تأثیر پیش‌تیمار بذر با اسپرمیدین بر بهبود شاخص‌های جوانه‌زنی و فعالیت برخی آنزیم-های آنتی‌اکسیدان گیاهچه‌های سویاتحت تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 جهاد کشاورزی مازندران

2 عضو هیئت علمی دانشگاه صنعتی شاهرود

3 دانشگاه علوم کشاورزی و منابع طبیعی ساری - پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان

4 زراعت و اصلاح نباتات، دانشگاه صنعتی شاهرود

چکیده

به­منظور بررسی تاثیر پیش­تیمار پلی­آمین اسپرمیدین بر افزایش تحمل به تنش خشکی در مرحله جوانه­زنی و رشد گیاهچه سویا رقم کاسپین، پژوهشی به صورت فاکتوریل در قالب طرح کاملا تصادفی با چهار تکرار در سال 1395 انجام شد. تیمارهای آزمایش شامل سه سطح تنش خشکی (صفر، 2- و 4- بار) و سه سطح اسپرمیدین (صفر، 1 و 2 میلی مولار) بودند. نتایج آزمایش نشان داد استفاده از اسپرمیدین در غلظت یک میلی مولار باعث بهبود معنی­دار خصوصیات جوانه­زنی شامل حداکثر جوانه­زنی، سرعت جوانه­زنی، یکنواختی جوانه­زنی، زمان­های رسیدن به 10،50 و90 درصد جوانه­زنی، طول ریشه­چه، وزن گیاهچه، و جوانه­زنی نهایی در تنش خشکی متوسط (2- بار) شد. غلظت بالای اسپرمیدین نتوانست باعث بهبود تحمل به تنش شدید در صفات مورد بررسی گردد. استفاده از اسپرمیدین یک میلی­مولار، بیشتر شدن فعالیت آنزیم­های آنتی­اکسیدان آسکوربات پراکسیداز، پراکسیداز، کاتالاز و میزان پروتئین محلول را در شرایط تنش خشکی متوسط نسبت به شاهد را به دنبال داشت. اثر متقابل معنی­داری بین پلی­آمین و تنش خشکی از نظر غلظت پروتئین مشاهده نشد. به طور کلی نتایج نشان داد که پیش­تیمار بذر با اسپرمیدین باعث افزایش تحمل به تنش از طریق بهبود شاخص­های جوانه­زنی و افزایش فعالیت آنزیم­های آنتی­اکسیدان گیاهچه­های سویا گردید.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of seed priming with spermidine on boosting germination indicators and activity of some antioxidant enzymes in drought-stressed soybean seedlings

نویسندگان [English]

  • Masoumeh Heidargholinezhad 1
  • Manoochehr Gholipoor 2
  • Hemmat Pirdashti 3
  • Hamid Abbasdokht 4
1 Agriculture Department
4 Agronomy and Plant Breeding, Agriculture Faculty, Shahrood University of Technology
چکیده [English]

In order to evaluate the effect of priming by spermidin polyamine on increasing drought stress at germination and seedling growth stages of soybean, cv. Caspian, a factorial experiment was conducted based on completely randomized design with four replications at Sari Agricultural Sciences and Natural Resources University in 2016. The treatments were three drought stress levels (0, -2 and -4 bar) and three spermidine levels (0, 1 and 2 mM). The results showed that application of spermidine at the rate of 1 mM improved germination properties including germination maximum, germination rate, germination uniformity, times to 10, 50 and 90% germination, radicle length, seedling weight and final germination under mild drought stress (-2 bar). High dose of spermidine could not improve the tolerance to intensive stress in evaluated traits. Application of 1 mM spermidine led to increased activity of antioxidant enzymes ascorbat peroxidase, peroxidase, catalase, and amount of soluble protein in mild drought stress conditions compared to control. It was found no interaction between spermidine and drought stress for protein concentration. Generally results indicated that the pretreatment of seeds with spermidine improved tolerance to moderate drought stress through boosting germination indices and increasing activity of antioxidant enzymes in soybean seedlings.

کلیدواژه‌ها [English]

  • Antioxidant enzymes
  • Drought tolerance boosting
  • Drought
  • Germination indicators
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