تأثیر تنش‌های خشکی و شوری بر خصوصیات جوانه‌زنی بذر، رشد و محتوای پرولین گیاهچه مینا چمنی (Bellis perennis L. “Bellissima Mixture”)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم باغبانی، دانشکده کشاورزی دانشگاه بیرجند

2 استادیار گروه علوم باغبانی دانشکده کشاورزی دانشگاه بیرجند

چکیده

جوانه­زنی و سبز شدن بذر به شدت تحت تأثیر تنش­های محیطی از جمله شوری و خشکی قرار می­گیرد. به منظور بررسی اثر تنش‌های خشکی و شوری بر جوانه­زنی بذور گیاه مینا چمنی (Bellis perennis L. BellissimaMixture)، دو آزمایش مجزا در قالب طرح کاملاً تصادفی با سه تکرار انجام شد. تیمارهای آزمایش‌های خشکی و شوری شامل هفت سطح پتانسیل اسمزی صفر، 2-، 4-، 6-، 8-، 10- و 12- بار بود که به ترتیب با استفاده از محلول پلی اتیلن گلیکول و کلرید سدیم اعمال شد. نتایج نشان داد که با افزایش سطوح تنش خشکی و شوری، درصد جوانه‌زنی کاهش پیدا کرد بطوریکه در سطح خشکی و شوری 12- بار، درصد جوانه‌زنی به ترتیب به صفر و 50 درصد کاهش یافت. تنش‌های خشکی و شوری به ترتیب از سطوح 2- و 8- بار به بالا سرعت جوانه‌زنی را بطور معنی‌داری در مقایسه با بذور شاهد کاهش دادند. همچنین تنش‌های خشکی و شوری باعث کاهش معنی دار طول ریشه­چه و ساقه­چه و وزن خشک ریشه‌چه، ساقه‌چه و کل شدند. مقادیر طول ساقه‌چه با افزایش سطح تنش شوری به 10- و 12- بار، به ترتیب 42 و 67 درصد در مقایسه با بذور شاهد کاهش یافتند. محتوای پرولین تحت تأثیر تنش های خشکی و شوری افزایش یافت ولی مقادیر پرولین در تنش خشکی در مقایسه با تنش شوری بیشتر بود. نتایج حاصل از این تحقیق نشان داد که تحمل به شوری گیاه مینا چمنی در مرحله جوانه­زنی در مقایسه با تنش خشکی بیشتر است.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of salinity and draught stresses on seed germination, growth and proline content of common daisy (Bellis perennis L. “Bellissima Mixture”) seedling

نویسندگان [English]

  • Hasan Bayat 1
  • Mohammad Hosein Aminifard 2
چکیده [English]

Germination and emergence of seed is strongly influenced by environmental stresses such as salinity and drought. In order to study the germination response of common daisy (Bellis perennis L. Bellissima Mixture) seeds to salinity and drought stresses, two separate experiments were carried out based on completely randomized design with 3 replications. Drought and salinity treatments were seven osmotic potential levels (0, -2, -4, -6, -8, -10 and -12 bars) applied using polyethylene glycol (PEG) and sodium chloride (NaCl), respectively. The results showed with increasing levels of drought and salinity, germination percentage decreased and at potential level of -12 bar of drought and salinity, it reduced to zero and 50%, respectively. Drought and salinity stresses significantly decreased germination rate from levels of -2 and -8 bar to higher compared to control, respectively. Moreover, drought and salinity stresses significantly reduced radical length, plumule length and dry weight of radical, plumule and total. With increasing levels of salinity to -10 and -12 bars, plumule length values decreased by 42 and 67% compared to control, respectively. Proline content significantly increased by drought and salinity stresses, but the amount of proline under drought stress was higher than salinity. The results of this study showed that the salinity tolerance of common daisy in the germination stage was higher than drought stress.

کلیدواژه‌ها [English]

  • Germination percentage
  • germination rate
  • NaCl
  • polyethylene glycol
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