تاثیر عصاره گیاهان دارویی بر برخی شاخص‌های رشد و بیوشیمیایی کنجد

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تولیدات گیاهی، دانشکده کشاورزی بردسیر، دانشگاه شهید باهنر کرمان، کرمان

چکیده

جهت بررسی اثر عصاره شش گیاهی دارویی مقاوم به خشکی بر برخی شاخص­های رشد و بیوشیمایی کنجد، آزمایشی به صورت فاکتوریل در پایه طرح کاملأ تصادفی و با سه تکرار در آزمایشگاه تحقیقاتی دانشکده کشاورزی بردسیر، دانشگاه باهنر کرمان، در سال 1396 اجرا شد. تیمارهای آزمایش شامل شش عصاره گیاهی (درمنه (Artemisia annua)، شیرین بیان (: Glycyrrhiza glabra)، ترنجبین (pseudoalhagi Alhagi)، انغوزه (Ferula assafoetida)، تاغ ( Haloxylon ammodendron) و آویشن (Thymes vulgaris)) و غلظت عصاره در هفت سطح (صفر، 100، 500، 1000، 5000، 10000 و 20000 پی پی ام) بود. نتایج نشان داد که شاخص­های رشد شامل: درصد و سرعت جوانه زنی، خشک گیاهچه و بنیه بذر و صفات بیوشیمیایی شامل فعالیت آنزیم­های کاتالاز (CAT) و اسکوربات پراکسیداز (APX) تحت تاثیر اثرات ساده و متقابل هر دو تیمار آزمایش قرار گرفتند. فعالیت آنزیم گایاکول پراکسیداز (GPX) تنها تحت تاثیر اثر غلظت عصاره قرار گرفت، با افزایش غلظت عصاره تا سطح 5000 پی پی ام افزایش یافت و در سطح 10000 پی پی ام کاهش یافت. درصد و سرعت سبز شدن، وزن خشک گیاهچه و فعالیت آنزیم­های CAT و APX در کنجد با افزایش غلظت عصاره شیرین بیان افزایش یافت. عصاره­های آویشن، درمنه و تاغ سبب کاهش پارامترهای رشد و فعالیت آنزیم CAT و افزایش فعالیت آنزیم APX شد. آنغوزه و ترنجبین سبب افزایش طول کل گیاهچه و بنیه بذر در کنجد شدند و فعالیت آنزیم APX را کاهش دادند. بنابراین به نظر می‎رسد عصاره شیرین بیان از طریق محرک­های رشد و افزایش فعالیت آنزیم­های آنتی­اکسیدان سبب افزایش رشد گیاهچه­های کنجد شده است.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of medicinal plant extracts on some growth parameters and biochemical indices of sesame (Sesamum indicum)

نویسندگان [English]

  • Nasibeh Pourghasemian
  • mohadeseh said
  • Nima Ilkhani
Department of Plant Productions, Agricultural Faculty of Bardsir, Shahid Bahonar University of Kerman, Iran
چکیده [English]

In order to study on the effect of six medicinal plants of resistant to drought on some growth and biochemical parameters of sesame (Sesamum indicum), an experiment was conducted in a factorial arrangement based on completely randomized design with three replications in laboratory of Agricultural Faculty of Bardsir, Shahid Bahonar University of Kerman during 2016-2017. The experimental treatments were plant extract in six levels (Sugebrush (Artemisia annua), Licorice (Glycyrrhiza glabra), Camel thorn (Alhagi pseudoalhagi), Stinking assa (Ferula ssafoetida), Black saxaul (Haloxylon ammodendron), Thyme (Thymes vulgaris)) and extract concentrations at 7 levels (0, 100, 500, 1000, 5000, 10000, and 20000 ppm). The results showed that growth parameters as percentage and speed of germination, dry weight of plantlets, vigor index and biochemical parameters (CAT (Catalaze) and APX (Ascorbate peroxidase) activity) were affected by main and interaction effects of extract and concentration (P≤0.01). The GPX (Gayacol peroxsidase) activity was influenced by the main effect of extract concentration (P≤0.01). GPX activity increased with increasing of extract concentration to 5000 ppm and decreased at concentration of 10000 ppm. The percentage and speed of germination, dry weight of plantlets and CAT and APX activity were increased with increasing of Licorice extract concentration. Thyme, Sugebrush and Black saxaul extract reduced growth parameters and CAT activity and increased the APX activity. Camel thorn and Stinking assa extract increased root length and vigor index of plantlets and decreased APX activity.

کلیدواژه‌ها [English]

  • Ascorbate peroxidase
  • Catalaze
  • Gayacol peroxidase
  • Germination Percentage, Germination rate
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