this study cultivation diagnostic tests on selective medium, PCR and pathogenicity on susceptible cultivar Khomain for detection of the bacteria X. axonopodis pv. phaseoli were optimized. These optimized methods were used for assessing the health of bean seed samples obtained from different seed lots. The results showed that the use of selective-medium in culturing bacteria prior to PCR test, greatly reduces differentiation error of similar non-target colonies from pathogenic ones. Also performing PCR on suspected colonies isolated from selected media compared to PCR of seed extract, due to the removal of plant inhibitors, it reduces the possibility of false feedback. The mentioned methods check the health of selected seed samples from standard seed farms successfully and the results indicated the authentication seed health and non-contaminated seed lots.

https://jms.guilan.ac.ir/article_4318_971f2208afc98acf076b3f08c56e2384.pdf