University of Guilan Iranian Journal of Seed Sciences and Research 2476-3780 5 4 2018 12 22 Effect of seed priming with spermidine on boosting germination indicators and activity of some antioxidant enzymes in drought-stressed soybean seedlings Effect of seed priming with spermidine on boosting germination indicators and activity of some antioxidant enzymes in drought-stressed soybean seedlings 59 70 2946 10.22124/jms.2018.2946 FA Masoumeh Heidargholinezhad Agriculture Department Manoochehr Gholipoor Hemmat Pirdashti Hamid Abbasdokht Agronomy and Plant Breeding, Agriculture Faculty, Shahrood University of Technology Journal Article 2017 12 24 In order to evaluate the effect of priming by spermidin polyamine on increasing drought stress at germination and seedling growth stages of soybean, cv. Caspian, a factorial experiment was conducted based on completely randomized design with four replications at Sari Agricultural Sciences and Natural Resources University in 2016. The treatments were three drought stress levels (0, -2 and -4 bar) and three spermidine levels (0, 1 and 2 mM). The results showed that application of spermidine at the rate of 1 mM improved germination properties including germination maximum, germination rate, germination uniformity, times to 10, 50 and 90% germination, radicle length, seedling weight and final germination under mild drought stress (-2 bar). High dose of spermidine could not improve the tolerance to intensive stress in evaluated traits. Application of 1 mM spermidine led to increased activity of antioxidant enzymes ascorbat peroxidase, peroxidase, catalase, and amount of soluble protein in mild drought stress conditions compared to control. It was found no interaction between spermidine and drought stress for protein concentration. Generally results indicated that the pretreatment of seeds with spermidine improved tolerance to moderate drought stress through boosting germination indices and increasing activity of antioxidant enzymes in soybean seedlings. In order to evaluate the effect of priming by spermidin polyamine on increasing drought stress at germination and seedling growth stages of soybean, cv. Caspian, a factorial experiment was conducted based on completely randomized design with four replications at Sari Agricultural Sciences and Natural Resources University in 2016. The treatments were three drought stress levels (0, -2 and -4 bar) and three spermidine levels (0, 1 and 2 mM). The results showed that application of spermidine at the rate of 1 mM improved germination properties including germination maximum, germination rate, germination uniformity, times to 10, 50 and 90% germination, radicle length, seedling weight and final germination under mild drought stress (-2 bar). High dose of spermidine could not improve the tolerance to intensive stress in evaluated traits. Application of 1 mM spermidine led to increased activity of antioxidant enzymes ascorbat peroxidase, peroxidase, catalase, and amount of soluble protein in mild drought stress conditions compared to control. It was found no interaction between spermidine and drought stress for protein concentration. Generally results indicated that the pretreatment of seeds with spermidine improved tolerance to moderate drought stress through boosting germination indices and increasing activity of antioxidant enzymes in soybean seedlings. https://jms.guilan.ac.ir/article_2946_4f52c468ed677f0c6a015881a59e83c8.pdf