اثر پیری تسریع شده بر جوانه‌زنی، رشد گیاهچه و برخی ترکیبات آنتی اکسیــدانی ژنوتیـپ‌های جـو

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشگاه شاهد

چکیده

به منظور بررسی اثر پیری تسریع شده بر فعالیت ترکیبات آنتی اکسیدانی و شاخص­های جوانه­زنی جو آزمایشی به صورت فاکتوریل در قالب طرح کاملا" تصادفی روی هشت ژنوتیپ بومی جو به همراه دو رقم شاهد به­رخ و نصرت در سه تکرار مورد بررسی قرار گرفت. تیمارهای آزمایشی شامل پیر کردن بذر در دو سطح پیری و عدم پیری(شاهد) و عامل دوم شامل ده ژنوتیپ جو بود.درصد جوانه­زنی پس از پیری در بیشتر ژنوتیپ ها کاهش یافت اما این کاهش نسبت به بذرهای پیر نشده معنی دار نبود.پیری تسریع شده به طور معنی­داری سرعت جوانه­زنی، طول ساقه­چه، طول ریشه­چه،غلظت رنگیزه­های فتوسنتزی و فعالیت آنزیم کاتالاز را کاهش داد. اما میانگین مدت زمان جوانه زنیو محتوای کل فنلی ژنوتیپ­ها و دو رقم به­رخ و نصرتپس از پیری افزایش یافت. ژنوتیپ شماره سه بیشترین فعالیت آنزیم کاتالاز را پس از پیری داشت. افزایش فنل در ژنوتیپ­ها متفاوت بود به طوری که ژنوتیپ شماره هشت بیشترین محتوای فنل را قبل و بعد از پیری داشت همچنین این ژنوتیپ نسبت به سایر ژنوتیپ­ها کمتر تحت تاثیر پیری تسریع شده قرار گرفت.به طور کلیژنوتیپ­هایی که محتوای فنلی و فعالیت آنزیمیبالایی داشتند از بنیه وسرعت  جوانه­زنی  بالاییپس از پیری بذر برخوردار بودند.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of accelerated aging on germination, seedling growth and some antioxidant compounds in barley genotypes

نویسندگان [English]

  • Zahra Moradian
  • Saeideh Maleki Farahani
  • Alireza Rezazadeh
SHAHED UNIVERSITY
چکیده [English]

In order to evaluate the effect of accelerated aging on antioxidant compounds and germination indices of barley, an experiment was conducted as factorial in a completely randomized design on eight native barley genotypes and two barley varietyBehrokh and Nosratwith three replications. The first factor was seed aging at two levels including aging and non-aging as control and the second factor was ten barley genotypes that the varieties were as control. Germination percentage of the most genotypes decreased after aging although it has not significant difference with non-aged seed. The results showed that accelerated ageing decreased significantly germination rate, shoot length and root length, concentration of photosynthetic pigments and catalase (CAT) activity. However mean germination time and total phenol content of genotypes and Behrokh and Nosrat varieties increased significantly after seed aging. Genotype 3 had the highestenzyme CAT activity after ageing. Increment of phenol content was different among the genotypes and genotype 8 had the highest phenol content before and after aging rather than the other genotypes and it affected less than the other genotypes by accelerated ageing. Totally the results of thisstudyindicated that genotypes that initially had high phenolic content and enzymatic activity also had vigor and germination rate after seed aging. 

کلیدواژه‌ها [English]

  • Carotenoids
  • Chlrophyill a
  • Enzyme
  • Hordeum
  • Proteins
  • Total phenolic content
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