تأثیر پیش‌تیمار زیستی قارچ‌های تریکودرما و آسپرژیلوس در افزایش آستانه تحمل جذب روی در گیاه گندم

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته دکتری،گروه زراعت، پژوهشکده ژنتیک و زیست‏فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 دانش‌آموخته دکتری، گروه زراعت، پژوهشکده ژنتیک و زیست‏فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری

3 استاد گروه زراعت، پژوهشکده ژنتیک و زیست‏فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری

4 دانشیار گروه گیاهپزشکی، دانشگاه علوم کشاورزی و منابع طبیعی ساری

5 دانشیار گروه خاکشناسی، دانشگاه علوم کشاورزی و منابع طبیعی ساری

6 استادیار گروه زراعت، پژوهشکده ژنتیک و زیست‏فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری

10.22124/jms.2022.6159

چکیده

به‌منظور بررسی تأثیر پیش‌تیمار زیستی بذر گندم با قارچ‌های تریکودرما و آسپرژیلوس همراه با سولفات روی بر بهبود شاخص‌های جوانه‌زنی و میزان آستانه تحمل گیاهچه‌ها به عنصر روی، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار انجام شد. تیمارهای آزمایشی شامل شش سطح عنصر روی (صفر، 6/0، 6، 60، 300 و 600 میلی‌گرم در لیتر) و چهار تیمار قارچی (عدم تلقیح و تلقیح قارچ‏های Trichoderma harzianum، Trichoderma longibrachiatum و Aspergillus niger) بود. نتایج نشان داد که تیمارهای قارچی سبب افزایش قابل‌ملاحظه در فعالیت آنزیم ‌آنتی­اکسیدانی سوپراکسیددیسموتاز ساقه‌چه شدند. از طرفی بر اساس نتایج به­دست آمده همزیستی گیاهچه‌های گندم با قارچ‌های تریکودرما و به ویژه قارچ آسپرژیلوس موجب کاهش میزان پراکسیداسیون لیپیدی غشاء در تمامی سطوح کاربردی عنصر روی گردید. همچنین بیش‌ترین محتوای پرولین در بالاترین سطح عنصر روی در گیاهچه‌های گندم تلقیح‌با قارچ T. harzianum (افزایش چهار برابری نسبت به شاهد) و در سایر سطوح عنصر روی در گیاهچه‌های تلقیح شده با قارچ آسپرژیلوس مشاهده شد. از طرفی، نتایج بیانگر رابطه منفی بین محتوای مالون‌دی‌آلدئید با فعالیت آنزیم سوپراکسیددیسموتاز و شاخص‌های رشدی گیاهچه گندم بود. در مجموع، نتایج بیانگر افزایش توان کودپذیری گیاهچه‌های گندم تا غلظت 60 میلی‌گرم در لیتر از عنصر و در نتیجه اثربخشی بهتر عنصر روی در گیاهان تلقیح­شده با قارچ‌های تریکودرما و آسپرژیلوس به­ویژه در تلقیح با قارچT. Longibrachiatum بود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Trichoderma and Aspergillus fungi biopriming on increasing tolerance threshold of zinc uptake in wheat

نویسندگان [English]

  • Maryam Jenabiyan 1
  • Fatemeh Taghavi Ghasem Kheili 2
  • Hemmatollah Pirdashti 3
  • Mohammad Ali Tajick Ghanbari 4
  • Seyed Mostafa Emadi 5
  • Yasser Yaghoubian 6
1 Ph.D., Department of Agronomy, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University
2 Ph.D., Department of Agronomy, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University
3 Professor, Department of Agronomy, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University
4 Associate Professor, Department of Plant Phytopathology, Sari Agricultural Sciences and Natural Resources University
5 Associate Professor, Department of Soil Sciences, Sari Agricultural Sciences and Natural Resources University
6 Assistant Professor, Department of Agronomy, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University
چکیده [English]

In order to investigate the effect of biopriming of wheat seeds with Trichoderma and Aspergillusalong with ZnSo4 on improving germination indices and the tolerance threshold of wheat to zinc a factorial experiment was done based on a completely randomized design with three replications. Experimental treatments included six levels of zinc (0, 0.6, 6, 60, 300 and 600 mg/L) and four fungal treatments (no inoculation, inoculation with Trichoderma harzianum, Trichoderma longibrachiatum and Aspergillus niger). Nonetheless, based on the results, the coexistence of wheat seedlings with Trichoderma fungi especially Aspergillus fungi reduced the lipid peroxidation of the membrane in all application levels of zinc. Also, based on the results, the highest proline content was observed in the highest level of zinc in inoculated wheat seedlings by T. harzianum (fourfold increase as compared to the uninoculated control) and in other levels of zinc in seedlings inoculated with A. niger. However, the results showed a negative relationship between malondialdehyde (MDA) content and superoxide dismutase (SOD) activity and growth indices of wheat seedlings. There was also a negative relationship between MDAecontent with both SOD activity and growth indices of wheat seedlings. Inoculation of wheat seedlings with fungal treatments with higher production of SOD may led to higher resistance and avoidance to higher concentrations of Zn. Overall, the results showed an increase in the tolerance threshold of wheat seedlings to a concentration of 60 mg/L and therefore, more effectiveness of this micronutrient in seeds inoculated with Trichoderma and Aspergillus especially inoculated with Trichoderma longibrachiatum.

کلیدواژه‌ها [English]

  • Antioxidant
  • Fungi
  • Germination index
  • Malondialdehyde
  • Wheat
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