اثر پرایمینگ ماتریکس بر بهبود برخی ویژگی‌های جوانه‌زنی بذر سه رقم سویا [Glycine max (L.) Merrill] رشد یافته تحت شرایط آبیاری محدود

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه آزاد واحد تاکستان

2 موسسه تحقیقات ثبت و گواهی بذر و نهال

3 دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران

4 ﻣﺆسسه تحقیقات اصلاح و تهیه نهال و بذر کرج

چکیده

جهت ارزیابی اثر پرایمینگ ماتریکس بر برخی ویژگی­های جوانه­زنی بذر سه رقم تجاری سویا، تولید شده تحت اعمال تنش خشکی بر گیاه مادری، تحقیقی مزرعه­ای و آزمایشگاهی به­صورت فاکتوریل به­ترتیب در قالب طرح بلوک­های کامل تصادفی و کاملاً تصادفی با 4 تکرار در مزرعه مؤسسه تحقیقات اصلاح و تهیه نهال و بذر و آزمایشگاه تجزیه کیفی بذر مؤسسه تحقیقات ثبت و گواهی بذر و نهال در کرج انجام شد. بذرهای 3 رقم تجاری سویایL17، Hamilton و Williams ‌با آبیاری گیاه مادر پس از 50، 100 و 150 میلی‌متر تبخیر از تشتک تبخیر کلاس A تولید شدند. سپس بذرها با محلول به نسبت وزنی1 به 4 بسترهای جامد کمپوست استریل، ورمیکولیت، پرلیت و زئولیت در آب مقطر پرایمینگ ماتریکس شدند و تیمار شاهد، بذرهای پرایمینگ ماتریکس نشده بودند. با آزمون جوانه­­زنی استاندارد درصد جوانه­زنی نهایی، درصد گیاهچه­های عادی و غیرعادی، متوسط زمان جوانه­زنی، ضریب سرعت جوانه­زنی، متوسط جوانه­زنی روزانه و سرعت جوانه­زنی روزانه تعیین شدند. نتایج نشان داد، اعمال تنش خشکی بر گیاه مادری سبب کاهش درصد جوانه­زنی نهائی، گیاهچه­های عادی و شاخص­های سرعت جوانه­زنی و افزایش درصد گیاهچه­های غیرعادی و متوسط زمان جوانه­زنی گردید. بذرهای رقم  L17در شرایط تنش خشکی از درصد جوانه­زنی نهائی بیشتر و درصد گیاهچه­های عادی کمتری نسبت به سایر ارقام مورد بررسی برخوردار بودند. به­طورکلی پرایمینگ ماتریکس سبب بهبود صفات مرتبط با جوانه­زنی مورد بررسی بذرهای تولید شده تنش خشکی گردید و با افزایش شدت تنش خشکی این بهبود مشهودتر بود. بسترهای جامد ورمی­کولیت، زئولیت، کمپوست و پرلیت به­ترتیب از اثر بهبود دهنده بیشتری برخوردار بودند. بذرهای رقم Hamilton تولید شده در شرایط تنش و بدون تنش خشکی، پرایمینگ ماتریکس شده با بستر جامد ورمی­کولیت از بیشترین بهبود برخوردار بودند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of matrix priming on some germination traits improvement of three commercial soybeans [Glycine max (L.) Merril] cultivars seeds grew by limited irrigation conditions

نویسندگان [English]

  • Seyed Mostafa Vaseei Kashani 1
  • Aidin Hamidi 2
  • Hosein Heidari Sharifabad 3
  • Jahanfar Daneshian 4
چکیده [English]

In order to evaluate the effect of matrix priming on some germination traits improvement of three soybean commercial cultivars seeds produced under parent plant drought stress, a field and laboratory experiment conducted as a factorial respectively based on randomized complete block and completely randomized design by four replications in Seed and Plant Improvement Institute(SPII) and the seed analysis laboratory of Seeds and Plant Certification and Registration Institute at Karaj. Three soybean commercial cultivars, L17, Hamilton and Williams seeds produced under parent plant drought stress by irrigation after 50, 100, 150 mm evaporation from A class pan evaporation. Then, seeds by 1 to 4 weight ratio of sterilized compost, vermiculate, perlite and zeolite solid matrices in distilled water matrix primed and not matrix primed seeds were control. By standard germination test, final germination percent, normal and abnormal seedlings percent, mean germination time, coefficient of velocity of germination, mean daily germination and daily germination speed determined. Results revealed, drought stress imposed on parent plant caused final germination, normal seedlings percent and germination rate indices decreased and abnormal seedlings percent and mean germination time increased. Under drought stress conditions L17 cultivar seeds had more final germination percent and less normal seedlings percent as compared with other studied cultivars. Totally, matrix priming caused produced under drought stress conditions seeds related to germination studied traits improvement and by increase drought stress intensity this improvement was more obvious. Also, vermiculate, zeolit, compost and perlite solid matrices respectively had more improvement effect. Hamilton cultivar seed produced without stress and under drought stress conditions, matrix primed by vermiculate solid matrix had the most improvement.  

کلیدواژه‌ها [English]

  • Compost
  • Perlite
  • Seed quality
  • Vermiculate
  • Zeolite
Almansoori, M., Kinet, M. and Lutts, Y. 2001. Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum des). Plant and Soil, 231: 243-254. (Journal)
Anonymous, 2007. Vermiculite insulation and asbestos, Information for REALTORS®. The Canadian Real Estate Association (CREA). (Book)
Anonymus, 2012. Iran plant varieties national list(vol. 1, Crop plants). Ministry of Jihad-e-Agriculture, Agricultural Research Education and Extensions Organization (AREEO), Seed and Plant Certification and Registration Institute (SPCRI). (In Farsi)
Anonymous, 2013a. FAO statistical yearbook, world food and agriculture Food and Agriculture Organization of the United Nations. Rome, Italy.
Anonymous. 2013b. Handbook for seedling evaluation (3rd ed). International Seed Testing Association (ISTA), Zurich, Switzerland. (Book)
Anonymous, 2013c. Seed and plant certification and registration institute (SPCRI) 10 years (2003-2013) revenue report. Ministry of Jihad-e-Agriculture Agricultural Research Education and Extensions Organization (AREEO), Seed and Plant Certification and Registration Institute (SPCRI). (In Farsi)
Anonymous, 2014. International rules for seed testing. International Seed Testing Association (ISTA). Seed Science and Technology. 38, Supplement. (Book)
Anonymous, 2015. Agriculture statistics, first volume-horticultural and field crops, 2012-13 crop year. Information and Communication Technology Center, Ministry of Jihad-e-Agriculture. (In Farsi)
Bewley, J.D., Bradford, K.J., Hilhorst, W.M. and Nonogaki, H. 2013. Seeds, physiology of development, germination and dormancr, 3rd ed., Springer Science+Buisiness Media, LLC. (Book)
Bradford, K.J. and Bewley, J.D. 2003. Seeds: biology, technology, and role in agriculture, In: Plants, genes, and crop biotechnology, By: Chrispeels, M.J.and ‎ Sadava, D.E. (sds.). Jones and Bartlett Publishers, Canada. (Book)
Cobaer, E.R., Cinazio, S.R., Pantalone, V.R. and Rajcan, I. 2009. Soybean. In: Oil crops. By: Vollmann, J. and Rajcan, I. eds., pp: 57-90, Springer Science+Buisiness Media, LLC. (Book)
Copeland, L.O. and‎ McDonald, M.B. 2001. Principles of seed science and technology, 4th. ed., Springer Science+Buisiness Media, LLC. (Book)
Dabrowska, B., Suchorska Tropilo, K. and Capecka, E., 2001. Pre sowing conditioning of hot pepper (Capsicum annum L.) seeds and its results in a field growing. Part І. Effect on the vigor of seeds and seeding. Annals Warsaw Agriculture University (Landscape Architecture), 22:3-7. (Journal)
Dastorani, M.T. and Poormohammadi, S. 2012. Evaluation of the effects of climate change on temperature, precipitation and evapotranspiration in Iran. International Conference on Applied Life Sciences (ICALS2012) Turkey, September 10-12, 2012, pp: 73-79. (Conference)
Dhanda, S.S., Sethi, G.S. and Behel, R.K. 2004. Indices of drought tolerance in wheat geynotypes at early stages ofplant growth. Journal of Agronomy and Crop Science, 190: 6-12. (Journal)
Dorenbos, D.L., Mullen, R.E. and Shibles, R.M. 1989. Drought stress effects during seed fill on soybean seed germination and vigor. Crop Science, 29: 476-480. (Journal)
Farooq, M., Wahid, A., Basra, S.M.A. and Siddique, K.H.M. 2010. Improving crop resistance to abiotic stresses through seed invigoration. In: Handbook of plant and crop stress, 3th. ed., By: Pessarakli, M., pp: 1031-1050, CRC Press, Taylor and Francis Group, London. (Book)
George, R.A.T. 2009. Vegetables seed production 3rd ed. CAB International. (Book)
Grzesik M., Dawidowicz–Grzegorzewska A. and Górnik K. 2000a. Effects of matriconditioning with Micro-Cel E on Callistephus chinensis L. seeds germination, seedling emergence, stress tolerance and some catabolic events. Acta Horticulturae, 517: 121–129. (Journal)
Grzesik M., Szafirowska A. and Sokołowska A. 2000b. Cytological and physiological effects of matriconditioning on cucumber seeds germination. Acta Horticulturae, 517: 113–120. (Journal)
Hampton, J.G. and TeKrony, D.M. 2005. Handbook of vigour test methods. International Seed Testing Association (ISTA), Zurich, Switzerland. (Book)
Harper, J. 1980. Population biology of plants academic press. New York. 892 pp. (Book)
Harris, D., Pathan, A.K., Gothkar, P., Joshi, A., Chivasa, W. and Nyamudeza, N. 2001. On- farm seed priming: using participatory method to revive and refine key technology. Method l revives and refines key technology. Agricultural System, 69: 51-164. (Journal)
Hartz, T.K., and Caprile, J. 1995. Germination of sh 2 sweet corn following seed disinfestations, solid- matrix priming and microbial seed treatment. Horticulture Science, 30:1400-1402. (Journal)
Kaya, M.D., Gamaze, O., Atal, M., Yakup, C. and Ozer, K. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24: 291-295. (Journal)
Kogel, J.E., Trived, N.G., Barker J.M. and Krakowsk, S.T. 2006. Industrial minerals and rocks, commodities, markets and uses. 7th ed., Society for Mining, Metallurgy and Exploration Inc. (SEM), Littleton, Colorado, USA. pp: 685-702. (Part of Book)
McDonald, M.B. 1999. Seed deterioration: Physiology, repair and assessment. Seed Science and Technology, 27: 177-237. (Journal)
McDonald, M.B. 2000. Seed priming. In "seed Technology and its Biological Basis." (M. Black and J.D. Bewley, Eds.), PP.287-325 Sheffield Academic press Ltd., Sheffield. (Part of Book)
McQuilken, M.P., Halmer, P. and Rhodes, D.J. 1998. Application of Microorganisms to Seeds. In: “Formulation of Microbial Biopesticides: Beneficial Microorganisms, Nematodes and Seed Treatments”. (Ed): Burges, H. D., Kulwer Academic Publishers, The Netherlands. pp: 255-285. (Part of Book)
Mercado, M.O.F. and Fernandez, P.G. 2004. Solid matrix priming of soybean seeds. Philippine Journal of Crop Science, 27(2): 27-35. (Journal)
Nouman, W., Basra, S.M.A., Siddiqui, M.T., Khan, R.A. and Mehmood, S. 2012. Seed priming improves the growth and nutritional quality of rangeland grasses. International Journal of. Agriculture and Biology, 14: 751–756. (Journal)
Olszewski, M.W., Goldsmith, R.S., Guthrie, E.K. and Young, C.A. 2012. Use of sieved compost plus hydrogel for solid matrix priming of carrot seeds. Compost Science and Utilization, 20(1): 5-10. (Journal)
Özdemir, E. and Sade, B. 2015. Alternative approach for drought tolerance; seed priming and physiology. International Conference on Chemical, Agricultural and Biological Sciences (CABS-2015) Sept. 4-5, 2015 Istanbul (Turkey), pp: 73-78. (Conference)
Parera, C.A., Qiao, P. and Cantliffe, D.J. 1993. Enhanced celery germination at stress temperature via solid matrix priming. Hortscience, 28(1):20-22. (Journal)
Pill, W.G. and Necker, A.D. 2001. The effects of seed treatments on germination and establishment of Kentucky blue grass (Poa pretense L.). Seed Science and Technology, 29: 65-72. (Journal)
Powell, A.A. 2007. Seed vigour and its assessment. In: Basra, A.S. (Ed.) Handbook of seed science and technology. Scientific Publishers, India. pp: 603-648. (Part of Book)
Ranal M.A. and De Santana D.G. 2006. How and why to measure the germination process? Revista Brasil. Botanicue. 29(1):1-11. (Journal)
Raziei, T., Bordi, I. and Pereira, L.S. 2008. A precipitation-based regionalization for Western Iran and regional drought variability. Hydrology and Earth System Science, 12: 1309–1321. (Journal)
ShiraniRad, A.H., Armand Pisheh, O. and Kazemian, H. 2014. Zeolites and their application in agriculture. Ministry of Jihade-e-Agriculture, Agricultural Research, Education and Extension Organization, Seed and Plant Improvement Institute (SPII). (In Farsi)
Smalley, M. 2006. Clay swelling and colloid stability. Taylor and Francis, CRC Press, Boca Raton, FL, USA. (Book)
Taylor, A.G., Allen P.S., Bennett, M.A., Bradford, K.G., Burris, G.S. and Misra, M.K., 1998. Seed enhancements. Seed Science Research, 8: 245-256. (Journal)
Taylor, A.G., Klein, D.E. and Whitlow, T.H. 1988. SMP: Solid matrix priming of seeds. Scientia Horticulturae, 37(1–2): 1–11. (Journal)
TeKrony, D.M. and Egli, D.B. 1993. Relationship of seed vigor to crop yield: a review. Crop Science, 31: 816-822. (Journal)
Ueda, A., Kathiresan, A., Inada, M. ,Narita, Y., Nakamura, T., Shi, W., Takabe, T. and Bennett, J. 2004. Osmatic stress in barely regulates expression of a different st of genes than salt stress dose. Journal of Experimental Batony, 55: 2213-2218. (Journal)
Vieira, R.D., TeKrony, D.M. and Egli, D.B. 1991. Effect of drought stress on soybean seed germination and vigor. Journal of Seed Technology, 16: 12-21. (Journal)
Wu, L., Hallgren, S.W., Ferris, D.M., and Conway, K.E. 1999. Solid matrix priming to enhance germination loblolly pine (Pinus taeda L.) seeds. Seed Science and Technology, 27: 251-261. (Journal)
Xiao, Z.W. and Jiarui, F. 1997. Priming effects of matriconitioning on Brassica. Para Chinese’s Loseeds. Act Scientiarum Naturalism Universities Sunyatseni, 36: 69-73. (Journal)