تاثیر نیتریک اکسید بر جوانه‌زنی و فعالیت برخی آنزیم‌های آنتی‌اکسیدانی کنجد (Sesamun indicum) تحت تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری

2 عضو هیئت علمی دانشگاه صنعتی شاهرود

چکیده

این آزمایش با هدف بررسی تأثیر نیتریک اکسید از منبع سدیم نیتروپروساید بر ویژگی­های جوانه­زنی و فعالیت برخی آنزیم‌های آنتی اکسیدانی کنجد تحت تنش شوری به‌صورت فاکتوریل بر پایه طرح کاملاً تصادفی در سه تکرار در آزمایشگاه مرکز تحقیقات بذر کاشمر اجرا شد. تیمارها شامل پیش تیمار بذر با سدیم نیتروپروساید در شش سطح (بدون پیشتیمار، آب مقطر، 50، 100، 150 و 200 میکرومولار) و تنش شوری در سه سطح (صفر، 50 و 100 میلی‌مولار در لیتر) بود. نتایج نشان داد که تنش شوری موجب کاهش شاخص­های جوانه­زنی و افزایش فعالیت آنزیم های آنتی اکسیدانی کنجد شد. پیش تیمار بذر با سدیم نیتروپروساید موجب افزایش سرعت جوانه­زنی، طول و وزن ریشه چه و ساقه چه در شرایط تنش شد و عملکرد بذر را بهبود بخشید. همچنین فعالیت آنزیم های سوپراکسیددیسموتاز، گلوتاتیون ردوکتاز و آسکوربات پراکسیداز نیز تحت تیمارهای نیتریک اکسید افزایش یافت در حالی که فعالیت آنزیم کاتالاز و محتوای مالون دی آلدئید به عنوان شاخص تخریب غشاء کاهش یافت. به طور کلی غلظت مناسب و کارا برای کاهش اثرات شوری در گیاهچه کنجد در این آزمایش غلظت 150 میکرومولار سدیم نیتروپروسایدبود، همچنین به نظر می رسد غلظت‌های بالاتر از 150 میکرومولار سدیم نیتروپروساید، اثرات سمیت نیتریک اکسید را بر سلول القا کند.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of nitric oxide on seed germination and activities of some antioxidant enzymes in sesame under salt stress

نویسندگان [English]

  • Alireza Fathi 1
  • Mehdi Baradaran 2
  • MohammadReza Amerian 2
چکیده [English]

The present experiment was aimed to investigate the effect of nitric oxide derived from sodium nitroprusside on sesame germination and some antioxidant enzymes activity under salinity stress in factorial manner and completely randomized design with three replications in seed research laboratory of Research Station in Eastern Iran (Kashmar). Treatments included seed pretreatment with sodium nitroprusside in six levels (zero, distilled water, 50, 100, 150 and 200 mM) and salinity stress at three levels (0, 50 and 100 mM l). The results showed that salt stress decreased germination rate, shoot and root length and dry weight and also were increase the activity of antioxidant enzymes superoxide dismutase, ascorbate peroxidase and glutathione reductase in sesame. Pretreatment seeds with SNP were increased seed germination, root and shoot length and weight. The enzyme activity of superoxide dismutase, glutathione reductase and ascorbate peroxidase treated with nitric oxide were increased, while catalase activity and the content of malondialdehyde as the index fell membrane degradation were decreased. In general, suitable and efficient concentration to reduce the effects of salinity on plant seeds in this experiment was 150 mM, because in this concentration was found the highest germination rate and dry weight of shoot and root. It also seems to concentrations above 150 mM sodium nitroprusside, toxic effects on the cells induce nitric oxide.

کلیدواژه‌ها [English]

  • Ascorbate peroxidase
  • Germination
  • Sodium nitroprusside
  • Superoxide dismutase
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