University of Guilan Iranian Journal of Seed Sciences and Research 2476-3780 1 2 2014 09 23 Identification and infection percentage determination of soybean [Glycine max (L.) Merr.] by seed born fungi in Golestan province and its relationship with quality of seeds produced in this region Identification and infection percentage determination of soybean [Glycine max (L.) Merr.] by seed born fungi in Golestan province and its relationship with quality of seeds produced in this region 13 26 1323 FA Morteza Gorzin Farshid Ghaderifar Seyed Esmaeil Razavi Ebrahim Zeinali Journal Article 2015 10 23 In this study, soybean seeds (cv. Katool) were collected randomly from fields in the various parts of Golestan province, including Bandargaz, Kordkuy, Gorgan, AliAbad, Azadshahr and Kalaleh. To recognize the seed borne fungi, soybean seeds were plated on acidified potato dextrose agar acidified medium (pH=4.5). After identification of fungal pathogens, seed germination test were carried before and after accelerated aging to determine seed quality. A total of four genera of seed borne fungies were identified in different regions of this province. Incidence rate of These four pathogens that included Alternaria sp., Fusarium sp., Phomopsis sp., and Cercospora sp., were very different in distinctive regions of the province. The most and least of fungual infections in seeds of the province produced by Alternaria sp. (22%) and Cercospora sp. (6.07%), respectively. Total fungal infections in seeds of Kordkuy (71.33% of the total seeds) were more than the other regions. The least seed infection (7.33%) were observed in seeds of Kalaleh. With the exception of Alternaria sp., other fungies reduced seed germination and vigor in the some areas. The fungus Phomopsis sp. was much more important than the other fungies. As, in all areas that seed infection by Phomopsis sp. were high (Bandargaz, Kordkuy and Gorgan), a seed germination and vigor decreased linearly. Due to the high amount of fungal infections of soybean seeds in Golestan province, and negative impact of these pathogens on seed germination and vigor, Researchers and seed producers inform about the type and incidence of fungies in each of these areas, and efforts to find appropriate solutions and adapted to areas for control and reduce the negative effects of pathogens on seed quality characteristics, will be cause for increasing of health and efficiency of agricultural ecosystems in the province. In this study, soybean seeds (cv. Katool) were collected randomly from fields in the various parts of Golestan province, including Bandargaz, Kordkuy, Gorgan, AliAbad, Azadshahr and Kalaleh. To recognize the seed borne fungi, soybean seeds were plated on acidified potato dextrose agar acidified medium (pH=4.5). After identification of fungal pathogens, seed germination test were carried before and after accelerated aging to determine seed quality. A total of four genera of seed borne fungies were identified in different regions of this province. Incidence rate of These four pathogens that included Alternaria sp., Fusarium sp., Phomopsis sp., and Cercospora sp., were very different in distinctive regions of the province. The most and least of fungual infections in seeds of the province produced by Alternaria sp. (22%) and Cercospora sp. (6.07%), respectively. Total fungal infections in seeds of Kordkuy (71.33% of the total seeds) were more than the other regions. The least seed infection (7.33%) were observed in seeds of Kalaleh. With the exception of Alternaria sp., other fungies reduced seed germination and vigor in the some areas. The fungus Phomopsis sp. was much more important than the other fungies. As, in all areas that seed infection by Phomopsis sp. were high (Bandargaz, Kordkuy and Gorgan), a seed germination and vigor decreased linearly. Due to the high amount of fungal infections of soybean seeds in Golestan province, and negative impact of these pathogens on seed germination and vigor, Researchers and seed producers inform about the type and incidence of fungies in each of these areas, and efforts to find appropriate solutions and adapted to areas for control and reduce the negative effects of pathogens on seed quality characteristics, will be cause for increasing of health and efficiency of agricultural ecosystems in the province.

https://jms.guilan.ac.ir/article_1323_eda1bb11307deb1c9f67a8335e8bf845.pdf