Effect of nano-zinc oxide and drought stress on the activity of hydrolytic enzymes and seed reserves mobilization of soybean (Glycine max. L) cultivar Katul (DPX)
Mohammad
Sedghi
Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran
author
Sahar
Tolouie
university of tabriz
author
text
article
2018
per
To evaluate the effect of drought stress originated from poly ethylene glycol and application of nano ZnO on the activity of hydrolytic enzymes and soybean seed reserves mobilization a factorial experiment was conducted based on completely randomized design with three replications at the University of Mohaghegh Ardabili in 2016. Treatments were drought stress at three levels as 0, -0.7 and -1.4 MPa and nano zinc oxide at the concentrations of 0, 0.75 and 1.5 g lit-1. Germination percentage and rate, seedling length and dry weight, activity of hydrolytic enzymes (alpha-amylase, protease and lipase) and seed reserves (carbohydrate, protein and lipid) mobilization were measured. Results showed that nano zinc oxide had the positive effect on germination percentage and rate, activity of hydrolytic enzymes and mobilization of carbohydrate, lipid and protein reserves. So that application of 1.5 g lit-1 of nano ZnO increased the activity of lipase, protease and α-amylase about 11.75, 12.75 and 11.87%, respectively in severe drought stress compared to non-stress condition. Drought stress also decreased the seed reserves mobilization in addition to reducing the activity of hydrolytic enzymes. There was a reduction trend in the mobilization of carbohydrates, lipids and proteins under drought stress with the increasing in imbibition time, while application of nano ZnO increased the mobilization of seed reserves which is the indicator of growth and production of higher seedling dry weight. Considering the ameliorative effect of nano ZnO on the damages due to drought stress and increasing the seed reserves using efficiency, activity of hydrolytic enzymes and germination percentage it seems that application of nano ZnO in the concentration of 1.5 g lit-1 will improve the soybean seed germination under drought conditions.
Iranian Journal of Seed Sciences and Research
University of Guilan
2476-3780
5
v.
3
no.
2018
1
17
https://jms.guilan.ac.ir/article_2931_835b85fb45e900bf26c1f35b66a89a64.pdf
dx.doi.org/10.22124/jms.2018.2931
Study of anatomical of seed coat infrastructural in some bulbous plants of Zanjan province
Zahra
Ghahramani
استادیار گروه علوم باغبانی دانشکده کشاورزی دانشگاه زنجان
author
Taher
Barzegar
استاد راهنما
author
Rasoul
Mozafari
فارغ التحصیل کارشناسی ارشد رشته باغبانی
author
Mahnaz
Vafadar
استادیار گروه زیست شناسی دانشگاه زنجان
author
text
article
2018
per
In order to identification and morphological evaluation of some bulbous plants of Zanjan province, four plant species with local names 'of Matar', 'Comran', 'Musir' and 'Iphlikcheh' was studied in three regions of Zanjan, Tarom and Dandy. The morphological results based on the flora of Iranica, Turkey, colored floraofIranand a taxonomic review of Allium Subg. Melanocrommyum in Iran indicated that 'Matar', 'Comran' and 'Sallot' with scientific names of Allium ubipetrence, Allium atroviolaceum Boiss and Allium jesdianum Boiss, respectively belong to Alliaceae family and 'Iphlikcheh' with scientiific name of Leopoldia comosa belong to Asparagaceae family. The microscopic studies on seed showed that the seeds shape of all species investigated were ovate to broadly ovate but the seed coat pattern was variable. The seed shape of L. comosa was morphologically quite different from the seed shape of other plants.
Iranian Journal of Seed Sciences and Research
University of Guilan
2476-3780
5
v.
3
no.
2018
19
31
https://jms.guilan.ac.ir/article_2932_79e4453b05c5e1d077b0aedcf2d1761d.pdf
dx.doi.org/10.22124/jms.2018.2932
The study of germination, biochemical and enzymatic characteristics of buckwheat (Fagopyrum esculentum Moench) affected by drought and salinity stresses
Mehdi
Aghighi Shahverdi
دانشگاه شاهد
author
arezoo
paravar
shahed university
author
Mojtaba
Ghasemzadeh
شیراز
author
Atefeh
Navabi
دانشگاه علوم پزشکی اراک
author
text
article
2018
per
In order to evaluate the effect of drought and salinity stresses on germination, biochemical and enzymatic characteristics of buckwheat seed were conducted two separate experiments in the laboratory of Seed Science and Technology of Shahed University in 2016. The experiments were conducted in a completely randomized design with three replications. Treatments were including eight levels of osmotic potential (zero, -2, -4, -6, -8, -10, -12and -14 bar) of drought and salinity. Analysis of variance showed were significant the effects of drought and salinity on percentage and rate of germination, seedling length, vigor, chlorophyll content, soluble protein, superoxide dismutase and catalase activity. The results of salinity and drought stresses on measured parameters showed that the effects of salinity and drought were inhibition, but inhibition of osmotic stress of salinity was highest from drought, as a result, no normal germination occurred in the salinity of 10 bar and in drought -14 bar. The decrease in chlorophyll and protein content under drought stress was more of salinity stress and conditions there led to increased activity of catalase under drought stress and superoxide dismutase under salinity stress. The results suggest that buckwheat drought compatibility and more resistant to salinity stress is applied.
Iranian Journal of Seed Sciences and Research
University of Guilan
2476-3780
5
v.
3
no.
2018
33
46
https://jms.guilan.ac.ir/article_2933_946bb604b2e805f4c567062ccb56396f.pdf
dx.doi.org/10.22124/jms.2018.2933
Effect of Nano Titanium Dioxide and Sodium nitroprusside on seed germination, vigor index and antioxidant enzymes of Afzal barley seedling under salinity stress
Afshin
Karami
دانشجوی دکتری، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه بوعلی سینا
author
Ali
Sepehri
عضو هیات علمی گروه زراعت و اصلاح نباتات دانشکده کشاورزی دانشگاه بوعلی سینا
author
text
article
2018
per
Salinity is the most important factor limiting growth and yield of crops in many area of the world. In order to study the effects of nano-TiO2 (0, 500, 1000, 2000 mg/L) and sodium nitroprusside (0 and 100 μM) on seed germination and antioxidant content of barley (var. Afzal) under salinity stress (0, 50 and 100 mM Sodium chloride) a factorial experiment based on a completely randomized design with four replications was conducted. Salinity stress declined the germination indices such as, germination percentage, germination rate, radicle length, plumule length, vigor index, mean germination time, relative water content and increased the membrane electrolyte leakage. In the presence of nano-TiO2, most of germination indices improved and the content of CAT, APX and especially SOD enzymes increased. Use of Sodium nitroprusside diminished the adverse effects of salinity stress and decreased the membrane electrolyte leakage. Also the interaction effect of sodium nitroprusside and nano-TiO2 was significant on the most studied traits. Nano-TiO2 (500 mg/L) with SNP improved SOD, APX and CAT enzyme contents by about 28, 16.8 and 17.9 % under 100 Mm salinity stress. According to the results, it seems that sodium nitroprusside diminished the adverse effects of salinity stress and impressed the nano-TiO2 performance on barley germination indices and antioxidant enzymes content.
Iranian Journal of Seed Sciences and Research
University of Guilan
2476-3780
5
v.
3
no.
2018
47
61
https://jms.guilan.ac.ir/article_2934_7066882deb139fc0e15263ae83177052.pdf
dx.doi.org/10.22124/jms.2018.2934
Nutritional assessment of micronutrients on quantitative and qualitative characteristics of amaranth seed production (Koniz)
Elnaz
Farajzadeh
استاد
author
text
article
2018
per
The aim of this study was to determine the best amount and timing of fertilizer micro zinc, iron and manganese on yield and seed quality is produced by Amaranth. Field experiments for combined analysis factorial randomized complete block design with three replications in two years (2014-2015) were conducted at the research station of Islamic Azad University of Malekan. The factors included micro fertilizers (iron, zinc and manganese), four different concentrations (7, 5, 3 per thousand and zero or non-use) and three stages (vegetative, reproductive and vegetative + reproductive + grain filling) was considered. The results showed that the highest number of seeds and grain yield (respectively 4460 number 17/99 gr/crop) were obtained using an iron concentration of 7 per thousand. Grain yield increase of 29/24 percent than control. The highest protein content was obtained by foliar application 7 per thousand micronutrients. Maximum panicle was 3/042 number by foliar application 7 per thousand on three phases. Maximum chlorophyll content (32/1 CCI) by application iron with 5 per thousand concentration. The maximum oil grain and oil yield, respectively (33/59%) and (6/083 gr/crop) if foliar application with zinc 7 per thousand concentrations were obtained. Based on the results of traits for fertilizer micro traits can be stated that maximum of the grain, grain yield, oil content and oil yield was achieved by foliar application 7 per thousand element and 7 per thousand micro elements concentration of protein content increased.
Iranian Journal of Seed Sciences and Research
University of Guilan
2476-3780
5
v.
3
no.
2018
63
75
https://jms.guilan.ac.ir/article_2935_06f8879f533e89f6906d346ae7b7f5cc.pdf
dx.doi.org/10.22124/jms.2018.2935
The effect of nitric oxide on seed germination and activities of some antioxidant enzymes in sesame under salt stress
Alireza
Fathi
دانشجوی دکتری
author
Mehdi
Baradaran
عضو هیئت علمی دانشگاه صنعتی شاهرود
author
MohammadReza
Amerian
عضو هیئت علمی دانشگاه صنعتی شاهرود
author
text
article
2018
per
The present experiment was aimed to investigate the effect of nitric oxide derived from sodium nitroprusside on sesame germination and some antioxidant enzymes activity under salinity stress in factorial manner and completely randomized design with three replications in seed research laboratory of Research Station in Eastern Iran (Kashmar). Treatments included seed pretreatment with sodium nitroprusside in six levels (zero, distilled water, 50, 100, 150 and 200 mM) and salinity stress at three levels (0, 50 and 100 mM l). The results showed that salt stress decreased germination rate, shoot and root length and dry weight and also were increase the activity of antioxidant enzymes superoxide dismutase, ascorbate peroxidase and glutathione reductase in sesame. Pretreatment seeds with SNP were increased seed germination, root and shoot length and weight. The enzyme activity of superoxide dismutase, glutathione reductase and ascorbate peroxidase treated with nitric oxide were increased, while catalase activity and the content of malondialdehyde as the index fell membrane degradation were decreased. In general, suitable and efficient concentration to reduce the effects of salinity on plant seeds in this experiment was 150 mM, because in this concentration was found the highest germination rate and dry weight of shoot and root. It also seems to concentrations above 150 mM sodium nitroprusside, toxic effects on the cells induce nitric oxide.
Iranian Journal of Seed Sciences and Research
University of Guilan
2476-3780
5
v.
3
no.
2018
77
88
https://jms.guilan.ac.ir/article_2936_f81ea571293ebfa8fbb547f2b9ec1b76.pdf
dx.doi.org/10.22124/jms.2018.2936
Effect of seed priming on germination parameters, electrical conductivity, potassium leakage, and some seedling characteristics of pinto bean (Phaseolus vulgaris Var. Khomeyn) aged seed
Mojgan
Amiriani
Dept. of Agronomy, MSc. Student in Agronomy, Faculty of Agriculture, Lorestan University
author
Hamid Reza
Eisvand
Agrotechnology Department, Lorestan University
author
Mohammad
Feizian
Dept. of Soil Science, Faculty of Agriculture, Lorestan University
author
Dariush
Goodarzi
Dept. of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University
author
text
article
2018
per
Seed aging reduces seed quality, germination, and resulted seedling. This experiment was conducted with the aim of investigating possibility of mitigating the harmful effects of seed aging in pinto bean as a factorial experiment based on a completely randomized design with three replications in 2016 in faculty of agriculture, Lorestan University. Factors were included seed aging in two levels (aged and non-aged), and priming in five levels (control, hydropriming, gibberellin 100 ppm, salicylic acid 14 ppm, gibberellin 100 and salicylic acid 14 ppm). The results showed that germination percentage and rate, seed vigor index and germination uniformity decreased by seed aging, but the electrical conductivity, potassium leakage, calcium leakage and time to 50% of germination increased. Seed priming, especially hormonal priming improved seed quality. Gibberellin 100 ppm was the most effective treatment in reducing potassium leakage and increasing germination speed.
Iranian Journal of Seed Sciences and Research
University of Guilan
2476-3780
5
v.
3
no.
2018
89
101
https://jms.guilan.ac.ir/article_2937_3736cbc6b387bfb057f1f2bdacf43fed.pdf
dx.doi.org/10.22124/jms.2018.2937
The effects of seed coating treatments on seedling emergence and growth of rapeseed and the growth of pathogenic fungi
Shirin
Taghi zoughi
دانشجوی کارشناسی ارشد
author
Elias
Soltani
عضو هیات علمی دانشگاه تهران
author
Iraj
Allah dadi
عضو هیات علمی دانشگاه تهران
author
Reza
Sadeghi
عضو هیات علمی دانشگاه تهران
author
text
article
2018
per
In order to investigate the effect of accelerated ageing and priming on biochemical and germination indices of cumin, a two factors experiment was conducted based on completely random design with four replications. Experimental factors were included seed deterioration in four levels (control, 12, 24, 36 hours) and priming in five levels (urea and KH2PO4 in two Osmotic potentials of -1 and -1.5 Mpa, and hydro-priming with distilled water). The highest catalase and ascorbate peroxidase activities as well as soluble protein content were observed in the non-deteriorated seeds primed with KH2PO4 -1.5 Mpa. Also that the highest germination percentage (86%) and germination rate (2.34 seed day-1), seedling length vigor index (4.90) and the lowest mean germination time (8.4 day) related to the non-deteriorated seeds primed with KH2PO4 -1.5 Mpa. The biggest shoot (3.52cm) and root (2.32cm) length attained from not deteriorate seeds that primed with urea -1.5 Mpa. Generally, the results showed that priming by KH2PO4 with osmotic potential of -1.5 Mpa could improve vigor of deteriorated seed of cumin.
Iranian Journal of Seed Sciences and Research
University of Guilan
2476-3780
5
v.
3
no.
2018
103
115
https://jms.guilan.ac.ir/article_2938_6b203e0acbc1b70b01571cc11f03a29f.pdf
dx.doi.org/10.22124/jms.2018.2938
The influence of seed pre-treatment with Rhizobium and plant growth promoting rhizobacteria on nodulation and nitrogen fertilizer optimization in soybean (Glycine max L.)
Reza
Taghizadeh
Department of Agriculture and plant breeding, Astara branch, Islamic Azad University, Astara, Iran.
author
text
article
2018
per
In order to study the influence of soybean (Glycine max L.) seed pre-treatment with Rhizobium japanicum and plant growth promoting rhizobacteria (PGPR) on nodulation and nitrogen fertilizer optimization, a spilt plot experiment based on randomized complete block design with three replications was conducted in 2011 at the research farm of the Islamic Azad University, Ardabil Branch. Factors were: nitrogen fertilizer rates at four levels (0, 25, 50 and 75 kg urea/ha) in the main plot and soybean seed pre-treatment with Rhizobium and plant growth promoting rhizobacteria (PGPR) in four levels containing of without seed inoculation as control, inoculation seed with Rhizobium japanicum, Azotobacter chroococcum strain5, Azosprilium lipoferum strain OF. The results showed that with increasing the amount of fertilizer, fertilizer use efficiency was reduced. Maximum number (16) and dry weight (16 mg) of nodules was obtained in seed inoculation with Rhizobium japanicum along with application of 50 kg urea/ha and minimum of these traits were observed in without of urea fertilizer application and seed inoculation. So, the highest plant height (117.22 cm), number of pod per plant (54.72), grain hundred weight (28.26 g) and grain yield per area (267 g/m2) was obtained in Rhizobium japanicum whit application of 75 kg urea/ha. At the level of 75 kg urea/ha fertilizer, inoculation with Azosprilium, Azotobacter and Rhizobium bacteria increased seed yield (14.4, 21.4 and 40.6 percent, respectively) compared to non-inoculation. Based on the results, it was concluded that application of 75 kg urea/ha in seed inoculation with Rhizobium can be recommended for profitable soybean production in the study area.
Iranian Journal of Seed Sciences and Research
University of Guilan
2476-3780
5
v.
3
no.
2018
117
129
https://jms.guilan.ac.ir/article_2939_a5b83ed53aea59444091e1ff56848ec3.pdf
dx.doi.org/10.22124/jms.2018.2939
Does seed quality affect the thermal thresholds of seed germination? Case Study: Farm saved seeds of wheat (Triticum aestivum L.) Cultivar Chamran
Abolfazl
Derakhshan
دانشجو دکتری زراعت دانشگاه کشاورزی و منابع طبیعی رامین خوزستان
author
SeyedAmir
Moosavi
Department of plan production and Genetics, faculty of Agriculture, Ramin University of Agriculture and Natural Resources, Mollasani, Khuzestan, Iran
author
text
article
2018
per
The models based on thermal time concept are useful tools for describing germination in relation to the time and temperature. The aim of this study was to determine the effect of seed quality on the thermal germination thresholds of wheat seed lots. For this purpose, germination of 16 seed lots was evaluated using the thermal time models based on Normal and Log-Normal distributions according to two different approaches. In the first approach, the base temperature (Tb) was assumed constant for all seeds in the population and the variation of germination timing among seeds was attributed to the variation in thermal time (θT) required for germination of each given fraction. In the second approach, the θT required for germination of the whole population was assumed to be constant and the variation of germination timing among seeds was attributed to the variation of their Tb. Goodness of fit criteria showed that the prediction accuracy of thermal time models in the first approach was more than the second approach. According to the first approach, Log-Normal distribution in comparison with normal distribution gave better and more accurate fits to the germination time courses of wheat seed lots in response to temperature. Seed quality of wheat seed lots significantly affected their maximum germination, cold tolerance threshold, rate and uniformity of germination. Based on predictions of Log-Normal thermal time model, estimates of maximum germination, Tb, thermal time requirement for start of germination and Standard deviation of θT distribution ranged from 73 to 99%, 0.26 to 3.68 C, 250.32 to 590.04 C h and 3.74 to 6.49 C h depending on the seed lot, respectively.
Iranian Journal of Seed Sciences and Research
University of Guilan
2476-3780
5
v.
3
no.
2018
131
142
https://jms.guilan.ac.ir/article_2940_ea1c77cd684a4884f1cb0aadcbed06f9.pdf
dx.doi.org/10.22124/jms.2018.2940