بهبود جوانه‌زنی بذر، رشد و خصوصیات بیوشیمیایی گیاهچه‌های ذرت با کاربرد نانواکسید مس سنتز شده از گیاه اکالیپتوس (Eucalyptus camaldulensis)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای فیزیولوژی گیاهان زراعی، گروه مهندسی تولید و ژنتیک گیاهی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 دانشیار، گروه مهندسی تولید و ژنتیک گیاهی، دانشگاه محقق اردبیلی، اردبیل، ایران

10.22124/jms.2023.7608

چکیده

به­منظور بررسی تاثیر نانواکسید مس سنتز شده از گیاه اکالیپتوس بر جوانه‌زنی بذر، رشد و خصوصیات بیوشیمیایی گیاهچه‌های ذرت، آزمایشی به­صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در دانشگاه محقق اردبیلی در سال 1400 اجرا شد. فاکتور‌های آزمایشی شامل غلظت‌های مختلف نانواکسید مس در پنج سطح (شاهد (صفر)، 5/2، 5، 10 و 20 میلی­گرم در لیتر) و روش­های کاربرد در دو سطح (پیش تیمار کردن و افزودن به بستر کاشت) بودند. نتایج نشان داد که در هر دو روش کاربرد نانوذره (افزودن به بستر کاشت و پیش‌تیمار نمودن بذر)، کاربرد غلظت‌های مختلف نانواکسید مس موجب افزایش معنی‌دار درصد و سرعت جوانه‌زنی، متوسط جوانه‌زنی روزانه، شاخص همزمانی جوانه‌زنی، شاخص قدرت، طول و وزن خشک گیاهچه‌های ذرت و کاهش میانگین مدت جوانه‌زنی، زمان تا 50 درصد جوانه‌زنی بذرهای ذرت گردید. در بین روش‌های کاربرد نانوذره، پیش­تیمار نمودن بذرها با غلظت 5/2 میلی­گرم در لیتر نانواکسید مس و افزودن غلظت 5 میلی‌گرم در لیتر نانوذره به بستر کاشت بیش­ترین تاثیر را در بهبود درصد و سرعت جوانه‌زنی، شاخص همزمانی بذر، طول و وزن خشک گیاهچه و شاخص قدرت و کاهش میانگین مدت جوانه‌زنی بذر داشت. کاربرد غلظت‌های مختلف نانو‌اکسید مس در هر دو روش کاربرد موجب افزایش میزان فعالیت آنزیم‌های کاتالاز، پراکسیداز، پلی‌فنول‌اکسیداز و محتوی اسید‌آمینه پرولین نسبت به تیمار شاهد گردید. به‌طور کلی کاربرد غلظت 5/2 میلی­گرم در لیتر نانواکسید مس به‌روش پیش­تیمار بذر و افزودن غلظت 5 میلی‌گرم در لیتر نانوذره به بستر کاشت جهت بهبود جوانه‌زنی بذر، رشد و خصوصیات بیوشیمیایی گیاهچه‌های ذرت پیشنهاد می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Improvement of seed germination, growth and biochemical characteristics of corn seedlings via the application of copper oxide nanoparticles synthesized from eucalyptus (Eucalyptus camaldulensis)

نویسندگان [English]

  • Mehdi Afrouz 1
  • Parisa Sheikhzadeh 2
1 Ph.D student of Crop Physiology, Department of Plant Production and Genetic Engineering, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 Associate Professor, Department of Plant Production and Genetic Engineering, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

In order to evaluate the effect of copper oxide nanoparticles synthesized from eucalyptus plant on seed germination, growth and biochemical characteristics of corn seedlings, and an experiment was carried out in a randomized complete block design with three replications at the University of Mohaghegh Ardabili in 2021. Experimental factors included synthesized copper oxide nanoparticles (0, 2.5, 5, 10, and 20 mg L-1) and the application methods of copper oxide nanoparticles (seed priming and adding to the planting medium). The results showed that in both methods of nanoparticle application (adding to the planting medium and priming), with the use of different concentrations of copper oxide nanoparticles, there was a significant increase in the percentage and speed of germination, average daily germination, germination synchronization index, strength index, length and dry weight. Corn seedlings and the reduction of the average germination time, the time for the germination of corn seeds to 50%. Among the methods of nanoparticle application, pretreating seeds with a concentration of 2.5 mg L-1 of copper oxide nanoparticles and adding a concentration of 5 mg L-1 of nanoparticles to the planting medium has the greatest effect on improving the percentage and speed of germination, seed synchronization index, length and dry weight. Seedlings and vigor index and decreased average seed germination time. The use of different concentrations of copper oxide nanoparticles in both application methods increased the activity of catalase, peroxidase, polyphenol oxidase and amino acid proline content compared to the control treatment. In general, it is recommended to use a concentration of 2.5 mg L-1 of copper oxide nanoparticles as a seed priming method and add a concentration of 5 mg L-1 of nanoparticles to the planting medium to improve seed germination, growth and biochemical characteristics of corn seedlings.

کلیدواژه‌ها [English]

  • Antioxidant enzyme activity
  • Green Synthesis
  • Nanotechnology
  • Seed priming
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